荧光镜的

The result of framework protein and lysosome marked with fluorescence was observed in sym-acorched laser scaning microscope: red fluorescent β-tubulin and green fluorescent eathepsin in nerve cell were seen in distribution in the same sample. The distribution of both was the same, and was located in around nucleus in the early of differentiation, and in cytoplasm and apophysis in the maturity of differentiation.

共焦激光扫描显微镜镜观察荧光双标的骨架蛋白和溶酶体的染色结果：在同一标本上，不同波长的单光激发可分别见到发红色荧光的β-tubulin和发绿色荧光的组织蛋白酶D在神经元内的存在及分布情况，二者的分布区域大致相同，神经元分化初期，较集中分布在核周附近，神经元分化成熟则分布于胞质及突起内。

Objective To investigate the clinical features, causes of blindness and diagnosis of Vogt Koyanagi Harada syndrome Methods The data of 157 patients with VKH syndrome were reviewed and analyzed Patients were carefully examined with slit lamp, ophthalmoscope, three mirror lens, fundus fluorescein angiography, indocyanine green angiography and HLA typing Results Headache was noted in 73 5% of these patients Simultaneous involvement of both eyes occurred in 80 8% of these patients Chroiditis,papilledema and edema of the retina adjacent to the optic nerve were noted in 100% of these patients in the posterior uveitis stage, whereas recurrent granulomatous anterior uveitis (98 4%),"sunset glow" fundus (95 8%) and Dalen Fuchs nodules (71 2%) were the common ocular findings in the recurrent anterior uveitis stage The common causes of blindness were papillitis, exudative retinal detachment and complicated cataract in the posterior uveitis stage, anterior uveal involvement stage and its recurrent stage Poliosis (36 3%) and alopecia (35 0%) were the most common extraocular findings Early irregular patches of fluorescence, followed by localized hyperfluorescent spots were the typical findings of FFA Dilation of choroidal vessels and leakage of ICG from the choroidal vessels were the common ICGA findings The prevalence of HLA DR4 and HLA DRw53 in patients (54 9% and 71 8% respectively) was significantly higher than that in controls (14 7% and 38 2% respectively) Conclusions VKH syndrome is characterized by chroiditis, papillitis or neuroretinitis in the posterior uveitis stage, followed by a generalized uveitis with a typical recurrent granulomatous anterior uveitis Extraocular findings and relevant examinations including FFA, ICGA and HLA typing are helpful to the diagnosis of VKH syndrome

目的探讨Vogt-Koyanagi-Harada综合征患者的临床特征、盲目原因及诊断等有关问题。方法对在1996年1月至2000年12月间就诊资料完整的157例VKH综合征患者进行回顾性分析，并对裂隙灯、眼底镜、三面镜、荧光素眼底血管造影(fundus fluorescein angiography，FFA)、吲哚青绿血管造影(indocyanine green angiography，ICGA)及人类白细胞抗原分型等检查结果进行分析。结果 VKH综合征最常见的前驱症状为头痛(102例，73.5%)，双眼同时患病118例(80.8%)；后葡萄膜炎期眼部主要表现为脉络膜炎、视乳头及附近视网膜水肿(100.0%)；前葡萄膜炎反复发作期眼部表现为复发性肉芽肿性前葡萄膜炎(128例，98.4%)、晚霞状眼底改变(95.8%)及Dalen-Fuchs结节(71.2%)；后葡萄膜炎期、前葡萄膜受累期及前葡萄膜炎反复发作期导致盲目的主要原因分别为视乳头炎、视网膜脱离及并发性白内障；毛发变白(36.3%)及脱发(35.0%)是最常见的眼外表现；炎症活动期FFA典型表现为斑驳状高荧光，ICGA发现脉络膜血管扩张、通透性增高等改变；VKH综合征患者HLA-DR4及HLA-DRw53的阳性率(54.9%及71.8%)显著高于正常对照组(14.7%及38.2%)。结论 VKH综合征患者在后葡萄膜炎期眼部典型表现为双侧脉络膜炎、视乳头炎或神经视网膜炎，随后出现以反复发作的肉芽肿性前葡萄膜炎为特征的全葡萄膜炎。眼外症状及相关的辅助检查包括FFA、ICGA 及HLA分型等有助于VKH综合征的诊断。

Were done and no positive reaction was found.5. The DNA of the young leaves of 132 Bt transgenic plants was extracted to do PCR Five positive inversion plants were obtained and the inversion rate was 3.8%.6. The DNA of fire positive inversion plants obtained in Dl generation was extracted and tested. Two stable positive inversion plants were obtained in D2 generations.7 The way of flourescent mirror flat was used to observe the pollen tube extension circumstance in the flower post after self-pollination.

检测了132株，没有发现阳性反应。5、对转入Bt基因的132株植株的幼叶提DNA，作PCR检测。D1代获得5株阳性转化植株，分别是90035的4株，85—593的1株，转化率为3.8％。6、将D1代获得5株阳性转化植株的种子在温箱中发芽，提取DNA检测，得到D2代稳定遗传的阳性转化植株2株。7、应用荧光镜制片法观察大豆自花授粉后花粉管在花柱中延伸情况。

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目前，医院拥有HA-100前列腺腔内场效消融系统、前列腺电解治疗仪、磁振聚焦治疗系统、SW性功能康复治疗仪、美国CRS电容场治疗系统、螺旋水疗治疗系统、固态源治疗系统、显微通脉技术仪、德国"狼"牌宫腔镜、腹腔镜、美国GE彩超、TCT早期宫颈癌筛选系统、美国COOK导丝、"海扶"聚焦超声系统、美国LEEP、德国生物氧治疗系统、乳腺远红外检测仪、芬兰乳腺钼靶、可视人流系统、中药全身汽疗治疗舱、日本数码阴道镜、心电监护仪、全套生化检测设备、全电脑肛肠治疗系统、美国DNR失眠监测仪、儿童注意力缺陷诊断仪、全电脑荧光微量元素分析仪、JAC-CPT 感觉综合训练仪等一大批国内外最先进的诊疗设备，以及以色列飞顿Ⅱ号美肤工作站、SHR超级冰点脱毛系统、ASTER冰电波除皱系统、ASD可视祛斑治疗仪、光子嫩肤仪、生物电美白仪、聚能震波吸脂仪、意大利微晶祛疤仪、新型半导体激光美容系统、整形美容术前设计系统等一整套韩国先进齐全的医学整形美容设备。

To investigate the fluorescence dynamic change of protoporphyrin IX in bladder carcinoma cell induced by 5-ALA and the influence of 5-ALA concentration and pH on fluorescence of PpIX. 2. To discuss the biodistribution and express of PpIX in bladder wall of different lesions by method of instilling of 5-ALA into bladder cavity. 3. To discuss the clinical value of the fluorescence diagnosis in early detection of bladder cancer and the radicality of bladder tumor resection under fluorescence cystoscopy.

本研究目的：1了解5-ALA诱导膀胱癌细胞内生原卟啉IX荧光随时间的动力学变化及5-ALA的浓度、pH值对产生PpIX荧光的影响。2通过膀胱灌注5-ALA探讨其所诱导内生PpIX荧光在膀胱上皮中分布及不同病变表达上的差异。3探讨5-ALA诱导荧光膀胱镜检对发现早期膀胱癌的临床应用价值及在荧光膀胱镜下膀胱肿瘤电切治疗的彻底性。

The onset of EGFP fluorescence and its distribution in the Wistar rat corneas were determined through fluorescence stereoscope.

建立大鼠异体角膜移植模型，用含rAAV1-EGFP转染液的培养基在37℃孵育SD大鼠角膜植片同时浸泡缝线18h，再用此缝线间断缝合，将植片移植到大鼠角膜植床，术后通过荧光体视镜观察Wistar大鼠角膜出现EGFP荧光的起始时间及分布情况。

Methods A lamellar flap was produced in the rabbit cornea with a microkeratome. The flap interface in whole corneas and corneal beds were soaked in the rAAV –EGFP solution for 10 min, BSS solution was applied in the control group. The corneas were harvested at day 1, 3, 14, and 21 after application for the structure analysis of cornea by HE staining. Expression of the transferred gene was monitored by fluorescence stereoscope and laser confocal microscope. Results After 3d of infection with rAAV-EGFP ,slight fluorescence was detected in rabbit cornea by fluorescence stereoscope .

微型角膜刀制作兔角膜基质瓣；转染组用含rAAV－EGFP转染液浸泡角膜瓣和角膜基质床10 min，对照组用等量BSS液浸泡角膜瓣和角膜基质床10 min，不同时间点(1、3、7、14、21d)通过荧光体视镜观察角膜出现EGFP荧光的起始时间及分布情况，收集角膜，其中一半用激光共聚焦显微镜观察、照相，另一半固定后行HE染色。

Methods:15 cases of conjuctivitis were detected for the trachoma chlamydia by fluoroqenic quantitative PCR and super microscope diagnosis system.

利用荧光定量聚合酶链反应和超高倍显微诊断系统镜检检测 15例结膜炎患者的沙眼衣原体。

Escherichia coli O157:H7; viable but non culturable state; acridine orange direct count; direct viable count

H7；活的非可培养状态；吖啶橙荧光显微镜直接计数法；活菌直接镜检计数法

Methods The visual acuity, intraocular pressure and fundus were examined on 92 eyes on day 1, 3, 7, 30, 60 and 90 after intravitreous injection of TA. Fundus fluorescence angiography and optical coherence tomography were checked before and on day 30 after the injection.

对玻璃体腔注射TA的92只眼分别于术后第1、3、7、30、60、90天行眼压、视力、裂隙灯及间接眼底镜检查，术前及术后30 d行眼底血管荧光造影及光学相干断层扫描。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。