荧光计

In this paper,the principle and makeup of optically stimulated luminescence dosimeter is described,and a measurement for radiation is carried,some actual problem is discussed.

介绍了光致荧光剂量计的原理和组成，并进行了辐射剂量测量，探讨了应用中存在的一些实际问题。

A software and a relevant method are designed and set up for the calculation of the relative content of the inactive PS Ⅱ center, by using which this study can be accomplished A new non-modulation fluorometer with its data acquisition and date analysis software is also designed and assembled

此外，也参加了新的非调制式动力学荧光计的研制及其软件的编写。

A new type of the non-modulation fluorometer has been built which has higher signal/noise ratio. responsibility and accuracy, much lower power consumption, and faster data acquisition rate, etc. This new software witten in C language can run in the IBM PC compatible computer.

新研制的荧光计的软件用C语言编写，可在IBM PC兼容机上运行，采样速度最快可达25μs/点，对Fo和Fi等参数的测定更加可靠和精确。

Besides of retaining most functions of the old software, the new one has higher data acquistion rate up to the maximum of 25μs/point for determining Fo and Fi etc. more accurately. This fluorometer has reached the advanced level of the same kind in the world.

新荧光计从软、硬件两方面进行了彻底地更新，具有高信噪比、高响应、高精度、低功耗等优点，其性能已达到国际同类产品的先进水平。

A data acquisition and data analysis software for the fast fluorscence kinetics fluorometer of our laboratory is redesigned and recompiled using assembly and BASIC language. The data acquisition rate in the fast rising part of the fluorescence kinetics curves is one order faster (100μs/point) than the old one. It can measure fluorescence parameters, such as Fo and Fi more accurately for calculation of the relative content of inactive PSⅡ, etc.

用N80-BASIC语言结合汇编语言重新编写了本室快速叶绿素动力学荧光计的测控程序，使快速荧光上升曲线的采样速度提高了一个数量级（达到100μs／点），可对Fo、Fi等关键荧光参数进行精确测定，为无活性PS Ⅱ中心相对含量的准确测定奠定了基础。

The company's main products: color detection instruments: Glossiness Miriam, Miriam color spectrophotometry, standard light source for color light box, transmissioní°Miriam, Miriam orange peel, fresh young tester; surface coating physical properties testing instruments: Coating thickness, wet film thickness, temperature tracker, surface humidity instrument, dewpoint meter, roughness instrument, electric spark pinhole leak detectors; Detect mechanical properties of surface coating equipment: zoned grid, and pencil hardness tester , impact tester, hardness testing sticks, Latvia Law / hydraulic adhesion tester, abrasion tester, cylinder / cone bending tester, cups sudden device, surface tension instruments, scratch pen, indentation hardness tester, etc.; paint coating equipment dedicated category: fineness board, drying time recorder, wet adhesion tester, paint ohmmeter, PH, the coating device, mixer, bead mill, mixer, spray gun, putty detector Miriam, white tester, iron cobalt colorimeter, moisture titrator; viscosity-type equipment: Viscosity Cup, density cup, bubble viscosity tube viscometer, infiltration cup rheometer, viscosity units conversion meter; laboratory standard consumables: Mitsubishi pencil tests, 3M adhesive tape test, RCA tape, rubber, the hiding power test paper for color tubes, microscopes dedicated LED ring light source, transfer ink knife and ink scraper, brush, Color Card, gray cards, zirconia beads, glass beads, pieces of cement mortar, the standard of fly ash, the standard viscosity fluid, stopwatch, chemical industry-standard compilation of manuals, paint paint testing manual; test standard Material: tin plate, steel plate, aluminum sheet, asbestos-cement pressure plate, Eterpan, glass, maple panels, MDF, copper film, polyester film, copper; measuring meter and optical instruments: digital calipers, micrometer, dial gauge a high scale, depth gauge, thickness gauge, diameter gauge, gauge block, magnifiers, microscopes, projectors, video measuring instrument; environmental test equipment: oven, constant temperature and humidity box, hot and cold circulating water bath, salt spray test chamber, high temperature resistance furnace, fluorescent UV aging chamber, xenon lamp aging chamber; electronics equipment: infrared thermometer, multimeter, clamp sheet, thermal imager, temperature and humidity meter; anti-static instruments: static tester, surface resistivity device, flat-panel monitor, anti-static footwear tester, wrist strap tester, body Tester; other types of instruments and equipment: torque tester, temperature and humidity recorder, push pull, the vibrometer, speed instrument, analytical balance, industrial Scale Units, dust particle counter, X-ray fluorescence coating thickness measurement and elemental composition analyzer, and so on.

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Firstly, the pGL3-Control vector was reconstructed , the pGL3-Enhancer vector deletious of SV40 promoter gene was obtained . Then the IFN-β promoter gene was cloned into the pGL3-Enhancer vector and pGL-IP21, the Luciferase reporter plasmid with IFN-β promoter was OK. The availability of pGL-IP21 was verified by NDV ,the inductor of IFN-β, the Luciferase activity was assayed in cells transfected with pGL-IP21 by Luminometer.

首先将pGL3-Control载体进行了改构，除去了SV40启动子基因，获得了pGL3-Enhancer载体，将获得的IFN-β启动子基因连入载体中，构建了带有人IFN-β启动子基因的荧光素酶报告质粒IP-21，并且通过实验证明所构建的质粒在干扰素的诱导剂NDV的作用下能够表达荧光素酶活性，照度计检测荧光素酶活性增强，从而验证了所构建的重组质粒的有效性。

Abstracts The effects of fixation with paraformaldehyde or glutaraldehyde and preservation with liquid nitrogen on cell size, density, phycoerythrin fluorescence value (FL2) and chlorophyll fluorescence (FL3) of picoplanton cells were studied by using flow cytometry.

摘要以流式细胞计为海洋微微型浮游生物的分析工具，研究了醛类固定剂的固定、液氮贮存对于微微型浮游生物的细胞大小、密度、藻红素荧光值(FL2)和叶绿素荧光值(FL3)的影响。

①Rat MSC and VSMC were cultured and identified, respectively. MSC were labeled with DAPI firstly, and then co-cultivated with VSMC. The changes of morphology and ultrastructure of co-cultured cells were observed. Immunfluorescence analysis was performed by using monoclonal antibodies against specific antigen.②We established the regulatable system in two steps: a stable MSC line expressing rtTA has been constructed and characterized firstly by transfected with pUHD 17-1hyg and then selected by hygromycin B; in a second step, this line was used for trandfer the AT2R gene to MSC to get the well establishing double stable MSC lines;③The expression of AT2R regulated by doxycycline was evaluated by western blot;④The MSCs were transduced into rat carotid arteries with regulatable AT2R gene after the establishment of rat carotid balloon injury restenosis model. The intimal/medial area ratio were measured by digital analysis system.

研究方法：(1)密度梯度离心法及胶原酶消化法分别培养原代大鼠MSC及VSMC，细胞共培养并行免疫荧光化学染色和透射电镜观察超微结构；(2)组成受Dox调控的哺乳动物表达系统的四种成分的转化、扩增及提纯并酶切鉴定；(3)采用常规分子生物学方法连续两个回合转染体外培养的MSC，并分别采用发光计检测不同细胞克隆萤光素酶活性改变以及RT-PCR方法检测AT2R目的基因mRNA表达情况，根据各个细胞克隆受Dox调控表达的程度，选择低背景、高诱导表达AT2R的细胞系，作为双重稳定MSC细胞系；蛋白免疫印迹法观察该细胞系在Dox调控下AT2R表达的时相性、持续性及在不同浓度Dox调控下的表达情况；(4)建立大鼠颈动脉球囊损伤动物模型，将双重稳定MSC在术中导入血管，分别于14 d、28 d进行病理切片，检测可调控AT2R对新生内膜增生的影响；采用RT-PCR免疫组织化学免疫荧光等技术观察AT2R基因在新生内膜中的表达以及细胞外基质成分表达的改变，TUNEL法检测血管组织中细胞凋亡的变化情况。

This article summarises the chemical reactions in milk during heat treatment, analyzes the properties of several indicators for heat damage , such as heat-sensitive enzymes, denatured proteins, the products of Mailliard Reaction and isomerised lactose.

本文分析了牛乳在热加工过程中的化学变化，介绍了几种牛乳热损伤监控标识物(如热敏性酶类，蛋白质变性产物，美拉德反应产物和乳糖异构化产物)的特点，并详细论述了已有的牛乳热损伤监控技术：对变性β-乳球蛋白含量的监控，对糠氨酸和乳果糖含量综合水平的监控，对羟甲基糠醛含量的监控，以及以变性蛋白质(以色氨酸的荧光强度计，FTry)和美拉德反应荧光产物的聚集量为基础，评估牛乳热加工损伤程度的FAST方法。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。