荧光素

The leaf structure, content and the storage location of aloin in the leaves of six species of Aloe L. were studied by means of semi_thin section, high performance liquid chromatography and fluorescent microscope.

应用半薄切片法、高效液相色谱法和荧光显微镜研究了 6种芦荟叶的结构、芦荟素的含量和储藏芦荟素的组织。

Methods The inter-leukin2activity was observed by MTT colormietric assay,the interleukin2receptor expression of lymphocyte mem-brane was examined by immunofluoresence technique and lymphocyte blastogenesis in peripheral blood was measured by morphologic observation.

通过体外实验，运用MTT比色法、免疫荧光技术、淋巴细胞转化试验等实验方法，检测白细胞介素2活性、淋巴细胞膜白细胞介素2受体含量及外周血淋巴细胞转化功能。

Chapter 4:The interactions between bergenin,esculin,gallic acid and caffeic acidwith HSA in AOT/isooctane/water microemulsions were characterized for the firsttime using fluorescence spectroscopy in combination with UV absorptionspectroscopy,FT-IR spectroscopy,CD spectroscopy and dynamic light scattering technique.

第四章：利用荧光光谱法、紫外-可见吸收光谱法、圆二色性光谱法、傅里叶变换红外光谱法和动态激光散射技术首次系统研究了在AOT/异辛烷/水构成的反相微乳介质中岩白菜素、秦皮甲素、没食子酸和咖啡酸与HSA之间的相互作用。

Ulex Europeus was used as a probe to study the expression of α L Fucose in mouse uterus during early pregnancy,pseudopregnancy and estrous cycle.

应用免疫荧光技术以生物素化的荆豆素-为探针，检测其结合位点α-L-岩藻糖在小鼠妊娠 1～ 8d、假孕 1～ 6 d及发情周期子宫中的表达情况，并以摘除卵巢的小鼠为模型观察雌激素、孕酮对小鼠子宫α-L-岩藻糖表达的影响。

Liver function and pathological changes of hepatic tissue were examined.

采用ANIT灌胃制备大鼠急性肝内胆汁淤积病理模型，共32只大鼠，设正常组、正常＋大黄素组、模型组和模型＋大黄素组，观察各组实验动物肝功能各项生化指标和肝组织病理学改变，并采用实时荧光定量RT-PCR检测肝细胞膜转运蛋白基因多药耐药蛋白1a（mdr1a）、多药耐药蛋白1b（mdr1b）和多药耐药蛋白2（mdr2）mRNA水平的表达，Western blot方法检测肝脏P-糖蛋白的变化。

When adding different concentrations of skatole into those three enzymes, the

通过实验我们得知粪臭素对三种消化酶的内源荧光均有很强的猝灭作用，根据Stern—Volmer方程作粪臭素对三种消化酶猝灭的F_0／F～曲线，可得出

First we studied the effect of skatole on activity of a-amylase pepsin and trypsin, then we studied the effect of skatole on structure of three important digestive enzymes by UV spectra and fluorescence spectra.

本文首先研究了粪臭素对α-淀粉酶、胃蛋白酶及胰蛋白酶活性的影响，接着通过紫外光谱法和荧光光谱法进一步研究了粪臭素对这三种重要消化酶结构的影响。

It included the development of the trilinear decomposition algorithms as well as the applications of these algorithms to different fields of analytical chemistry, in particular, to three-way fluospectroscopy (excitation-emission matrix fluospectroscopy)In Chapter 2, a method of simultaneous determination of two main components of the Chinese traditional medicine cortex fraxini was introduced.

第二章利用激发-发射荧光分析方法分析了中药秦皮粉末萃取液，结合交替三线性分解算法，在无须预分离的情况下，直接对秦皮实际萃取液中秦皮甲素和秦皮乙素这两种主要成分同时定性定量分析，方法允许在其他组分的干扰共存。

METHODS The cellular up take of Hypericin extractwas investigated by detecting endocellular fluorescence, and the antitumoral effect of Hypericin extract was assessed by light microscopy, MTT assay and DNA ladder gel electrophoresis.

通过测定胞内荧光强度来确定细胞对金丝桃素的吸收，用显微镜观察、MTT法和DNA电泳分析法来研究金丝桃素对细胞的生长抑制作用。

Methods The conditions of solvent extracting active ingredient from pueraria lobata were optimized,and evaluating indIcators were contents of puerarin,lead and mercury, which were determined by high performance liquid chromatography and atomic fluorescence spectrometry.

方法采用高效液相色谱法和原子荧光光谱法测定葛根提取液中葛根素和铅、汞元素的含量，以葛根素和铅、汞元素的含量为评价指标，对影响溶剂回流提取葛根有效成分的条件进行优化。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。