荧光素

To test this hypothesis, we performed experiments using plasmids containing a luciferase reporter with amber, ochre and opal nonsense mutations within the luxB gene in Escherichia coli.

为验证此假设，我们用几个含有大肠杆菌荧光素酶报告基因的质粒进行了实验，这些质粒中的luxB基因分别包含有琥珀色、赭色和乳白色的无义突变。

Objective To observe the changes of Schirmer I test value, corneal fluorescein and rose Bengal staining, and morphological alterations of corned epithelium after lacrimal gland extirpation in rabbits.

目的 观察摘除泪腺后，兔泪液S1T值、角膜荧光素染色和虎红染色的变化及其角膜上皮超微结构的变化，评估兔干眼症模型的建立。

Methods: Sensitivities and specificities of traditional tests, corneal rose bengal/fluorescein staining tests and BUT test were e-valuated in 112 primary SS patients and 185 controls.

在112例PSS患者和185例对照者中，评估上述试验的敏感度和特异度及其主要影响因素。结果：Schirmer、角膜荧光素活体染色和BUT试验的特异度分别为43.8％、93.5％和31.4％，敏感度分别为91.7％、50.0％和87.5％。

To evaluate the characteristics of the rabbit dry eye model. Method The S1T value, corneal fluorescein and rose Bengal staining before and after lacrimal gland extirpation were graded. The cornea was taken for transmission electron microscopic examination.

摘除兔泪腺和Harder氏腺，比较泪腺摘除前后S1T、角膜荧光素染色和虎红染色分值的变化，并在透射电镜下观察角膜上皮形态学的变化。

Methods:In addition to the Schirmer Test for Tear Break-Up Time,Rose Bengal Staining and Fluorescein Staining were used.We tested the tear film mucous layer by CIC for a control group and for dry eye patients.

对45人90眼正常组和80人160眼实验组除采用泪液分泌实验I、泪膜破裂时间、虎红染色及荧光素染色计分等常用眼表泪功能检查外，重点采用CIC，通过观察结膜上皮细胞情况及杯状细胞数来了解正常眼、干眼的泪膜粘液层的情况。

The PCR products were examined by agarose gel electrophoresis. The target gene fragments were purified by gel extraction kit and ligated to cloning vector pMD18-T. The recombinant vectors were transformed into host strain E. coli K802 by lithium chloride method, screened and identified with PCR and restrictive enzymatic digestion. Their sequences were confirmed by DNA sequencing.(2) sTWEAK1 gene was subcloned into expression vector pProEx HTb and transformed into E. coli BL21. sTWEAK2 gene was subcloned into expression vector pMAL-C2x and transformed into E. coli TB1. The recombinant vectors were screened and identified with PCR and restrictive enzymatic digestion. The recombinant fusion proteins were induced to express with IPTG, detected by coomassie brilliant blue-stained SDS-polyacrylamide gel electrophoresis , and confirmed by Western blot analysis.(3) The sTWEAK1 fusion protein was purified with Ni-NTA Spin Kit.(4) The biological activity was assayed on transformed and tumor cells by microplate photometer after crystal violet or sulfur rodamine B staining.(5) The contents of IL-8 in the supernatant of 1990 cell cultures were determined by ELISA.(6) The morphological changes of the sensitive cells were observed by light and transmission electron microscopies.(7) The cell cycle and apoptotic rate were assayed by flow cytometry in 1990 and M85 cells.(8) The effect of fusion proteins on induction of NF-κB in 1990 and LOVO cells was detected with Dual-Luciferase Reporter Assay system.(9) The TWEAK gene was subcloned into Adeno-X Viral DNA with pShuttle vector and transfected into HEK293 cells by lipofectamine method.

(1)本研究用RT-PCR方法，从人组织细胞总RNA中扩增可溶性TWEAK胞外区（sTWEAK1和sTWEAK2）的cDNA序列及全长编码序列，用琼脂糖凝胶电泳分析PCR产物，胶回收目的基因片段，连接到pMD18-T克隆载体中，转化大肠杆菌K802，PCR和酶切筛选阳性克隆，全自动DNA测序验证序列；(2)sTWEAK1和sTWEAK2分别亚克隆到pProEx HTb和pMAL-C2x表达载体中，分别转化大肠杆菌BL21和TB1，PCR筛选和酶切鉴定，阳性克隆用IPTG诱导表达，表达产物用SDS-PAGE分析和Western blot验证融合蛋白；(3)用NTA-Ni Spin试剂盒初步分离纯化sTWEAK1融合蛋白；(4)用体外培养的肿瘤细胞和正常对表达产物进行活性检测，贴壁细胞用结晶紫染色法，悬浮细胞用磺酰罗丹明B染色法，酶标仪检测OD值；(5)敏感细胞用ELISA法检测细胞培养上清中IL-8的含量；(6)用光镜和电镜观察敏感细胞死亡和细胞凋亡情况；(7)用流式细胞仪分析表达产物对敏感细胞凋亡率和细胞周期的影响；(8)用双荧光素酶报告基因检测法，测定表达产物对敏感细胞NF-κB的影响；(9)用pShuttle穿梭质粒将TWEAK重组到腺病毒载体上，用脂质体转染法转染HEK293细胞，PCR鉴定重组质粒。

Cis-acting elements in 5\'-UTR generally have negative regulation on expression of downstream cistron.

本研究应用萤火虫荧光素酶和EGFP报告基因系统，分析上述7种MUHH致病突变对下游报告基因表达的影响。

The clinical characteristics and fundus fluorescence angiographic fundings of CSC are similar in both genders.

女性CSC的临床特征和眼底荧光素血管造影特点与男性相似。

Cell lines were co-tranfected with bi-plasmids and cultured at 1% O〓 condition, luciferase assay demonstrated that all of the expression systems with different domains could be hypoxia-activated. GAL-CAD achieved more than 100-fold induction in A549 cell and more than 40-fold in HepG2; expression level of the system markedly increased after addition of glycin arm, almost 10-fold enhancement in HepG2 cell. Because basic expression also increased, the induction fold by hypoxia decreased.

双质粒共转染不同细胞株后，在1％O〓环境下培养24h，荧光素酶活性检测结果提示含不同结构域的表达系统均有受缺氧激活的能力，其中GAL-CAD在A549中获得100倍以上的诱导，在HepG2中获得40倍以上的诱导；加入甘氨酸臂后该表达系统的转录活性大为增强，在HepG2细胞中增强了10倍以上，但是由于基础表达也有所增加，受缺氧诱导的倍数下降。

We used a reporter gene plasmid in which firefly luciferase expression is dependent on the HCV IRES.

我们使用了依赖HCV IRES表达萤火虫荧光素酶的报告基因质粒。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐