荧光素

Second Municipal People's Hospital of Guangzhou, Guangzhou 510150 Objective To study the technique and diagnostic value of fluorescence insitu hybridization in chromosome abnormality for prenatal diagnosis. Methods Amniocenteses were performed in 34 pregnant women of 16～23 gestational weeks with indications for prenatal diagnosis. The amniotic fluid samples were cultured in Chang's medium.

对34例孕16～23周有产前诊断指征者经腹部抽取羊水，用Chang培养液传代培养，常规制备羊水细胞分裂中期染色体，应用生物素及地高辛标记的人类全着丝粒探针和X、Y、13、21、18号染色体α-着丝粒探针及染色体全涂染探针，与培养的羊水细胞分裂中期染色体DNA进行原位杂交，杂交后用荧光显微镜观察玻片并摄像。

Methods Amniocenteses were performed in 34 pregnant women of 16～23 gestational weeks with indications for prenatal diagnosis. The amniotic fluid samples were cultured in Chang's medium. The metaphase chromosomes were hybridized insitu with the human centromere probes,α-satellites DNA probes of X, Y,13, 21, 18 chromosomes and all primer chromosome probes. These probes were conjugated by Biotin and Dioxin then. The treated slides were examined and taken photos under the fluoromicroscope.

对34例孕16～23周有产前诊断指征者经腹部抽取羊水，用Chang培养液传代培养，常规制备羊水细胞分裂中期染色体，应用生物素及地高辛标记的人类全着丝粒探针和X、Y、13、21、18号染色体α-着丝粒探针及染色体全涂染探针，与培养的羊水细胞分裂中期染色体DNA进行原位杂交，杂交后用荧光显微镜观察玻片并摄像。

Under fluorescence microscope, cells treated with curcumin showed shrunk or distorted nucleus with fluorescence conglomerated.

姜黄素作用后，Hoechst染色显示A549/DDP细胞核缩小变形，致密浓染荧光成团块分布。

Emodin induced the nuclei of K562 with anomalistic and fragmentized for 96 hours. Agarose gel electrophoresis of DNA revealed a typical DNA ladder map of apoptosis.

大黄素作用96h后，荧光显微镜下可见细胞核不规则，核碎裂呈新月形改变，DNA琼脂糖凝胶电泳显示，基因组DNA显现典型的凋亡细胞的梯状图谱。

Take SYBR Green I as the intercalating fluorescence dye in Real-time PCR,the overexpression of WIPK and SIPK,which were the key genes of MAPK cascades,and the resistant gene PR1 in tobacco treated with copper and magnesium solution were studied.

采用SYBR GreenⅠ作为荧光染料的Real-time PCR方法，明确了铜、镁素能调控烟株体内蛋白激酶级联信号转导途径的关键基因WIPK、SIPK及抗病信号通路末端PR1基因的过量表达，进一步揭示了矿质营养调控烟草对病毒抗病性的分子机制。

Methods Human luteinized granulosa cells were isolated from follicular fluid obtained during oocyte retrieval of IVF-ET program and were cultured with M199 media plus various concentration of follicle stimulating hormone (FSH, 0% 0.5% 5% 10% 25% 50) or human chorionic gonadotropin(hCG, 0% 5% 10% 50% 75% 100IU/ml). The media were collected and PAPP-A in the media were measured by time-resolved fluoroimmunoassay.

将来自体外受精—胚胎移植的卵泡黄素化颗粒细胞分离纯化后，利用不同剂量促卵泡激素(FSH，0、0.5、5、10、25、50)或人绒毛膜促性腺激素(hCG，0、5、10、50、75、100IU／ml)刺激体外培养的颗粒细胞，收集培养液，采用时间荧光免疫分析法测定培养液中PAPP-A的量。

Objective:To establish a real-time quantitative reverse transcription polymerase chain reaction method for examining the expression of sialic acid-binding immunoglobulin-like lectin-one (Siglec-1)gene in the peripheral blood mononuclear cells in patients with coronary heart disease,so as to explore the relationship between Siglec-1 mRNA expression and the development/progression of CHD.

目的：建立检测人Siglec-1(sialic acid-binding immunoglobulin-like lectins，唾液酸结合的免疫球蛋白样凝集素1，CD169)mRNA 含量的实时荧光定量RT-PCR 的方法，并用来测定冠心病患者外周血单个核细胞中Siglec-1的基因表达水平，探讨Siglec-1基因表达水平与冠状动脉粥样硬化发生发展的关系。

Fluorescence spectra and UV absorption spectra of aesculin were studied.

研究了秦皮甲素的荧光光谱和紫外吸收光谱。

Methods The sample was separated with a C18 column (150mm×4.6mm, 5μm) at 30℃ with the mobile phase of methanol and water (74:26); A fluorescence detector was used (λex=230nm λem=460nm); The floss rate was 1mlmin^(-1) Results The retention time of aesculin was(3.620±0.05)min; A good linear relationship of aesculin in the plasma samples at the concentration between 0.1mgL^(-1) and 100mgL^(-1) was observed (r=0.997); The mean absolute recovery was 74.0%~82.6% and the lowest detective limit was 0.05ng; Both the intra-day and inter-day precisions were less than 9.0%.

以C18柱(150mm×4.6mm，5μm)分离；流动相为甲醇－水(76：24)；流速：1mlmin^(-1)；柱温：30℃；荧光检测器，激发波长230nm，发射波长460nm：结果秦皮甲素的保留时间为(3.620±0.05)min；血药浓度在0.1~100mgL^(-1)范围内线关系良好(r=0.997)；绝对回收率为74.0%-82.6%，日内、日间精密度均＜9.0%，最低检测限为0.05ng。

D. N big mouse model was successfully made by adopting single side kidney cutting with induction, then by 24 week continuos observation on the change of various values of kidney and function, through testing of immunity florescence, electro-scope and optical scope, what we can see from model group is that typical kidney small cell got hardened by knot, and discarded, and dead, also small tube withered, small cell of on base film got thick, cell capsule wall was fibred, and base film got double layered.

实验研究采用单侧肾切除加以链脲佐菌素诱导，成功地复制出糖尿病肾病大鼠模型，对其肾脏结构和功能的改变各项指标，连续进行24周动态观察，并进行免疫荧光、电镜、光镜检测，结果表明：糖尿病肾病模型组可见典型的肾小球节段硬化、废弃、坏死，有大量蛋白管型，肾小球管糖元空泡沉积及脂肪滴，肾小管萎缩，肾小球基底膜明显增厚，球囊壁纤维化，基底膜双层化，有一例结核性肉芽组织及钙化等病理变化。

Lugalbanda was a god and shepherd king of Uruk where he was worshipped for over a thousand years.

Lugalbanda 是神和被崇拜了一千年多 Uruk古埃及喜克索王朝国王。

I am coming just now,' and went on perfuming himself with Hunut, then he came and sat.

我来只是现在，'歼灭战perfuming自己与胡努特，那麼，他来到和SAT 。