荧光镜

Results: The untreated cells were spindle-shaped adherent cells.

在镜下，观察MSCs在诱导前后的形态变化；用免疫荧光染色检测酪氨酸羟化酶的表达。

Methods:15 cases of conjuctivitis were detected for the trachoma chlamydia by fluoroqenic quantitative PCR and super microscope diagnosis system.

利用荧光定量聚合酶链反应和超高倍显微诊断系统镜检检测 15例结膜炎患者的沙眼衣原体。

Escherichia coli O157:H7; viable but non culturable state; acridine orange direct count; direct viable count

H7；活的非可培养状态；吖啶橙荧光显微镜直接计数法；活菌直接镜检计数法

Rationale: Fibered confocal fluorescence microscopy is a new technique that produces microscopic imaging of a liing tissue through a 1-mm fiberoptic probe that can be introduced into the working channel of the bronchoscope.

原理：纤维共聚焦荧光显微镜这项新技术，是通过将1mm的纤维光学探头插入支气管镜的操作管道，进行活体组织的显微成像

Cases healthy subjects served as control. In addition, density of glomerular matrix membrane and quantity of cell in glomeruli were measured with CMIAS image analysis system by computer. Global sclerosis, cellular crescents, fiber or fibrocellular crescents were measured with Memphis scope. Tubulointerstitial lesions were examined with semi-quantitative grades (including O-III grades).Results Compared to normal control, there was a significant increase of urinary TGF-betaK IL-6 and Col-lV levels in patients with IgAN (p. 01). There were also significantly positive correlation between the levels of urinary TGF-betaK Col-IV and density of glomerular matrix membrane ., interstitial fibrosisp.

采用酶联免疫吸附法测定尿TGF-β1(Transforming growth factor-β1)、尿IL-6(interleukin-6)和尿Col-Ⅳ(type Ⅳ Collagen)；采用免疫组织化学法检测肾组织TGF-β1、Col-Ⅳ表达；应用CMIAS多功能真彩色病理图像分析系统对肾小球基质基底膜面密度和细胞个数进行半定量测定；对球性硬化、节段硬化、细胞新月体及纤维或纤维细胞新月体所占肾小球百分数和肾小球IgA沉积免疫荧光强弱的判断采用Memphis法进行评分；肾小管间质病变程度采用光镜下半定量分级法(包括0-Ⅲ级)。

Electron donating substituent CH〓, OCH〓 and 4, 5-benzo make λ〓 red shift, electron with -drawing substituent F, Cl and CF〓 make λ〓 hypsochromic shift; b The W〓 is bigger than relative symmetrical carbocyanines, and the ratio of 0-1＇ and 0-0＇ transition intensity increase with the increase of σ〓; c The absorption and the fluorescence spectra are mirror image, and the λ〓 have a good linear relationship; d The Stokes shift △λ〓 and the Hammett σ〓 constant have a good linear relationship.

研究了染料的光物理性质，①苯环上取代基影响染料的λ〓和摩尔消光系数，相对于H，给电子取代基CH〓、OCH〓、4，5-benzo使λ〓红移，摩尔消光系数减小，吸电子取代基F、Cl、CF〓使λ〓蓝移，摩尔消光系数减小；②化合物吸收峰的半峰宽较相应的对称染料的大，且振动肩部与最大吸收峰的强度比随σ〓增大而增大；③吸收光谱与荧光光谱呈镜像关系，且λ〓与λ〓呈良好的线性关系；④化合物的Stokes位移△λ〓与取代基的Hammett σ〓常数呈线性关系。

Methods The visual acuity, intraocular pressure and fundus were examined on 92 eyes on day 1, 3, 7, 30, 60 and 90 after intravitreous injection of TA. Fundus fluorescence angiography and optical coherence tomography were checked before and on day 30 after the injection.

对玻璃体腔注射TA的92只眼分别于术后第1、3、7、30、60、90天行眼压、视力、裂隙灯及间接眼底镜检查，术前及术后30 d行眼底血管荧光造影及光学相干断层扫描。

Methodsthe visual acuity, intraocular pressure and fundus were examined on 92 eyes on day 1, 3, 7, 30, 60 and 90 after intravitreous injection of ta. fundus fluorescence angiography and optical coherence tomography were checked before and on day 30 after the injection.

对玻璃体腔注射ta的92只眼分别于术后第1、3、7、30、60、90天行眼压、视力、裂隙灯及间接眼底镜检查，术前及术后30 d行眼底血管荧光造影及光学相干断层扫描。

ABSTRACT］ObjectiveTo observe the treatment and prognosis of complications caused by intravitreous injection of triamcinolone acetonide. MethodsThe visual acuity, intraocular pressure and fundus were examined on 92 eyes on day 1, 3, 7, 30, 60 and 90 after intravitreous injection of TA. Fundus fluorescence angiography and optical coherence tomography were checked before and on day 30 after the injection.

目的 观察玻璃体腔注射曲安奈德术后常见并发症的治疗及预后方法对玻璃体腔注射TA的92只眼分别于术后第1、3、7、30、60、90天行眼压、视力、裂隙灯及间接眼底镜检查，术及术后30 d行眼底血管荧光造影及光学相干断层扫描。

Methods SECs were isolated by collagenase perfusion combined with isopycnic centrifugation in a two-step percoll gradient and selective adherence, and identified by morphologic observation under light microscope, by immunocytochemistry for CD14 and Ⅷfactor related antigen, by immunofluorescence staining for RECA-1 and fenestrations under SEM.

方法用胶原酶灌注结合Percoll密度梯度离心加选择性贴壁的方法分离SEC；用光镜形态学、Ⅷ因子和CDl4免疫细胞化学染色及RECA.1单抗间接免疫荧光法及SEM鉴定SEC；采用TEM及AnnexinV联合PI双染的方法检测SEC的凋亡。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。