荧光的

In part one, we prepared three ramification of pyrene nanoparticles by reprecipitation method under ultrasonic radiationand characterized with TEM.The determination of organic nanoparticles to DNA, NO2 -,and nucleic acids werestudied by using fluorescence. And some ultrasensitive determination of DNA, NO_2~-, and nucleic acids methods were proposed. The possible mechanism has been studied.

第一部分在超声辐射下用再沉淀的方法制备了几种芘的衍生物的纳米粒子荧光探针，并通过透射电镜进行了表征，并通过荧光法研究了它们分别与蛋白质、核酸以及NO_2~–的作用，建立了超灵敏的检测上述物质的方法，探讨了作用机理，并提出了合理的解释。

Results showed that the new type rhodanine ramifications were good performance fluorescence reagents to determine the trace matter by fluorescence analysis.

同时，通过对上述试剂在测定铁、钴、铜、锡和锑时的条件的变化的研究，对这类试剂的分子结构和荧光性能的内在关系作了简要的探讨，讨论了供、吸电子基团对胂酸基偶氮若丹宁类衍生物荧光分析性能的影响。

A new emission peak located at long wavelength (at about 515 nm) appeared in PL spectra of the unsubstituted polymer (PF6P) films annealed in air, and the intensity of the long wavelength emission increased with the increase of annealing temperature or annealing time.

苯环上未取代聚合物PF6P的薄膜在空气中退火后在长波(515 nm)出现一个新的荧光发射峰，随退火温度的升高或退火时间的延长，该长波荧光发射峰的强度逐渐增大，薄膜的紫外吸收峰变宽。

The relationship of the amounts of emulsifier, organosilicon monomer (D4) and initiator with the ratio of fluorescence intensity between first vibronic peak of pyrene (λ= 373 nm) and third vibronic peak of pyrene (λ=384nm)Ⅰ1/Ⅰ3 were discussed during the emulsion polymerization process, in which pyrene was used as fluorescence probe. The polymerization behavior of the organosilicon-acrylate latex with core-shell structure was investigated in line with the relationship of monomer conversion and development of morphology in latex particles with the value of Ⅰ1/Ⅰ3 for probe pyrene during the polymerization process.

以芘为荧光探针，探讨了有机硅-丙烯酸酯核壳乳液聚合过程中，芘的第一振动峰(373nm处)与第三振动峰(384nm处)荧光强度的比值Ⅰ1/Ⅰ3与乳化剂、有机硅单体（D4）和引发剂用量之间的关系，并结合聚合过程中探针芘的Ⅰ1/Ⅰ3 峰值与单体转化率及乳胶粒形态演变之间的关系，研究了核壳结构有机硅-丙烯酸酯乳液的聚合行为。

The results shown that both [Cu_2]~2+ and NR molecular can intercalates into the DNA double helix layer, and there is a competition between these two interactions. Furthermore, chemometrics approach, parallel factor analysis, was used to resolve the measured three-way CW-SFS data, which provided simultaneously the concentration information for the three reaction components, NR, [Cu_2]~2+ and NR-DNA, for the system at each equilibrium point. This PARAFAC analysis indicated that the intercalation of the [Cu_2]~2+ molecule into the DNA proceeds by exchanging with the NR probe, and can be attributed to two parallel reactions.

本工作以同步荧光技术采集了竞争作用的三维数据，并结合了化学计量学中的平行因子法对此三维数据进行分解，得到了各组分的荧光光谱图及各个反应平衡点各组分的相对浓度，平衡浓度的变化趋势也表明了铜络合物与中性红之间的竞争作用，进一步表明了铜络物与DNA的作用方式和中性红与DNA作用方式一样均为嵌插作用。

In order to examine the influence of higher ionic strength on the interaction between QDs and avidin, the fluorescence spectra of free CdTe and CdTe-avidin solution are recorded as a function of increasing NaCl concentration.

实验还考察了盐浓度对QDs-亲和素体系的影响情况，结果发现随着盐浓度的增大，QDs-亲和素体系的荧光峰位逐渐蓝移，而单独量子点的荧光峰位基本不变，这说明盐浓度的增加削弱了CdTe量子点与亲和素分子之间的结合作用。

Shape of fluorescence spectrum of diphenylanthracene in multilayer film is almost the same as that in solution, and the position of emission peak does not show apparent shift, which is related to the fact that fluorophore are surrounded by highly branched dendrons. These results provide a new route for design and assembly of luminescent materials.

溶液中的荧光光谱和多层膜的荧光光谱的形状和峰位都非常接近，这和发色团处在树状分子的三维支化结构的中心是直接相关的，这一研究对发光材料的分子设计具有重要的指导意义。

When energy transfer systems were obtained from the interaction of G1.0, G1.0, G and G2.0 with eosin or Eu~(3+) ion respectively, all the dendrons could partially transfer their absorbed energy to eosin and Eu~(3+) ion at a certain range of concentrations.

将G1.0、G1.0、G(来源：c2ABC论文网www.abclunwen.com)、G2.0分别与曙红或Eu~(3+)离子构成能量转移体系，发现在一定的浓度范围内，这些树枝块均可以将吸收的能量转移给曙红和Eu~(3+)离子，使曙红和Eu~(3+)离子的特征发射强度增强，曙红的荧光强度最大增强为原来的2.5倍；Eu~(3+)离子的荧光强度最大增强为原来的11.7倍。

This article summarises the chemical reactions in milk during heat treatment, analyzes the properties of several indicators for heat damage , such as heat-sensitive enzymes, denatured proteins, the products of Mailliard Reaction and isomerised lactose.

本文分析了牛乳在热加工过程中的化学变化，介绍了几种牛乳热损伤监控标识物(如热敏性酶类，蛋白质变性产物，美拉德反应产物和乳糖异构化产物)的特点，并详细论述了已有的牛乳热损伤监控技术：对变性β-乳球蛋白含量的监控，对糠氨酸和乳果糖含量综合水平的监控，对羟甲基糠醛含量的监控，以及以变性蛋白质(以色氨酸的荧光强度计，FTry)和美拉德反应荧光产物的聚集量为基础，评估牛乳热加工损伤程度的FAST方法。

Oocyte samples from one group were collected to detect the presence and integration of HBV DNA within cells and chromosomes using PCR, Southern blot, dot hybridization and fluorescence in situ hybridization. The female animals from another group were mated with their normal males, respectively. Their zygotes, 2-cell embryos were collected to detect the integration of HBV DNA in the female pronuclei of zygotes and the replication and expression of HBV genes in the 2-cell embryos using FISH, RT-PCR and immunofluoresence assay.(1) PCR detected positive bands in the tested oocyte samples fromgoldon hamster and mice. Southern blot revealed clear hybridization signals in PCR products.

研究用金黄地鼠和小鼠建立实验动物模型：将卵巢内注射HBV DNA的实验动物分成两组，一组注射后进行超排卵，收集卵巢和输卵管的卵母细胞，用PCR、Southern杂交，斑点杂交和荧光原位杂交(fluorescence in situ hybridization、FISH)检测HBV在卵母细胞内的存在和染色体上的整合；另一组超排雌鼠与正常雄鼠合笼，收集受精卵和2-细胞胚，用FISH、RT-PCR和免疫荧光检测技术分别研究HBV基因在受精卵雌原核上的整合以及在2-细胞胚中的复制与表达。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。