荧光的

The curve of absorption is mirror symmetry of that of emission.

对荧光染料染色样进行了一系列的实验和测定。

Objective To study the diagnostic values of staphylococcal protein A-enzyme linked immunoadsorbent assay and mite antigen piece-indirect fluorescent antibody test on pulmonary acariasis.

目的 探讨葡萄球菌A蛋白-酶联免疫吸附试验和螨成虫抗原片间接荧光抗体试验对肺螨病的诊断价值。

A novel analytical method has been established for on-line simultaneous determination of fluorene and acenaphthene by polarization synchronous fluorometryhyphenated with HPLC.

本文以高效液相色谱作为分离手段，以偏振同步荧光法为检测手段，实现了环境样品中芴、苊的在线同时测定。

Objective To investigate the heterogeneity of chemosensitivity in gastric cancer using adenosine triphosphate based tumour chemosensitivity assay and the feasibility of individual chemotherapy.

目的采用三磷酸腺苷生物荧光法对胃癌组织标本进行肿瘤药敏检测，为胃癌的个体化化疗选择提供依据。

After the cell growth curves was recorded, RPE cells of the 3-5th passages were utilized. 2、Three different siRNA (siRNAl,siRNA2,siRNA3) targeting against human cx43 gene and one negative control siRNA were designed and transfected into cultured human RPE cells via liposome reagent. The most effective siRNA can be determined by semi-quantitative reverse transcription PCRRT-PCR. 3、To the most effective siRNA, after transfected into human RPEs with different concentration, the cellular proliferate activities were messured by MTT colorimetry ; the percentages of RPE in different cell circle phase was assayed by FCM; the changes of phenotypical properities were observed with SCM; the protein expression of cx43 was studied through immunocytochemistry stain and Weston blot; the communication intercellular was calculated with FRAP; and the ability of recovery was assessed by using an in vitro wound healing model.4、The total proteins of siRNA1 and RPE were seperated by two-dimensional gel electrophoresis and visualized by silver staining. Proteins with significant expression alterations were selected and their peptide mass fingerprints (PMFs were obtained by matrix-assisted laser desorption/ionization time of flying mass spectrometry (MALDI-TOF-MS).The PMFs were used to search NCBInr database by Auto MS-Fit software.

实验方法：1、培养原代的人RPE细胞，经过细胞角蛋白、S-100和神经胶质原纤维酸性蛋白免疫细胞化学鉴定后，通过AO/PI染色技术确定培养细胞的存活率，描记其生长曲线，第3-5代用于以下细胞实验2、生物合成针对人cx43基因的三条小干扰RNA和一条阴性RNA通过脂质体转染RPE细胞后，通过RT-PCR的方法确定抑制效率最高的干扰片断3、将该片段以不同浓度通过阳离子脂质体转染培养的人RPE细胞后，采用MTT法观察其对细胞的增殖力的作用；通过流式细胞仪观察其对细胞周期的影响；通过扫描电镜观察其对细胞形态的影响；通过免疫细胞化学和Weston blot观察其对cx43蛋白表达的作用；采用激光共聚焦和荧光淬灭恢复技术观察荧光恢复速率平均百分率，评价其对细胞间通讯功能的影响；通过制作RPE细胞损伤模型，观察其对损伤修复能力的作用4、分离纯化转染siRNA的RPE组和正常对照组RPE细胞的全部蛋白质，应用等电聚焦电泳和SDS-PAGE双向电泳技术，银染显示分离出的蛋白质斑点，经凝胶图像分析软件对两个样本进行胶图分析，寻找差异蛋白点。

Most of water-soluble cyanine dyes have -SO3H and –COOH in their molecules, which will influence the biomolecules' functional characters. A new kind of water-soluble cyanine dyes with several cations in molecules were designed and synthesized, preparation conditions of intermediate and dyes were researched and optimized, the yield of dye is 25%. The novel kind of cyanine dye are water-soluble , spectrum characters was researched , all the dyes were charactered by 1H NMR and TOF-MS, spectrum in different solvent of novel dyes were focused on, one of the kind dyes show higher fluorescence quantum yield than the result reported. Because of several cations in the structure, it is promising when used in DNA assay and other bio-assay field.

大多数水溶性的菁类荧光染料分子结构中都带有-SO3H和-COOH等基团，过多的阴离子的引入容易造成对生物分子的功能属性的影响，本论文设计并合成了分子结构中带有多个正电荷的水溶性的一类新型菁染料，考察并且研究了中间体和染料的合成条件，最终的目标产物产率达到25％，染料通过了质谱和核磁的表征，新型的染料具有良好的水溶性，研究了新染料在多种溶剂的的光谱性质，其中一种染料的在水中的荧光量子产率比文献报道得到了提高，由于富集了正电荷，这类菁染料在DNA标记等生物中有一定应用前景。

A flat high efficiency super-broadband source was presented. We achieved a C+L-band superfluorescent source, in the region from 1524. 0 to 1600.4nm, the power of the ASE are over -12. 0dBm and the ASE profile excursion is only ±1. 4dB in the region from 1539. 2 to 1600.4nm. The power of the ASE is 22. 1mW and the power conversion efficiency is 18. 8%. 9. The amplification characteristics of thulium-doped fiber amplifier had been studied using the basic theory for fiber amplifier. The connection between the Tm-doped fluoride fiber and silicon fiber was accomplished, and we studied the TDFA and the reason that the expecting results hadn't achieved had been analyzed. The assumption of the Er-Tm-co-doped silicon fiber had been presented, and we studied the Er-Tm-co-doped silicon in cooperation with the correlative corporation, some results had been got and the further studies are carried on.

实验结果得到了覆盖C+L-波段的超荧光光源，在1524.0-1600.4nm波长范围内，自发辐射谱的功率均高于-12.0dBm；在1539.2-1600.4nm波长范围内，自发辐射谱不平坦度为±1.4dB；荧光总功率为22.1mW，功率转换效率为18.8%； 9、应用光纤放大器的基本理论，理论分析和研究了掺铥光纤放大器的放大特性；解决了氟化物掺铥光纤与硅基光纤的连接问题，进行了TDFA的实验研究，分析了没有得到预期结果的原因；最后提出了铒-铥共掺硅基光纤的构想，并与有关单位合作，共同研制了铒-铥共掺硅基光纤，得到了一些前期结果，进一步的研究仍在进行当中。

Fluorescence emission spectra and UV absorb spectra analysis have been undertaken to test the influence of solvent polarity on the lowest excited singlet state of the tertiary amines. The absorption maxima were red-shafted slightly, and the emission maxima were red-shafted strongly, which showed all of the three compounds were found to be essentiallyππ* in character, which makes for the formation of triplet-exciplex state and the subsequent reactions,?known as photo-reduction and hydrogen-abstraction.

本文借助光谱检测的手段测试了新合成的增感剂的光物理化学性能，利用荧光发射光谱和紫外吸收光谱测试了溶剂极性对叔胺的最低激发单重态的影响，实验结果表明叔胺的紫外最大吸收波长随溶剂极性的增强产生轻微的红移，荧光最大发射波长随溶剂极性的增强产生明显的红移，表明三种叔胺的最低激发单重态具有ππ*跃迁的特性，这对于激态复合物的生成以及其后的光致还原反应和提氢反应的发生都非常有利。

By suspending cell culture from the callus of P. thugbergii and by fluorescent observation, a new method, which is easy and sensitive to observe cell activity and to test the phytotoxicity of various bacteria, was used. This method can be utilized to study the bacterial toxins. Cultured filtrates of some strains of the nematode-carrying bacteria were bioassayed for their toxicity to suspending cultured cells from P. thugbergii callus. Strong toxic effects were observed by the filtrates of Pseudomonas spp., P. fluorescence, and Sphingomonas spp., these bacteria species are coincide with those in the early stage of pine wilt disease.

本试验使用了黑松愈伤组织的细胞悬浮培养方法，用荧光方法观察各种细菌对植物细胞的毒性测定，这种方法具对细胞的活性观察容易、灵敏度高等特点，证明可用于细菌的毒素研究；对松材线虫携带的细菌的部分菌株，用其培养滤液对黑松愈伤组织的悬浮培养细胞的生物测定初步证明，松材线虫携带的细菌中以假单胞菌、荧光假单胞菌和少动鞘氨醇单胞菌的滤液对黑松细胞具有较强的毒性，这与松树发病早期出现的细菌种类相一致。

Therefore, the EL spectra of (Znq_2)_4 is wider than that of Znq_2. 4._2 was synthesized. The analysis of molecular spatial structure and the characterization of material performance of_2 and Liq showed that two Liq molecules and two Naq molecules were connected by Na-O-Na bond bridges to form_2. Compared with Liq,_2 exhibits stronger rigidity in planar molecular structure, larger steric hindrance and intermolecular distance, and much smaller molecular polarity, thus resulting in much longer fluorescence lifetime, much higher fluorescence quantum efficiency, wider energy bandgap and better film formability. When used as light-emitting layer in OLED,_2 shows lower formation probability of excited dimmer and exciplex formation than Liq, thus emits bluer light with higher current efficiency than Liq. When_2 ultrathin film is used as electron injection layer in OLED, it exhibits higher current density, higher luminance, lower turn-on voltage and higher current efficiency than Liq ultrathin film for the existence of sodium ions in_2 ultrathin film. 5. The summarization the relationship between molecular spatial structure and material performance of Alq_3,(Znq_2) and Liq, lead the conclusion that the molecular spatial structure of Mq_n affects its material performance in such aspects as the rigidity of planar molecular structure, intermolecular interaction, molecule stacking mode and intermolecular distance.

制备了_2，通过对_2和Liq的分子空间结构与性能进行分析与讨论，发现_2是通过两个Na-O-Na键桥将两个Liq和两个Naq连接构成的，其分子平面结构的刚性程度强于Liq，空间位阻大于Liq，分子之间的距离大于Liq，分子极性远远小于Liq；_2的荧光寿命长于Liq，荧光量子效率高于Liq，成膜性优于Liq；_2的禁带宽度比Liq大，光致发光光谱中_2的最大发射峰较Liq发生蓝移；当_2在OLED中作为发光层时，激发二聚体与激基复合物的生成几率远远小于Liq，发的光比Liq更蓝，电流效率大于Liq；_2超薄膜中有Na离子的存在，与Liq超薄膜相比，当其在OLED中作为电子注入层时，具有更大的电流密度，更高的发光强度，更低的阈值电压和更高的电流效率 5、对上述Alq_3、Znq_2和Liq的分子空间结构与材料性能之间的关系进行了归纳总结，认为Mq_n的分子空间结构主要在分子平面结构的刚性程度，相邻分子之间的相互作用，分子堆叠的方式和分子之间的距离这四个方面影响其性能。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。