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A fusion gene of green fluorescent proteinand movement protein of cucumber mosaic viruswas obtained by using recombination-PCR.

采用重组-PCR方法,扩增获得绿色荧光蛋白与黄瓜花叶病毒运动蛋白融合基因,将其克隆到pKEN上,构建了该融合基因的原核表达载体pKENG-M。

The interaction between cucurbit(7)uril (Q[7]) and 5-aminosalicylic acid (5-ASA) at different pH was studied by fluorescence spectroscopy and 1 H NMR technique.

利用荧光光谱法考察了七元瓜环(Q[7])和5-氨基水杨酸(5-ASA)在不同pH条件下的相互作用。

The structures and fluorescence characteristics of cucurbit[ n = 6-8]urils with quinoline, isoquinoline and 7-methyl quinoline have been investigated by fluorescence spectrophotometry.

利用荧光光谱分析方法考察六、七及八元瓜环与喹啉、异喹啉、7-甲基喹啉相互作用的结构特征以及光学特性。

All of its characteristics, including the Shape, Colour, Clarity and Carat Weight are posted on it, along with specifications such as Polish, Symmetry, Girdle, Culet Size, and Fluorescence.

所有的特征,包括形状、颜色、清晰度和克拉重量都贴在上面,随着波兰等规格,对称束腰,culet大小、荧光。

There is a constant enhancement of fluorescence quantum yield when the cyanine dye complexes with HSA or BSA.

与人血清蛋白或牛血清蛋白结合后,噻菁染料的荧光量子产率增加。

The interactions of a cyanine dye with human serum albumin and bovine serum albumin have been investigated by using absorption and fluorescence spectra.

本文通过吸收和荧光光谱法研究了一种噻菁染料与人血清蛋白及牛血清蛋白的相互作用。

Further more, nonsulfonated cyanine dyes used for fluorescence labeling were designed andsynthesized.

另外,设计并合成了用于荧光标记的非磺化菁染料。

Table 2 The activity of Na+,K+-ATPase and cytochrome oxidase,fluorescent polarizationafter subacute exposure to acetone cyanohydrin ±s,n=6

表2 丙酮氰醇亚急性染毒大鼠红细胞膜和脑线粒体膜的Na+,K+-ATP酶、细胞色素氧化酶活力及荧光偏振度

The main equipment here is a thermal cycler system for nucleic acid amplification (PCR, quantitative fluorescence detection or common).

主要仪器就是核酸扩增热循环仪(PCR仪,实时荧光或普通的)。

Methods: Firty eight patients with prostate cancer and 112 healthy cases were selected as the control randomly. NAT2 and CYP1A1 gene polymorphisms were analysed with the methods of PCR RFLP, ASA and real time fluorescence Light Cycler.

应用PCR RFLP、ASA和自动实时荧光定量分析技术,分析 5 8例前列腺癌病人和 112例健康对照者CYP1A1和NAT2基因 4个位点的多态性,比较前列腺癌病人与对照组间频率差异。

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