荧光素

IFPs express well in mammalian cells and mice and spontaneously incorporate biliverdin, which is ubiquitous as the initial intermediate in heme catabolism but has negligible fluorescence by itself.

红外荧光蛋白在哺乳动物细胞和小鼠中表达良好，并且能够自发结合到胆绿素中，胆绿素作为初始中间体在血红素分解代谢中普遍存在，又含有自身可以忽略的荧光。

Purpose To study the characteristics of fluorescence of emodin and its effects on fluorescence analysis in smooth muscle cell.

目的 观察大黄素的荧光光谱特性，以探讨其在平滑肌细胞中干预后对荧光分析的影响。

Abstracts The effects of fixation with paraformaldehyde or glutaraldehyde and preservation with liquid nitrogen on cell size, density, phycoerythrin fluorescence value (FL2) and chlorophyll fluorescence (FL3) of picoplanton cells were studied by using flow cytometry.

摘要以流式细胞计为海洋微微型浮游生物的分析工具，研究了醛类固定剂的固定、液氮贮存对于微微型浮游生物的细胞大小、密度、藻红素荧光值(FL2)和叶绿素荧光值(FL3)的影响。

The influence of acidity and concentration on fluorescence and absorption spectra were investigated. Using quinine bisulphate as a reference, fluorescence quantum yield of aesculetin in the condition of pH=9.32 was determined to be 0.13 at excitation wavelength 365nm.

考察了酸度与浓度对其荧光性质和吸光性质的影响，以硫酸奎宁为参比，测得秦皮乙素水溶液在pH=9.32，激发波长365nm条件下的荧光量子产率为0.13。

The accumulation of fluorogen may play a major role in the yellowing and increasing fluorescence of the lens with age.

荧光发生素在晶状体中积累，可能主要参与老化过程中晶状体变黄和荧光色素增加。

①Rat MSC and VSMC were cultured and identified, respectively. MSC were labeled with DAPI firstly, and then co-cultivated with VSMC. The changes of morphology and ultrastructure of co-cultured cells were observed. Immunfluorescence analysis was performed by using monoclonal antibodies against specific antigen.②We established the regulatable system in two steps: a stable MSC line expressing rtTA has been constructed and characterized firstly by transfected with pUHD 17-1hyg and then selected by hygromycin B; in a second step, this line was used for trandfer the AT2R gene to MSC to get the well establishing double stable MSC lines;③The expression of AT2R regulated by doxycycline was evaluated by western blot;④The MSCs were transduced into rat carotid arteries with regulatable AT2R gene after the establishment of rat carotid balloon injury restenosis model. The intimal/medial area ratio were measured by digital analysis system.

研究方法：(1)密度梯度离心法及胶原酶消化法分别培养原代大鼠MSC及VSMC，细胞共培养并行免疫荧光化学染色和透射电镜观察超微结构；(2)组成受Dox调控的哺乳动物表达系统的四种成分的转化、扩增及提纯并酶切鉴定；(3)采用常规分子生物学方法连续两个回合转染体外培养的MSC，并分别采用发光计检测不同细胞克隆萤光素酶活性改变以及RT-PCR方法检测AT2R目的基因mRNA表达情况，根据各个细胞克隆受Dox调控表达的程度，选择低背景、高诱导表达AT2R的细胞系，作为双重稳定MSC细胞系；蛋白免疫印迹法观察该细胞系在Dox调控下AT2R表达的时相性、持续性及在不同浓度Dox调控下的表达情况；(4)建立大鼠颈动脉球囊损伤动物模型，将双重稳定MSC在术中导入血管，分别于14 d、28 d进行病理切片，检测可调控AT2R对新生内膜增生的影响；采用RT-PCR免疫组织化学免疫荧光等技术观察AT2R基因在新生内膜中的表达以及细胞外基质成分表达的改变，TUNEL法检测血管组织中细胞凋亡的变化情况。

The change of agglutinating activity in different temperature and pH indicated that PML had no hemagglutinating activity at 80℃. The effect of chemical modification of amino acid residues upon hemagglutination was investigated. There was a definite decrease of fluorescence intensity which corresponds to the oxidation of tryptophan.

研究了不同温度、pH和变性剂对PML凝血活性的影响和荧光光谱的变化，当温度达80℃时，活性完全丧失；pH为4—9对活性影响不大，pH低于2和pH高于12时，大黑花芸豆凝集素的凝血活性大部分丧失；高温和强碱对荧光光谱有较大影响，表明PML对高温，强碱的耐受性不强。

First, at the condition of pH≈3, the quantity of aesculin was determined by choosing excitation wavelength 335nm and emission wavelength 455nm.

首先在pH≈3时，选择荧光激发波长335nm，荧光发射波长455nm，测定秦皮甲素的含量。

Wheares under buffer pH5.2, there is no strong static electricity and instead being a longer wavelength movement; SDS\'s participation just restricts the relative movement of heme\'s ethylene; a new fluorescent peak forms and the reduction of enzymatic activity is due to the packing of micella\'s formation.

强变性的阴离子表活剂SDS与带净正电荷和弱负电荷的SBP(处于pH2.6和4.2缓冲液中)有强烈的静电力作用，酶活力丧失，二级结构变化，荧光强度增加，特征Soret发生蓝移，卟啉环的π-π*共轭的分裂能增大；而当SBP带净负电荷(pH5.2)时，没有静电吸引力，Soret带发生红移；SDS的引入只是限制了血红素上乙烯取代基的运动，使其与卟啉环共轭：极性环境的变化导致产生新的荧光峰；酶活力因胶团包裹作用而有下降。

The assay, which was based on primers and TaqMan-MGB probes selected from highly conserved regions of the hemagglutinin gene of avain influenza virus subtype H5, was optimized in a reaction system and a condition to improve the sensitivity, specificity and accuracy. In addition, colone technology was used to develop a quantitative PCR format with virus copies amount.

针对H5亚型禽流感病毒血凝素基因保守区域设计特异性引物与TaqMan-MGB荧光探针，筛选并优化荧光定量RT-PCR反应体系与反应条件，用以提高方法的特异性、敏感性与准确性；并通过体外克隆技术建立病毒基因拷贝数进行定量分析。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。