荧光素

Methods:(1)Immunofluorescence and electron microscopic autoradiography were used to detect the expression and distribution of somatostatin receptor 2 subtype in NCI-H446 cells.

方法①应用免疫荧光技术及电镜放射自显影技术检测体外培养的小细胞肺癌NCI-H446细胞中生长抑素受体亚型2的分布与表达特性。

Fluorescence in situ hybridization was performed to detect the existence of HBV DNA in the blastomere by using the biotinylated probe.

废弃的胚胎卵裂球经激光打孔，活检2～4个卵裂球细胞，利用生物素标记的探针，应用荧光原位杂交技术检测HBV感染情况。

The analysis of sister chromatid exchange frequencies is a useful tool for the study of damages in genetic material of human beings induced by enviromental mutagens and carcinogens.

用荧光加Giemsa术区分姐妹染色单体，分析了13名中年男子外周血淋巴细胞的SCE，比较吸烟者和不吸烟者"自发"的和用丝裂霉素诱发的SCE频率变化。发现吸烟者的SCE频率(8.33±1.08)显著地高于不吸烟者(4.41±0.72)，p.001。

Methods: The inhibitation of HL-60 cells by Rapamycin was measured by MTT assay. Morphic change of the apoptosis of HL-60 cells was observed by inverted microscope and light microscope, trapezoid straps by DNA agarose gel electrophoresis, and apoptosis rate by flow cytometry. Western-blot analysis was performed to detect the expression of Bax and Bcl-2 proteins after rapamycin treatment.

用MTT比色法测定雷帕霉素对HL-60细胞增殖的影响；用倒置显微镜和荧光显微镜观测细胞凋亡的形态学改变；DNA琼脂糖凝胶电泳观测凋亡的梯形条带；流式细胞仪检测细胞凋亡率；Western-blot检测HL-60细胞经雷帕霉素处理后，Bax、Bcl-2蛋白的表达情况。

As the first attempt, it was designed to reveal the general pattern of lectin receptor distribution on the surface of female cells, namely egg cells, central cells and synergids of Torenia fournieri and, especially, toensure the possible effects of cell isolation procedure on the distribution of lectin receptors by four different systems.

在烟草活体的中央细胞及卵细胞表面发现了伴刀豆凝集素受体的极性分布或者称之为&镶嵌性分布&。在大部分的中央细胞膜表面，该凝集素受体主要分布在靠近核的部位，而在相对侧的荧光要弱或缺失。

Flow cytometric analysis showed that after mock infection, Ad5null infection, and Ad5hSeFT infection, the mean fluorescence intensity values for the binding of Ulex europaeus Ⅰ lectin to NIH3T3 cells were 2. 3±0.6, 2. 1±1. 0, and 36. 5±5. 9, respectively; MFI values for the binding of Griffonia simplicifolia isolectin B4 (GS-IB4) lectin to NIH3T3 cells were 167±23, 170±19, and 100±14, respectively; MFI values for the binding of human natural IgG and IgM antibodies to NIH3T3 cells were 31±3, 32±4, and 22 ±4, respectively.

流式细胞分析结果表明，小鼠NIH3T3细胞及分别被Ad5null和Ad5hSeFT转导的NIH3T3细胞，UEA-1凝集素结合细胞的平均荧光强度值分别为2.3±0.6、2.1±1.0和36.5±5.9；GS-〓凝集素结合细胞的MIF值分别为167±23、170±19和100±14：人天然IgG和IgM抗体结合细胞的MFI值分别为31±3、32±4和22±4。

The n-butanel separated solution was decolored on Al2O3 column, low pressure condensed, filtered, dried, dissolved and crystaled.

以薄层层析色谱上葛根素荧光成分的消失作为提取的终点，结果表明：葛根素收得率为1.41 %，纯度为97 %。

The distribution of fibronectin in human oocyte and 2-cell to morulae embryos was also investigated, and the possible roles of fibronectin in fertilization, the development of early embryo and implantation were also discussed.

应用免疫组织化学方法和图像分析技术研究整合素β_3、FN和ER、PR在输卵管粘膜内的表达和变化；采用间接免疫荧光技术对FN在人卵母细胞和早期胚胎内的表达分布进行观察；光镜观察输卵管粘膜上皮细胞的形态学变化。结果：1。整合素β_3在人正常输卵管粘膜上皮纤毛细胞的胞浆内表达。

However, when the pH value of the PBS buffer solution was adjusted to a value (such as 11) higher than the isoelectric point of avidin, the fluorescence spectrum of the CdTe-avidin solution was shown to be the same as that obtained with CdTe nanocrystals only.

实验证明CdTe量子点－亲和素体系的光谱在pH值从7变到9的范围内几乎没有什么变化，而当体系pH值超过亲和素的等电点时，体系的荧光光谱则会变成类似于单独量子点的谱图。

Near-infrared fluorescence of Yb was further enhanced while the visible fluorescence from Yb-Calcein was quenched when second ligand, such as tributyl phosphate, 1,10-Phenanthroline and trioctyl phosphine oxide, was added.

第二配体TBP，Phen，TOPO的加入，可进一步提高Yb~(3+)-钙黄绿素体系的近红外发光效率，但同时也会猝灭Yb~(3+)-钙黄绿素体系的可见区荧光。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐