荧光素

Then PCR products were hybridized with 5 pairs of probes that immobilised on Biodyne C membrane.

通过标记在PCR上游引物5'端的荧光素显色得到结果，并将其与测序结果进行对比。

Methods Compared and contrasted the SIt (Schirmer Ⅰ test) value between open-eye group and close-eye group in 125 patients. Compared and contrasted the BUT (tear break-up time )value of 80 patients who accepted the examination with 0.125% fluorescent stain and 0.25% fluorescent stain orderly.

对125例疑诊干眼患者在睑裂开放状态下和睑裂闭合状态下行基础泪液分泌试验Ⅰ(Schirmer Ⅰest， SIt)；对80例疑诊干眼患者分别进行0.125%和0.25%荧光素钠(fluorescent， fl)测试泪膜破裂时间(Break-up Time， BUT)，对上述结果进行对照分析。

Postoperatively, B group (36 eyes) were dropped with TobraDex and artificial tears(Dextran 70 Eye Drops), and were given mydriasis and antiglaucoma eyedrops when necessary. Uncomfortable symptoms ( dry sensation, foreign body sensation,Burning sensation) were inquired, as well as tear break-up time, Schirmer test value and corneal fluorescein staining were measured 1 day preoperatively plus 3, 7, 14, 30 days postoperatively.

继续收集36例(36眼)患者全部纳入B组，手术后应用妥布霉素地塞米松眼液、眼膏和人工泪液Dextran 70 Eye Drops，必要时联合应用散瞳眼液和降眼压眼液。B组病例分别于术前1d，术后3d、7d、14d及30d详细询问患者是否存在不适症状(干涩感、异物感、烧灼感)并评分，并行泪膜破裂时间(Break-Up Time，BUT)、泪液分泌试验(Schirmer test，ST)、角膜荧光素染色(Cornea Fluorescein Staining，CFS)检查及评分。

Uncomfortable symptoms(dry sensation, foreign body sensation,Burning sensation) were inquired, as well as tear break-up time, Schirmer test value and corneal fluorescein staining were measured 1 day preoperatively plus 1, 3, 7, 14, 30 days postoperatively.

分别于术前1d，术后1d、3d、7d、14d及30d询问患者是否存在不适症状(干涩感、异物感、烧灼感)并评分，并行泪膜破裂时间(Break-Up Time，BUT)、泪液分泌试验(Schirmer test，ST)、角膜荧光素染色(Cornea Fluorescein Staining，CFS)检查及评分。

In the mice model with oxygen induced retinal neovascularzition, FFA showed permeable on retinal vessels, circuity of retinal vessels and nonperfusion areas around the optic disc.

在氧致视网膜新生血管小鼠模型，荧光素血管造影显示视网膜血管迂曲增粗，造影剂渗漏，有无灌注区形成。

The details of cloning, expression and function of biotinylated luciferase will be discussed in this paper.

本文将就生物素化荧光素酶的克隆、表达以及功能检测进行具体讨论。

AIM: To investigate the effect of PD98059, a specific repressor of extracellular signal regulated kinase1/2 (ERK1/2), on the proliferation of the murine lymphocytes. METHODS: Lymphocytes were isolated from lymphoid nodes of BALB/c mouse. After stained with carboxy fluorescein diacetate, succinimidyl ester, the cells were stimulated with polyclonal activators, concanavalin and phorbol 12,13 dibutyrate plus ionomycin.

分离小鼠淋巴结细胞，藉羧基荧光素乙酰乙酸染色，用多克隆刺激剂刀豆A蛋白或者佛波醇酯加离子霉素刺激，以流式细胞术分析PD98059对淋巴细胞增殖的影响；利用碘化丙锭染色分析PD98059对细胞周期的影响。

METHODS: After stimulated with Con A, T cells were treated with different concentrations of TMB-8 alone and its combination with cyclosporine A. The expression of CD69, the early marker of CD3(superscript +) T cell activation, was measured by FACS. The proliferation-related index was determined by carboxyl fluorescin diacetate succinmidyl ester flow cytometry. The cell-cycle distribution was analyzed by propidium iodide staining.

以Con A作为T细胞活化、增殖的刺激剂，以不同浓度的TMB-8及与环孢菌素A联合作用于该系统，用流式细胞术检测T细胞早期活化标志CD69分子的表达；以活体染料羧基荧光素乙酰乙酸染色流式细胞术，分析TMB-8在Con A刺激下小鼠淋巴细胞的增殖相关指数；以碘化丙啶染色分析细胞周期的分布情况。

A red fluorescent dye resulting from the action of bromine on fluorescein; used in cosmetics and as a biological stain for studing cell structures.

红色的荧光染料，由溴作用于荧光素而产生，用于化妆品制造并用作研究细胞结构的生物着色剂。

It was shown that the fluorescence spectra of fluorescein in the gels had a red shift in contrast to those in the solution of diphenyl ether and that the gelation thime decreased with increasing the concentration of BMDM.

研究结果发现，与荧光素在二苯醚溶液中的荧光光谱相比，在凝胶中出现了 10～25nm的红移；而且发现分子凝胶的凝胶化时间随着BMDM浓度的增加而减小。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。