荧光镜

METHODS: HUVECs were identified by microscopic observation of morphology and immunoflourescence staining with a monoclonal mouse antihuman vWF antibody.

采用胰蛋白酶消化法体外分离健康人脐静脉内皮细胞，通过光镜下形态观察和抗vWF免疫荧光染色对培养细胞进行鉴定。

The result of framework protein and lysosome marked with fluorescence was observed in sym-acorched laser scaning microscope: red fluorescent β-tubulin and green fluorescent eathepsin in nerve cell were seen in distribution in the same sample. The distribution of both was the same, and was located in around nucleus in the early of differentiation, and in cytoplasm and apophysis in the maturity of differentiation.

共焦激光扫描显微镜镜观察荧光双标的骨架蛋白和溶酶体的染色结果：在同一标本上，不同波长的单光激发可分别见到发红色荧光的β-tubulin和发绿色荧光的组织蛋白酶D在神经元内的存在及分布情况，二者的分布区域大致相同，神经元分化初期，较集中分布在核周附近，神经元分化成熟则分布于胞质及突起内。

Crowds of authigenic kaolinite and automorphic pyrite as well as blue fluorescence of organic phase under microscope.

如光学镜下的自生高岭石晶簇和自形黄铁矿的出现以及有机相的蓝色荧光色调等。

We have many series of advanced medical instruments, such as HA-100 prostata cavity infield effect melts systematically, prostata electrolysis cures instrument, Magnetism shakes the system focusing treating, SW nature dysfunction recovery instrument, USA CRS capacitance cures system, the helix hydrotherapeutics melts systematically, third generation visible involvement leads melts the system, solid state source system, micro pulse technology instrument, Germany "wolf" peritoneoscope, USA GE colour B-ultrasonography, TCT, USA COOK godet, CFZ, LEEP, Living oxygen cures system, infrared mammary glands diagnosis, CR, etc.And we also have many series of advanced medical equipment of plastic surgery, such as Israel to fly Dayton Ⅱ skin beauty workstations, SHR freezing point unhairing system, ASTER lifting ice radio system, ASD visual beauty cream treatment device, Photorejuvenation instrument, bio-power whitening kit, the liposuction wave shaped device, Eliminate scar device, a new type of semiconductor laser system, etc.

目前，医院拥有HA-100前列腺腔内场效消融系统、前列腺电解治疗仪、磁振聚焦治疗系统、SW性功能康复治疗仪、美国CRS电容场治疗系统、螺旋水疗治疗系统、固态源治疗系统、显微通脉技术仪、德国"狼"牌宫腔镜、腹腔镜、美国GE彩超、TCT早期宫颈癌筛选系统、美国COOK导丝、"海扶"聚焦超声系统、美国LEEP、德国生物氧治疗系统、乳腺远红外检测仪、芬兰乳腺钼靶、可视人流系统、中药全身汽疗治疗舱、日本数码阴道镜、心电监护仪、全套生化检测设备、全电脑肛肠治疗系统、美国DNR失眠监测仪、儿童注意力缺陷诊断仪、全电脑荧光微量元素分析仪、JAC-CPT 感觉综合训练仪等一大批国内外最先进的诊疗设备，以及以色列飞顿Ⅱ号美肤工作站、SHR超级冰点脱毛系统、ASTER冰电波除皱系统、ASD可视祛斑治疗仪、光子嫩肤仪、生物电美白仪、聚能震波吸脂仪、意大利微晶祛疤仪、新型半导体激光美容系统、整形美容术前设计系统等一整套韩国先进齐全的医学整形美容设备。

The onset of EGFP fluorescence and its distribution in the Wistar rat corneas were determined through fluorescence stereoscope.

建立大鼠异体角膜移植模型，用含rAAV1-EGFP转染液的培养基在37℃孵育SD大鼠角膜植片同时浸泡缝线18h，再用此缝线间断缝合，将植片移植到大鼠角膜植床，术后通过荧光体视镜观察Wistar大鼠角膜出现EGFP荧光的起始时间及分布情况。

Methods A lamellar flap was produced in the rabbit cornea with a microkeratome. The flap interface in whole corneas and corneal beds were soaked in the rAAV –EGFP solution for 10 min, BSS solution was applied in the control group. The corneas were harvested at day 1, 3, 14, and 21 after application for the structure analysis of cornea by HE staining. Expression of the transferred gene was monitored by fluorescence stereoscope and laser confocal microscope. Results After 3d of infection with rAAV-EGFP ,slight fluorescence was detected in rabbit cornea by fluorescence stereoscope .

微型角膜刀制作兔角膜基质瓣；转染组用含rAAV－EGFP转染液浸泡角膜瓣和角膜基质床10 min，对照组用等量BSS液浸泡角膜瓣和角膜基质床10 min，不同时间点(1、3、7、14、21d)通过荧光体视镜观察角膜出现EGFP荧光的起始时间及分布情况，收集角膜，其中一半用激光共聚焦显微镜观察、照相，另一半固定后行HE染色。

Through this issue, we found that with 2% NaOH-NALC digested within 10 minutes in the rapid decomposition of the viscous sputum, in ensuring the effectiveness of digestion at the same time not too much because of the role of NaOH to kill bacteria (time to 20 minutes), and would not affect the role of magnetic particles; quantitative simulation of self-made through the application of clinical samples, we compared the acid-fast stain and auramine O staining of the detection limit, auramine O fluorescence staining against the backdrop of a clear dark green light acid-fast stain of Mycobacterium tuberculosis than the white background of the red eye-catching TB is more capacity, auramine O staining in the detection limit of 0.05 to 0.001 between Maxwell, higher than the acid-fast stain of Michael's 0.05 units, The former is more sensitive; In addition, the accumulation of magnetic nanoparticles of the samples after the smear, in the microscope show the number of TB was significantly higher than that of the specimens has not been enriched.

通过本次课题，我们发现用2%NaOH-NALC消化液可在10分钟内迅速裂解痰液的粘性介质，在保证消化效果的同时不会由于NaOH过强的作用杀死细菌（时间控制在20分钟内），亦不会影响磁性颗粒的作用；通过应用自制定量模拟临床标本，我们比较了抗酸染色和金胺O荧光染色的检出限，金胺O荧光染色黑暗背景下发出明显绿色亮光的结核杆菌较抗酸染色白色背景中的红色结核杆菌更容醒目，金胺O荧光染色的检出限在0.05~0.001个麦氏单位之间，高于抗酸染色的0.05个麦氏单位，前者敏感度较高；另外，经过磁性纳米颗粒富集作用后的标本涂片，在镜下显示其结核杆菌数量明显高于未经过富集的标本。

3PH was done in group M and T. Then the rats in B group and M group were injected normal saline via portal vein, and those in T group were injected with SHCs suspension. Four week later, the level of serous alanine aminotransferaseand glutamic oxalacetic transaminase were tested, and liver pathological changes observed. Frozen sections of T group were observed under fluorescent microscope.

将实验用同一近交系SD大鼠30只随机平均分成空白对照组、损伤造模组和SHCs移植组。B组腹腔注射生理盐水，M组、T组腹腔注射CCl4.24 h后戊巴比妥腹腔麻醉，消毒开腹，M、T组行2/3肝切，B、M组门静脉注入生理盐水，T组门静脉注入经膜荧光标记SHCs悬液。4周后，采血测血清谷丙转氨酶、谷草转氨酶，并取病理标本做冰冻切片荧光显微镜下观察及HE染色普通光镜下观察。

The invention provides an ultra-short length erbium-ytterbium co-doped high gain glass fiber superfluorescence light source which includes: a semiconductor laser 1, a coupling lens system 2 (including a collimating lens 3 and a focusing lens 4), a signal light total reflective mirror 5, a high gain fiber 6 a pumped light input terminal is a vertical cutting terminal 8 in figure (a structure and is a bevel terminal 7 in figure structure, a superfluorescence output terminal is the bevel terminal 7 in figure and.

一种超短长度铒镱共掺高增益玻璃光纤超荧光光源，包括：半导体激光器1，耦合透镜系统2(包括准直透镜3和聚焦透镜4)，信号光全反镜5，高增益光纤6泵浦光输入端在图(a结构中为垂直切割端8，在图结构中为斜角端7，超荧光输出端在图、中均为斜角端7四部分。

Through this topic, we discover with the 2%NaOH-NALC digesting fluid may in 10 minute in rapid decomposition sputamentum resisting medium, during guarantee digestion effect not because the NaOH strong function will have killed the bacterium (timing control in 20 minutes), also will not affect the magnetic pellet the function; Through the application self-restraint quota simulation clinical specimen, we have compared the acid-fast stain and the pyoktanin yellow O fluorescence dye pick out limit, the pyoktanin yellow O fluorescence dyes the dark background to after-crop the obvious green bright the bacillus tuberculosis to compare in the acid-fast stain white background the red bacillus tuberculosis to accommodate strikingly, the pyoktanin yellow O fluorescence dyes picks out limits between 0.05~0.001 Mai unit, is higher than the acid-fast stain 0.05 Mai unit, the former sensitivity is high; Moreover, concentrates the function after magnetic nano particles the specimen smear, under the mirror demonstrated that its bacillus tuberculosis quantity is higher than the specimen which obviously has not passed through concentrates.

通过本次课题，我们发现用2%NaOH-NALC消化液可在10分钟内迅速裂解痰液的粘性介质，在保证消化效果的同时不会由于NaOH过强的作用杀死细菌（时间控制在20分钟内），亦不会影响磁性颗粒的作用；通过应用自制定量模拟临床标本，我们比较了抗酸染色和金胺O荧光染色的检出限，金胺O荧光染色黑暗背景下发出明显绿色亮光的结核杆菌较抗酸染色白色背景中的红色结核杆菌更容醒目，金胺O荧光染色的检出限在0.05~0.001个麦氏单位之间，高于抗酸染色的0.05个麦氏单位，前者敏感度较高；另外，经过磁性纳米颗粒富集作用后的标本涂片，在镜下显示其结核杆菌数量明显高于未经过富集的标本。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。