荧光素

The difference of fluorescence quenching between light-adapted and dark-adapted bacteriorhodopsin showed this quencher can be used in the study of conformational change of Biomembrane proteins.

竹红菌乙素对明暗适应菌紫质荧光猝灭的差别，说明这种猝灭剂可用于生物膜体系的蛋白质构象变化的研究。

The oxidation of dihydrorhodamine 123 to fluorescent rhodamine 123 was also inhibited by vanillin.

氧化dihydrorhodamine123至123罗丹明荧光也抑制香兰素。

Changes of endogenous ZT and GA3 in stems and leaves of spring shoots of citrus Satsuma on the stock of Poncirus trifoliuta during the period o flower bud formation were determined by HPLC and TLC respectively.

用高效液相色谱法和薄层荧光色谱法分别测定了宫川温州蜜柑花芽分化过程中内源玉米素和赤霉酸(GA3)的含量变化。

Keywords Capillary electrophoresis;on-line sweeping concentration;hollow fiber-based liquid-phase microextraction;fluorescence spectroscopy;interaction;serum albumin;Strychnos alkaloids;fangchinoline;tetrandri

毛细管电泳；在线推扫富集；中空纤维液相微萃取；荧光光谱法；相互作用；血清白蛋白；马钱子碱；防己诺林碱；粉防己碱；槲皮素；卡马西平；甲基多巴；左旋多巴

Relationship between emodin concentration and intracellular [Ca2＋]I as well as kinetics of [Ca2＋]I in guinea pig taenia coli cells were studied.

利用Fluo－3荧光探针检测细胞内自由Ca2＋浓度([Ca2＋]I)，研究了大黄素升高豚鼠结肠带细胞[Ca2＋]I的量―效关系和动态变化特征，及GDP和胞外Ca2＋浓度对其的影响。

Objective To evaluate the therapeutic effect of azithromycin on infant toxoplasmosis patients by using fluorescence quantitative polymerase chain reaction.

目的探讨荧光定量聚合酶链反应用于评价阿奇霉素治疗儿童弓形虫病的效果。

Methods Radio-immunological and fluorescence methods were used to determine the amount of insulin secretion as well as content of intracellular Ca++ and cAMP by the rat islet cells incubated with amylin in different concentrations.

用放免和荧光方法分别检测经不同浓度胰淀素温育的大鼠胰岛细胞的胰岛素分泌量、细胞内Ca++及cAMP含量。

The role of LM609 in bone marrow micrometastasis in athymic mice was detected by quantitative real-time RT-PCR.

实时荧光定量RT-PCR检测抗整合素αvβ3抗体LM609对裸鼠乳腺癌骨髓微转移模型的影响。

However, it is not known how orthodontic force influences the function of cementoblast and what is the relationship between the function change of cementoblast with root resorption. So we studied the effect of mechanical stimuli on root resoption by examining the expression OPG and RANKL and exploring the related signal transduction pathway in cementoblast. Methods: Cementoblasts OCCM30 were cultured in DMEM with 10% FBS for 48 hours and starved in DMEM with no FBS for 24 hours, then subjected to mechanical strain by four-point bending system with tension and compression stress at 2000μstrain at 0.5Hz frequency. The flow cytometry was used to examine the cell cycle and proliferation activity after 3h, 6h, 12h, 24h loading respectively. OPG and RANKL mRNA were analysed with real time quantitive RT-PCR after the same loading period as FCM.

本课题利用四点弯曲力学加载系统，采用流式细胞术、实时荧光定量PCR、Western免疫印迹等方法，研究体外培养的成牙骨质细胞株OCCM-30在不同力学环境中增殖活性的变化，以及其表达骨保护素和破骨细胞分化因子的mRNA的变化，探讨应力刺激对成牙骨质细胞表达破骨细胞分化调控蛋白的影响，并通过对应力刺激下成牙骨质细胞分裂原活化蛋白激酶家族中的ERK1/2、P38MAPK活性的变化及其与OPG、RANKL基因表达变化关系的研究，对力学刺激调节破骨细胞分化调控蛋白的相关上游信号转导通路进行初步探讨。

In the buffer solution of HAc-NaAc at pH 3.5, Cr reacts with chrysin by molar ratio 1:3, and forms a highly fluorescent active complex in the presence of some methanol. The maximum excitation and emission wavelengths of the complex are 260 nm and 429 nm respectively.

在含一定量甲醇的pH=3.5的HAc-NaAc缓冲溶液中，铬与白杨素能形成1：3的二元荧光配合物，其最大激发波长和发射波长分别为260 nm和429 nm。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐