荧光素

RLuciferase/Luciferin system, a new method to detect the MAPK activity in fungus, was founded and proved to be effective and perspective in the study of MAPK pathway in microorganism.

建立了一套切实可行的检测MAPK活性的荧光素酶/荧光素技术体系，并发现MAPK途径抑制剂U0126和CaM抑制剂TFP对MAPK活性都有显著的抑制作用。

Luciferin,as the light-carrier substance or the energy-transfer substance,plays an indispensability role on the luminescent system of luciferase in the biology illuminant.

在生物发光体内，荧光素作为直接释放光子的物质和能量的传递物质在荧光素酶发光体系中发挥着不可或缺的作用。

Effects of four insecticides on intestinal mucosa permeability of the wolf spider were studied by exposing spiders to insecticides and then measuring the concentration of fluorescein isothiocyanate-dextran in the spiders'blood. The results showed that FITC-D concentrations of the test groups were much more than that of the control group.

采用异硫氰酸荧光素-葡聚糖作为示踪物质，通过检测拟环纹豹蛛血液内的荧光素浓度，研究了两种生物源杀虫剂和两种化学源杀虫剂对拟环纹豹蛛肠黏膜通透性的影响。

An enzyme present in the cells of bioluminescent organisms that catalyzes the oxidation of luciferin.

荧光素□存在于生物性发光组织的细胞中，催化荧光素氧化的

Results The in vitro coupled bacterial luciferase:FMN-NADH oxidoreductase bioluminescent system was: 1mL crude extract, 27 mmol/L Dodecane 100 μL, 10 mmol/L FMN-Na 0.5 μL and 0.14 mmol/L NADH 300 μL.

利用从鳆发光杆菌提取并经部分纯化的荧光素酶和FMN-NADH氧化还原酶，通过优化体系中各底物的添加量，实现荧光素酶的体外发光。

With fluorescin as reporter molecules, the distribution and elimination of the liposome were tested in vivo in mice.

导向脂质体可以明显减慢荧光素钠在小鼠体内的吸收与消除的过程，并明显提高荧光素钠在肝组织中的分布含量。

A chemical substance present in the cells of bioluminescent organisms, such as fireflies, that produces an almost heatless, bluish-green light when oxidized under the catalytic effects of luciferase.

荧光素存在于生物性发光有机体中的一种化学物质，它在荧光素酶催化作用下氧化时发生几乎没有热量的蓝绿光

A chemical substance present in the cells of bioluminescent organisms,such as fireflies ,that produces an almost heatless,bluish - green light when oxidized under the catalytic effects of luciferase.

荧光素存在于生物性发光有机体中的一种化学物质，它在荧光素□催化作用下氧化时发生几乎没有热量的蓝绿光

MethodsThe D. bacteria - E. coli shuttle expression vector pZT17 was constructed based on plasmids of pUE30, pGBM5 and pKatCAT. Then pZT17 with lux+ from Photinus pyralis was used to transform into D. grandis and E.coli. The recombinant strains were induced separately.

以质粒pUE30、pGBM5及pKatCAT为基础，构建抗辐射菌属一大肠杆菌间的穿梭载体，将groEL启动子和荧光素酶基因lux+插入到构建的穿梭载体中得到穿梭表达载体，并将该载体转化大肠杆菌诱导荧光素酶基因的表达。

Transcriptional activity of ACL recombinants normalized by Renilla was significant difference with pGL3-Basic expect for construct -27 and -15 (P＜0.01). Construct -919 contains highest activity. 3 times reduction of transcriptional activity from-919 to -679bp indicated that negative regulation factors located probably in this region. The activity started on construct -73 suggested the basal promoter activity was located within the -73 to +77bp region; Transcriptional activity of IDHβrecombinants was not significantly different between the recombinant -58 and pGL3-Basic. The activity started on construct -82, decreasing with the length of the fragment up to -164 in despite of a bit of fluctuation, and kept increasing from construct -164 up to -279. Thus, the basal promoter activity was located within the -82 to +16bp region, whereas the upstream 197bp conferred maximal transcriptional activity.

采用5'端缺失策略，结合启动子预测结果，分别构建了8个ACL基因和19个IDH3β基因启动子区重组子，将其转染猪PK15细胞系，并利用荧光素酶双报告基因系统检测了它们的活性，结果表明：在所构建的猪ACL基因5侧翼序列的8个重组子中，除pGL-ACL27和pGL-ACL15外，其它重组子的荧光素酶活性与阴性对照均达到极显著水平(P＜0.01)，表明它们均具有启动子活性，pGL-ACL919活性最强，到pGL-ACL679活性下降了将近3倍，说明从-919bp到-679bp区域可能存在负的调控位点，从pGL-ACL621到pGL-ACL73活性有小幅度下降，到pGL-ACL27活性降到与阴性对照水平无显著差异，表明维持该启动子的基本活性区域位于-73bp到+77bp之间；IDH3β基因启动子活性开始于pGL-IB82，然后虽然有些波动，但是活性一直下降直到-164bp处，之后活性又开始升高，直到-279bp处活性达到最高，这些表明维持该启动子的基本活性区域位于-82bp到+16bp之间，从-82到-279之间的179bp区域具有最高的启动子活性。

The absorption and distribution of chromium were studied in ryeusing nutrient culture technique and pot experiment.

采用不同浓度K2CrO4(0，0.4，0.8和1.2 mmol/L)的Hoagland营养液处理黑麦幼苗，测定铬在黑麦体内的亚细胞分布、铬化学形态及不同部位的积累。

By analyzing theory foundation of mathematical morphology in the digital image processing, researching morphology arithmetic of the binary Image, discussing two basic forms for the least structure element: dilation and erosion.

通过分析数学形态学在图像中的理论基础，研究二值图像的形态分析算法，探讨最小结构元素的两种基本形态：膨胀和腐蚀；分析了数学形态学复杂算法的基本原理，把数学形态学的部分并行处理理念引入到家实际应用中。