荧光素

Firefly luciferase gene encodes an enzyme that catalyzes the adenosine triphos-phate-dependent oxidation of luciferin and thus produces luminescence.

来源于萤火虫的荧光素酶结构基因与花椰菜花叶病毒35SRNA启动子以及胭脂碱合成酶DNA3'末端结构相拼接，构成一融合基因。

In this study, it was observed that addition of vitamin C to oxidized low-density lipoprotein by cupric ions Cu(superscript 2+ could result in the formation of singlet oxygen (superscript 1O2 In experiments (superscript 1)O2 was detected by chemiluminescence method using a Cypridina luciferin analog, 2-methyl-6-(p-methoxyphenyl)-3, 7-dihydroimidazo [1, 2-a] pyrazin-3-one, as a selective and sensitive chemiluminescence probe.

以一种海萤荧光素类似物MCLA2-methyl-6-(p-methoxyphenyl)-3， 7-dihydroimidazo

Methods:The luciferase reporter plasmids of MDM2 P2 promoter containing different SNP309 were contructed,and cotransfected into CV-1 cells with AML1-ETO,AML1 and SP1 expression plasmid.The transactivities of luciferase were assayed by luminometer to determine the impact of AML1,AML1-ETO,and SP1 on MDM2 P2 promoter.

研究方法：构建含不同SNP309位点的MDM2 P2启动子的报告质粒，与AML1-ETO、AML1以及SP1的表达质粒单独或共转染CV-1细胞，测定荧光素酶的活性，分析AML1-ETO、AML1以及SP1对MDM2 P2启动子的影响。

Methods:The expressive plasmids of AML1b and AML1-ETO were transfected into CV-1 and 293 cells.The expression level of endogenous pig7 gene was detected by Realtime-PCR.The luciferase reporter plasmids containing pig7 enhancer/corresponding mutant sequences were constructed and co-transfected into CV-1 cells with expressive plasmids of AML1b and AML1-ETO.The transactivity of pig7 enhancer was assayed by luminometer.

研究方法：AML1b和AML7-ETO表达质粒分别转染及共转染CV-1和293细胞，用Realtime-PCR方法检测上述基因对两种细胞内源性pig7基因mRNA表达水平的影响；构建含AML1b结合位点的pig7基因增强子序列以及相对应的突变位点的荧光素酶报告基因质粒；将表达载体/突变载体与AML1b或AML1-ETO基因瞬时共转染CV-1细胞，分析AML1b、AML1-ETO对报告基因的转录调节作用。

We demonstrate that both the IL-5 mRNA expression and IL-5 protein production were stimulated by TSA and NaBu treatments. Chromatin immunoprecipitation assays showed that treatments of TSA and NaBu caused hyperacetylation of histones H3 and H4 at the IL-5 gene promoter in Jurkat cells, which consequently promoted the exogenous luciferase activity driven by this promoter.

TSA和丁酸钠能促进IL-5基因的mRNA和蛋白的表达水平，并增强该基因启动子所介导的外源荧光素酶报告基因的活性，这一增强作用与两种抑制剂介导的IL-5基因启动子上组蛋白H3和H4的高乙酰化有关。

Results:One hundred and eleven eyes were treated with PRP, 95 eyes were treated with mild-PRP. Visual acuity was increased by 1～3 lines in 82 eyes(35%), not changed in 94 eyes(46%) and decreased in 30 eyes(19%). On Fundus fluorescein angiography 3 months later, new vessels shrinked and retinal edema recovered in most cases, but in 10 eyes, new vessels and small nonperfusional areas still could be seen after the treatment, which was treated again.

结果：111只眼行广泛视网膜光凝术治疗，95只眼行亚广泛视网膜光凝术治疗。82只眼视力提高1～3行，占35%；94只眼视力无变化，占46%；30只眼视力下降，占19%。3个月后行荧光素眼底血管造影检查，新生血管大部分萎缩，视网膜水肿消退，有10只眼残留新生血管及小片状无灌注区，再次补充光凝。

Eye examination showed blepharospasm ball conjunctival hyperemia, edema, corneal punctate epithelial shedding, decreased corneal sensitivity, corneal fluorescein staining showed diffuse points, or flake coloring, the pupil to narrow as .24 hours, the symptoms and reduce the growth of new epithelium, 48 hour recovery, when the electro-optic stimulation of ophthalmia after symptom onset, can be partially points, 025% dicaine pain, point anti-inflammatory eye drops, hourly, pre-B anti-corneal infection .

眼部检查可见眼睑痉挛，球结膜充血、水肿、角膜上皮点状脱落，角膜知觉减退，角膜荧光素染色呈弥漫性点状或片状着色，瞳孔缩小。24小时内症状随着新生上皮的生长而减轻，48小时可痊愈，当电光性眼炎的刺激症状出现后，可局部点025％地卡因止疼，点消炎眼药水，每小时一次，预B防角膜感染。

ObjectiveTo probe into the characteristics that fundus fluorescein angiography was performed on hemodialysis patients having papilledema.

目的探讨行血液透析过程中出现视盘水肿患者的荧光素眼底血管造影的表现特征。

Methods 43 patients(49 eyes with 59 leakage spots) proved by fluorescein fundus angiography were subjected to OCT combined with fundus photochromy. The distance from spot?

对经荧光素眼底血管造影确诊的CSC43例（49眼59个渗漏点）行OCT联合眼底彩色照相检查，并测量渗漏点距黄斑中心凹的距离。

Methods The clinical data of 31 continuous patients (34 eyes) with PED diagnosed by ocular fundus photochromy, fundus fluorescein angiography and indocyanine green angiography from Oct, 2001 to Aug, 2004 were analyzed retrospectively.

方法回顾分析2001年10月至2004年8月经荧光素眼底血管造影确诊为PED的31例(34只眼)50岁以上连续性病例，眼底改变的特征，患者均进行眼底彩色照相、FFA及吲哚青绿血管造影检查。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。