荧光素

The activities of fluorescein diacetate hydrolysis and soil enzymes of 12 high fertility paddy soils located in Taihu region were analyzed in this paper.

本文主要对太湖地区12个典型水稻土剖面的荧光素二乙酸酯水解酶活性及其它相关酶活性进行了分析。

An overall activity determination using the fluorescein diacetate hydrolysis method as described by Fontvieille et al.

在MBR和AS法的混合液和出水中对酶的活性进行考察，分成两个部分：1使用双乙酸荧光素水解法FDA研究酶活性（Fontvieille et al。

Methods Electrophoretic mobility shift assay and transient transfection assay were used in this study.

采用电泳迁移改变分析和荧光素酶报告基因瞬时转染分析。

To investigate the role of gene Gli3 in idiopathic congenital talipes equinovarus, we constructed the Gli3 luciferase reporter gene expression vectors to analyze the promoter activity of the rat gene Gli3. The regulatory element in the promoter region of the rat Gli3 was predicted using P-Match software and further verified by ChIP experiment.

为了探讨人类GLI3基因在单纯性马蹄内翻足(Idiopathic congenital talipes equinovarus， ICTEV)发生时所起的作用，文章构建了大鼠Gli3基因启动子区域荧光素酶报告基因表达载体来分析Gli3基因启动子的活性，并利用P-Match软件预测大鼠Gli3基因启动子区域可能的调控元件，应用ChIP实验加以验证。

Blood samples were obtained on 0,2,4,6 and 8 days after admission. Dynamic changes of immunological parameters including serum IgA,IgG,IgM,complements (C3,C4) levels were determined in each group at various intervals following trauma. Phagocytosis of monocytes was also measured by use of phagotest kits after blood cells were incubated with fluorescin isothiocyanate-labeled E.

分别在入院时及入院后2、4、6和8 d留取外周静脉血，检测血清免疫球蛋白(IgA、IgG、IgM)与补体(C3、C4)水平的动态变化，全血细胞与用异硫氰酸荧光素标记的大肠杆菌共孵育后检测单核细胞噬菌能力。

METHODS: A model of T cell activation and proliferation was established by stimulated the cells with Con A.T cells were treated with different concentrations of CPT. The expression of CD69, the early marker of CD3(superscript +) T cell activation, was measured by FACS. The proliferation index was determined by carboxyl fluorescin diacetate succinmidyl ester by flow cytometry. The cell-cycle distribution was analyzed by propidium iodide staining.

以ConA刺激小鼠T淋巴细胞，建立小鼠T淋巴细胞活化、增殖的模型，以不同浓度的CPT作用于该模型，流式细胞术检测T细胞早期活化标志CD69分子的表达；以活体染料羧基荧光素乙酰乙酸染色流式细胞术分析CPT在ConA刺激下小鼠淋巴细胞的增殖相关指数；以碘化丙啶染色流式细胞术分析细胞周期的分布情况。

Methods: The model of evaluating T cell proliferation stimulated with polyclonal activators, ConA was established by vital dye carboxyl fluorescin diacetate succinmidyl ester labeling technique. The effect of a specific inhibitor for p38 MAPK, SB253580, on T cell proliferation on different doses and in different time was estimated by flow cytometry. At the same time, the percentage of proliferating T cells and proliferation index were measured by CellQuest and SPSS10.0 for Windows softwares.

以活体染料羧基荧光素乙酰乙酸琥珀酰亚胺酯染色，建立了在多克隆刺激剂刀豆蛋白A刺激下评价小鼠T细胞增殖的模型，通过流式细胞术分析p38丝裂原活化蛋白激酶的特异性抑制剂SB203580在不同剂量、不同时间对T细胞增殖的作用，并应用CellQuest和SPSS10.0 for Windows软件分析增殖细胞各代所占比例和增殖指数。

Experiment showed that the separation efficiency in glossily channel for fluorescein sodium was 4.8 x105 per meter, under the electric field 200 V/cm and the separation length 3.5 cm, 17% higher than in rough channel.

用3.5cm分离长度的芯片毛细管电泳分离荧光素钠时，光滑通道的理论板数比粗糙通道提高17%，达到48万/米的理论板数。

The behaviors of supramolecular self-assembly of cabboxyl porphyrin-anthraquinone systems, carboxyl porphyrin zinc-copper systems, porphyrinatozinc-imidazolyl tailed porphyrinatomanganese systems, porphyrinatozinc-anthraquinone hybrids/imidazolyl tailed porphyrinatomanganese systems and porphyrinatozinc-fluorescein hybrids/imidazolyl tailed porphyrinatomanganese systems driven by hydrogen-bonding or coordination-bonding have been studied.

设计、合成了多种新型羧基卟啉、卟啉-蒽醌二元化合物和卟啉-荧光素二元化合物，研究了氢键或配位键驱动的羧基卟啉-蒽醌、羧基卟啉锌-羧基卟啉铜、锌卟啉-咪唑基尾式卟啉锰二元超分子，卟啉-蒽醌二元化合物与咪唑基尾式卟啉锰三元超分子的构建和光诱导电子转移性质。

Methods Twenty-nine eyes of 21 patients with DME underwent intravitreous injection with 4mg TA.

方法21例29眼DME患者玻璃体腔内注射4mgTA，治疗前后均行视力、眼压、裂隙灯、荧光素眼底血管造影和光学相干断层扫描检查。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐