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荧光素

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This diad or model compound (FL4) is adsorbed on TiO_2 colloid through the phenolic function of the fluorescein moiety.

发现荧光素蒽醌甲酯与荧光素丁酯均可通过酚羟基较好地吸附在TiO_2胶体上。

The complex has a maxmium absorption at 480 nm with 9 nm of red shift compared to fluorescein itself.

研究了在生理条件下,荧光素与牛血清白蛋白相互作用形成复合物,最大吸收峰的波长480nm ,比荧光素红移9nm。

Forty-eight hours after transfection, cells were harvested to determine luciferase activity by illuminometer, In the presence of RU486 a 40-fold maximum activation of the luciferase reporter gene was observed in cultured cells, whereas in the absence of RU486, no significant activation was observed.

加入诱导剂RU486后,可以诱导表达荧光素酶,并在一定范围内两者呈正比,最高可以实现荧光素酶的40余倍的表达,而没有RU486时,几乎没有报告基因的表达,表明RU486诱导调控载体构建成功,可实现对目的基因的表达时间和表达水平的精确调控,为进一步的基因调控研究和基因治疗提供了良好的工具。

Forty-eight hours after transfection, cells were harvested to determine luciferase activity by illuminometer. In the presence of RU486 a 40-fold maximum activation of the luciferase reporter gene was observed in cultured cells, whereas in the absence of RU486, no significant activation was observed. There was a positive correlation between the luciferase activation and RU486 concentration in a definite range. The results showed that the RU486-inducible regulatory vector was successfully constructed, which can be used to regulate the expression level of genes of interest in appropriate time by the inducer RU486. This inducible expression vector provides a platform for the research of gene regulation and gene therapy.

加入诱导剂RU486后,可以诱导表达荧光素酶,并在一定范围内两者呈正比,最高可以实现荧光素酶的40余倍的表达,而没有RU486时,几乎没有报告基因的表达,表明RU486诱导调控载体构建成功,可实现对目的基因的表达时间和表达水平的精确调控,为进一步的基因调控研究和和基因治疗提供了良好的工具。

Since the different kind and different function and character, what have been done in this paper would shed a light on the study of the firefly luciferase.

由于不同种类的荧光素酶的性质有一定的差异,所以本研究结果为更好的了解和利用萤火虫荧光素酶提供了一些有用的资料。

In order to understand the biosynthesis and origin of the luciferin,the paper will summarize both historical and recent data on the structure?property and the biosy...

为了更清楚的认识荧光素在体内的生物合成与起源问题,本文对几种主要荧光素的结构性质和有关其生物合成研究的最新报道进行综述。

An enzyme present in the cells of bioluminescent organisms that catalyzes the oxidation of luciferin.

荧光素酶存在于生物性发光组织的细胞中,催化荧光素氧化的酶

In this study, semi-quantitative RT-PCR, ABC immunohistochemistry assay, flow cytometry analysis and luciferase/luciferin assay were used to study the expression and function of CD39 in seven hematopoietic cell lines.

采用半定量RT-PCR、ABC免疫酶标和流式细胞术以及荧光素/荧光素酶法,研究了七个血源细胞系中CD39的表达和功能。

In this paper, we studied the relationshipbetween the concentration, acidity, temperature, heavy metalic ions and the fluorescencequenching of fluorescein. The result showed that when the concentration was over 1. 55 ×10-5 mol/L, the fluorescence quenching strengthened with the increase of f...

结果表明:荧光素浓度大于1.55×10-5mol·L-1时,随着浓度增大,荧光猝灭作用增强;酸度对荧光猝灭作用有明显作用,PH值小于8.5时,随着PH值升高而减弱,PH值大于8.5时,随着PH值升高而增强;温度对荧光猝灭作用呈线性关系,温度升高,荧光猝灭作用减弱;重金属离子对荧光猝灭有显著的作用,是影响眼底血管造影质量的重要因素。

The difference between the test groups and the control group was statistically significant. It could be deduced that all the insecticides tested in this experiment damaged the spiders′ intestinal mucosa, compromising its function as a barrier and letting large molecules such as FTIC-D more easily penetrate. Among the insecticides, pyrethrin and Bt preparation are bio-insecticides, and the FITC-D concentrations of the spiders exposed to these two bio-insecticides were more than those of the spiders exposed to the chemical insecticides imidacloprid and dipterex. The difference between the bio-insecticide groups and the imidacloprid group was significant, but there was no significant difference between the bio-insecticide groups and the dipterex group.

结果表明:4种杀虫剂处理后拟环纹豹蛛血液中的荧光素浓度远高于对照组,各个处理组拟环纹豹蛛血液样本中的荧光素浓度均与对照组存在极显著差异,可见4种杀虫剂均将对拟环纹豹蛛肠黏膜的屏障功能造成损伤,导致大分子物质易于通过;其中1.5%除虫菊素水乳剂三保奇花和Bt制剂强杀处理后拟环纹豹蛛中肠黏膜的通透性高于仿烟碱类化学杀虫剂吡虫啉处理组,差异达到显著水平,但与有机磷杀虫剂敌百虫处理组差异不显著。

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