荧光镜的

Distribute stock around key specialized subject full automatic number of CR of smooth machine of X of appearance of ration of fluorescence of biochemical analyzer, PCR-RJ3000, C arm, Germany is become like system, Ximen child CT, color Duopule is supersonic bronchus of appearance, fiber gastroscope of fiber of lens, electron,, to can last of the hospital development laid solid foundation.

围绕重点专科配备有全自动生化分析仪、PCR-RJ3000荧光定量仪、C型臂X光机、德国CR数字成像系统、西门子CT、彩色多普勒超声仪、纤维支气管镜、电子纤维胃镜、人工肝支持系统、FS-CIZIY-A型腹水超滤治疗仪、肝病治疗仪、肾透析系统、心电工作站等专科先进设备，为医院的可持续发展奠定了坚实的基础。

This dissertation is divided into three parts. The first part is studies on calcium distribution changes during spermatogenesis, epididymal maturation, capacitation, acrosome reaction, oocyte maturation and egg fertilization using in situ precipitation by potassium antimonate and Ca〓 fluorescent probes at electron and light microscope levels. The main results are: 1 Ca〓 was probably involved in the condensing process of nucleoplasm during spermatogenesis.

本论文分为三个部分，第一部分主要是采用焦锑酸钾原位定钙法和Ca〓荧光染料在电镜和光镜水平对哺乳动物精子发生、附睾成熟、获能、顶体反应及卵母细胞成熟和卵受精过程中Ca〓的分布变化规律进行了系统的研究。

The test was divided into three groups randomly as yh-16 - experiment group, pbs- blank control group and rpmi-1640 medium - positive group.③in the following 24h、48h and 72h interventions, the morphous of ec was observed by photology inverted microscope, the cytoactive was detected by method of mtt and the cell cycle was investigated by flow cytometry.the expression of vegf was dectected by real-time fluorescent quantitate polymerase chain reaction(real-time pcr).

以有效浓度的yh-16作用于ec，在不同时相点光镜下观察ec形态，mtt检测细胞活性，流式细胞术检测细胞周期，实时荧光定量pcr检测vegf的表达量。

Results: The untreated cells were spindle-shaped adherent cells.

在镜下，观察MSCs在诱导前后的形态变化；用免疫荧光染色检测酪氨酸羟化酶的表达。

Methodsthe visual acuity, intraocular pressure and fundus were examined on 92 eyes on day 1, 3, 7, 30, 60 and 90 after intravitreous injection of ta. fundus fluorescence angiography and optical coherence tomography were checked before and on day 30 after the injection.

对玻璃体腔注射ta的92只眼分别于术后第1、3、7、30、60、90天行眼压、视力、裂隙灯及间接眼底镜检查，术前及术后30 d行眼底血管荧光造影及光学相干断层扫描。

ABSTRACT］ObjectiveTo observe the treatment and prognosis of complications caused by intravitreous injection of triamcinolone acetonide. MethodsThe visual acuity, intraocular pressure and fundus were examined on 92 eyes on day 1, 3, 7, 30, 60 and 90 after intravitreous injection of TA. Fundus fluorescence angiography and optical coherence tomography were checked before and on day 30 after the injection.

目的 观察玻璃体腔注射曲安奈德术后常见并发症的治疗及预后方法对玻璃体腔注射TA的92只眼分别于术后第1、3、7、30、60、90天行眼压、视力、裂隙灯及间接眼底镜检查，术及术后30 d行眼底血管荧光造影及光学相干断层扫描。

Results: observed typical changes of apoptosis(chromosome condensation, karyorrhexis, apoptotic body ) by TEM and Fluorescence microscopy. The number of apoptosis was increased with elevation of LPS concentration under the microscope.

结果 Hoechst荧光染色及电镜观察到脂多糖处理后滋养细胞染色体浓缩，细胞核裂解，见凋亡小体等典型凋亡表现，镜下随着脂多糖浓度的增加凋亡细胞数量显著增加。

Results Infected by this peptide, cell viability decreased 28.9%. Under light microscope, cells were shrinked and rounded, many cells were divorced from plate wall, some neuraxon shortened and broke. Apoptotic cells which nucleolus shrinked and rounded could be coloured orange by fluorescent colouration. Under electron microscope, chromatin gathered along the inside of the nuclear membrane, vacuole bodies appeared. Apoptotic peak was detected by flow cytometry and the ladder band appeared in DNA electrophoresis.

结果：细胞接触肽段后存活率下降28.9％；光镜下可见细胞贴壁不良，胞体缩小，细胞突起断裂缩短；荧光染色可见细胞突起缩短、胞核固缩、胞质染成橘红色的凋亡细胞；电镜下可见胞质中出现空泡样结构，细胞染色质浓集于核膜内侧并裂解成碎块状；流式细胞仪检测细胞出现亚二倍体峰，DNA电泳出现梯状带。

Objecties: To analyze the microscopic autofluorescence structure of normal and pathologic bronchial mucosae using FCFM during bronchoscopy.

目的：通过支气管镜检查法，应用FCFM，分析正常和病态的支气管粘膜的自发荧光微观结构

HE, Hoechest 33258 fludrescence staining and immunohistochemistry of Bcl-2 protein were used to illustrate the osteocyte apoptosis in femoral neck specimens from 10 cases of normal control and 30 cases of femoral head fractures and traumatic femoral head necroses, which were divided into early, middle and advanced stages.

方法］采用光镜观察、Hoechest33258荧光染色及Bcl-2蛋白免疫组化染色对股骨颈骨折和创伤性股骨头坏死患者共30例和10例正常对照的股骨头标本进行组织学观察、骨细胞凋亡和Bcl-2蛋白检测。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。