荧光素

The interactions of the model compound (FL4) or diad composed of fluorescein and 2-methyl-anthraquinone and TiO_2 colloid have been examined by absorption, fluorescence spectroscopy and fluorescence lifetime.

测定了以荧光素为光敏剂的电子给体-受体二元化合物荧光素蒽醌甲酯及模型化合物荧光素丁酯（FL4）在溶液中和在二氧化钛（TiO_2）胶体中的吸收光谱、荧光光谱、荧光寿命等。

Based on the previous literatures, the following major innovation works were carried out in this dissertation:(1) The derivatization reaction conditions of ephedrine and pseudoephedrine with sensitive derivation reagent in aqueous system were studied and two new methods were developed for the assay of them by microemulsion electrokinetic chromatography and micellar electrokinetic chromatography with LIF detection. The sensitivity and analysis times were greatly improved compared with the previous reports;(2) A new method of CZE with indirect LIF detection was developed for the simultaneous determination of six coumarin compounds (esculin, esculetin, isofraxidin, genistein, naringin and sophoricoside) with fluorescein as the probe. The proposed method enlarges the application range of LIF detector, and provides new approach for the analysis of certain compounds difficult to derivatize;(3) Based on the above research, an MEKC with indirect LIF detection method for the simultaneous determination of adenine and guanine in DNA extracts from fungus, maize and soybean was established;(4) A new method for the investigation of the complexes formed between human serum albumin and ampicillin sodium under the simulated physiology conditions using laser light scattering technique was developed.

该论文在综述前人工作的基础上，开展了如下未见文献报道的创新性的研究工作：(1)使用灵敏的衍生试剂对麻黄碱和伪麻黄碱在水体系中的衍生反应条件进行了系统研究，建立了微乳电动色谱-激光诱导荧光检测法和胶束电动色谱—激光诱导荧光检测法测定麻黄碱和伪麻黄碱的灵敏分析新方法，与以前的报道相比，灵敏度和分析时间均有很大改善；(2)使用荧光素钠作为背景荧光试剂，建立了同时分析测定六种黄酮类化合物(秦皮甲素、秦皮乙素、异秦皮定、染料木素、柚皮苷和槐角苷)的毛细管区带电泳—间接激光诱导荧光检测新方法，扩大了激光诱导荧光检测的应用范围，对难衍生化合物的分离分析提供了一种新思路和新途径；(3)以荧光素钠作为背景荧光试剂，建立了一种用于同时测定食品提取DNA中的腺嘌呤和鸟嘌呤含量的胶束电动色谱—间接激光诱导荧光检测新方法；(4)用动态和静态激光散射法研究了模拟生理条件下人血清白蛋白与氨比西林钠盐相互作用所形成的复合物，为药物分子与蛋白质的相互作用、药代动力学等的研究提供了一种新思路。

Now the known species of luciferin was variety,which had large differences on structure?property and synthesis.At present the research is focus on the biosynthesis of luciferin.

现在已知的荧光素种类繁多，不同来源的荧光素在结构、性质与合成上大不相同，荧光素在生物体内的生成问题是目前研究的焦点。

Expression product had a 70kDa band and emitted yellow-green luminescence in the presence of luciferin.

云南窗萤荧光素酶在大肠杆菌中表达的条带大约70kDa，并且在有荧光素存在时发出黄绿色荧光。

Firefly can give rise to luminescence during the night, the basic reaction involved the firefly luciferase and luciferin and ATP in the body of firefly.

萤火虫在夜晚可以发出荧光，其基本的生物化学反应是虫荧光素酶可以催化荧光素生成氧化荧光素并且放出荧光，可以用以下这个反应方程表示：荧光素+ATP+O_2？

Firstly, the pGL3-Control vector was reconstructed , the pGL3-Enhancer vector deletious of SV40 promoter gene was obtained . Then the IFN-β promoter gene was cloned into the pGL3-Enhancer vector and pGL-IP21, the Luciferase reporter plasmid with IFN-β promoter was OK. The availability of pGL-IP21 was verified by NDV ,the inductor of IFN-β, the Luciferase activity was assayed in cells transfected with pGL-IP21 by Luminometer.

首先将pGL3-Control载体进行了改构，除去了SV40启动子基因，获得了pGL3-Enhancer载体，将获得的IFN-β启动子基因连入载体中，构建了带有人IFN-β启动子基因的荧光素酶报告质粒IP-21，并且通过实验证明所构建的质粒在干扰素的诱导剂NDV的作用下能够表达荧光素酶活性，照度计检测荧光素酶活性增强，从而验证了所构建的重组质粒的有效性。

These observations suggest that PVA-MEC is an excellent fluorescent macromolecular material with a convenient method of preparation and had a good water-soluble ability.(2) An acrylic monomer bearing xanthene group, acryloyl-fluorescein was synthesized from fluorescein and acryloyl chloride. The photophysical behaviors of Ac-Flu and its copolymer were explored by recording the fluorescence spectra in solution, solid state and film in detail.

2我们采用基于荧光功能单体制备荧光聚合物的方法，选择荧光素为发色团，使它与丙烯酰氯反应，合成出含有双键的荧光素单体Ac-Flu，然后令其与丙烯酰胺共聚，制备得到含有荧光素生色团的水溶性荧光聚合物poly(Ac-Flu-co-AM)。

Methods: A model to evaluate lymphocyte proliferation stimulated with a polyclonal activator, concanavalin A, was established by vital dye carboxyl fluorescin diacetate succinmidyl ester labeling technique. Effects of the different doses of anisomycin on the lymphocyte proliferation were estimated by flow cytometry and MTT methods. The propidium iodide labeling technique was applied to assay the effect of the different doses of anisomycin on changes of the lymphocyte cell-cycle stimulated by ConA or by phorbol ester plus Ionomycin. The percentage of the expression level of CD69 and CD25 on the activated lymphocytes was evaluated by fluorescin-conjugated monoclonal antibody double labeling technique.

以活体染料羧基荧光素乙酰乙酸琥珀酰亚胺酯染色，建立在多克隆刺激剂刀豆蛋白A刺激下评价小鼠淋巴细胞增殖的模型，通过流式细胞术和MTT法分析茴香霉素在不同剂量下对淋巴细胞增殖的作用；采用碘化丙锭染色分析茴香霉素对ConA或佛波醇酯加离子霉素刺激的小鼠淋巴细胞周期变化的作用；利用荧光标记的单克隆抗体双染技术和流式细胞术观察茴香霉素对小鼠CD3~+T细胞早期及中期活化标志分子CD69和CD25表达的影响。

In order to get purified purified commercially luciferase, we selected whole gene fragment of Photinus pyralis as well as gene fragment deleting 18-nucleotide at 5'termination, which was synthesized according to P〓 template.

为了能得到商品纯的虫荧光素酶，将取自P〓质粒中的全长的北美萤火虫荧光素酶基因片断，及以P〓为模板合成的5＇端缺失18个核苷酸的虫荧光素酶基因片断。

Advantages of specific coupling between biotin and avidin or streptavidin allow firefly luciferase to be immobilized on solid support coated with avidin or streptavidin, so that more flexible and convenient methods of luminescence assay will be available in application.

利用生物素与配体亲和素或链霉亲和素的特异性耦合，可以将荧光素酶固定到亲和素或链霉亲和素包被的磁珠上，从而使荧光检测的应用更加灵活和方便。

The absorption and distribution of chromium were studied in ryeusing nutrient culture technique and pot experiment.

采用不同浓度K2CrO4(0，0.4，0.8和1.2 mmol/L)的Hoagland营养液处理黑麦幼苗，测定铬在黑麦体内的亚细胞分布、铬化学形态及不同部位的积累。

By analyzing theory foundation of mathematical morphology in the digital image processing, researching morphology arithmetic of the binary Image, discussing two basic forms for the least structure element: dilation and erosion.

通过分析数学形态学在图像中的理论基础，研究二值图像的形态分析算法，探讨最小结构元素的两种基本形态：膨胀和腐蚀；分析了数学形态学复杂算法的基本原理，把数学形态学的部分并行处理理念引入到家实际应用中。