荧光素

Observation of section of filter membrane revealed a stratified cell growth of HPDLF and HGF 2 weeks after inoculation,and TEERs of HPDLF and HGF were 56.14±7.43 and 57.34±7.62Ω·cm~(-2) respectively.Being incubated for 30 minutes, less than 1%of fluorescein sodium reached the inferior filter membrane 2 week after inoculation.

结果：HPDLF及HGF接种1周后细胞基本汇合，到2周时细胞连接紧密且完整，细胞呈复层生长，跨膜电阻分别达56.14±7.4 3及57.34±7.62Ω·cm~(-2)，经荧光素钠孵育30分钟检测细胞旁渗漏小于1%。

So, there would be certain crosstalk between STAT3 pathway and WNT pathway. But no synergized activation of the two pathways was detected.

但利用荧光素酶活性分析方法发现STAT3对β-catenin转录激活活性没有明显的影响。

OR6c, a derivative of HuH7 cells, was transfected with the replicon including a luciferase reporter gene.

来源于HuH7细胞的OR6c被包含荧光素酶报道基因的复制子转染。

OR6c, a derivative of HuH7 cells, was transfected with the replicon including a luciferase reporter gene.

用荧光素酶标志基因复制子转染 HuH7细胞的衍生物 OR6c 。

In order to elucidate the regulation mechanism of RU5 region to BFV gene expression, BFV3026 provirus DNA was used to construct the plasmids containing different deletion of R region, which were cotransfected with luc report gene locatied behind the IP promoter to BL12 cells, and luciferase activities was assayed, confirming that U5 region could repress the initiation function of LTR as well as IP. The BFV structure genes with different deletion of R region were cloned closely behind to heterogenous CMV promoter, then transfected to 293T cells, RT activity was performed, testifying the R region was required for BFV pol gene expression, and also the function domain was identified within the 100n.t. sequence at the 5′end.

以牛泡沫病毒(Bovine foamy virus， BFV)中国株BFV3026原病毒DNA为材料，构建R区系列缺失质粒，通过对其转染细胞中RT水平及对缺失质粒与luc报告质粒共转染细胞中萤火虫荧光素酶活性的测定，确立U5区对于BFV3026两类启动子LTR和IP均具有负调控作用；同时将带有不同R区的BFV3026结构基因片段克隆于异源启动子CMV之下，通过对其转染细胞293T中RT酶活性的测定，确立R区对于病毒结构基因pol的表达具有一定的调节作用，并将其功能区域初步界定在R区5′端100bp内。

In this recombinant virus the Luc gene was under dual conntrol of the synthetic and XIV promotors.

在该重组杆状病毒株，萤火虫荧光素酶基因受到合成启动子和XIV启动子的双重调控。

Retinal vein occlusion ; retinal ; blood circulation time ; fundus oculi ; fluorescein angiography

视网膜静脉闭塞；视网膜；血液循环时间；眼底；荧光素血管造影术

Heating, ethanol precipitation, and ion-exchange chromatographies had been carried out to isolate the protein with specific stimulating activity from newborn calf liver, and [(3)H]thymidine deoxyribose/bromodeoxyuridine incorporation and carboxyfluorescein diacetate succinimidyl ester-based proliferation assay to determine the bioactivity in vitro and in vivo.

通过用加热、乙醇沉淀、离子交换层析等方法来分离新生小牛肝脏中含有特殊刺激活性的蛋白和用胸嘧啶脱氧核糖/溴脱氧尿苷联合羧基荧光素双乙酸钠琥珀酸酯为基础的扩增分析其体内和体外的生物活性。

METHODS: After anaesthetized, rabbits were cut at the skin for 2 cm long at the crossing of skull center and posterior canthus, skull was exposed and periosteum was separated, then a round skull window with diameter of 0.5 cm was drilled at 0.5 cm left or to sagittal suture and 0.5 cm posterior to coronal suture, after that, 35 g/L rose Bengal was slowly injected from ear-edge vein in dosage of 1 mL/kg by once.

兔麻醉后，沿颅骨的中央与眼后角垂直交叉处纵行切开皮肤约2 cm，暴露颅骨，刮离骨膜，在矢状缝左侧外0.5 cm与冠状缝后0.5 cm处，用钻头直径0.5 cm的颅钻钻透颅骨，造成颅骨圆形洞窗，随即由耳缘静脉按1 mL/kg一次性缓慢注入35g/L四氯四碘荧光素钠盐。约3 min后用冷光源(波长540 nm，功率140 lx)对准颅骨洞窗，连续照射8 min后，缝合皮肤切口。

In this paper, it was found that the weak ECL signal, generated from electrooxidation fluorescein on the surface of the carbon- printed electrode, was greatly enhanced by the chlortetracycline.

我们发现，碱性介质中的荧光素在碳质印刷电极表面的弱电化学发光信号可被金霉素所增敏。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐