荧光的

To understand the mechanism of the luminescent enhancement for the nanocomposites,the measurements of steady fluorescence emission,fluorescence lifetime and spin-lattice relaxation time ( t 1) of the individual carbon atoms in MEH-PPV for the nanocomposite films were carried out.

结果激发态激子自淬灭的几率降低，稳态荧光量子产率提高和动态荧光寿命的延长；通过共轭碳原子的自旋-晶格弛豫时间的测定，观察了MMT对聚合物分子运动的影响，并由此探讨了MEH-PPV凝聚态与光物理性质的关系。

Methods］ MTT assay was employed to test the lethal concentration 50 (IC50) of sensitive germline (SK-OV-3) and ovarian cancer cell subline induced by ADR (SK-OV-3/adr) treated by 4 drugs, resistance index and reverse multiple were calculated. The changes of drug content were observed by fluorescence microscope, the intracellular accumulation of ADR was evaluated by fluorospectrophotometry, the changes of mdr 1/P-gp resistance were observed respectively by RT-PCR and Western blot.

方法］MTT法检测药物对敏感细胞株（SK-OV-3）和诱导的耐药细胞株（SK-OV-3/adr）的半数致死浓度（IC50），计算耐药指数和加逆转剂后的逆转倍数，荧光显微镜观察不同时间细胞内的药物含量变化，荧光分光光度法测定细胞内ADR的含量，RT-PCR和Western blot法检测细胞耐药前后mdr1/P-gp的变化。

Fan YS,Davis LM,Shows TB.Mapping small DNA sequences by fluorescence in situ hybridization directly on banded metaphase chromosome.Proc Nat1 Acad Sci USA,1990,87∶6223-6227.5 Cherif D,Julier C,Delattre O,et al.Simultaneous localization of cosmids and chromosome R-banding by fluorescence microscopy:Application to regional mapping of human chromosome 11. Proc Nat1 Acad Sci USA,1990,87∶6639-6643.6 Harai M,Suto Y,Kanoh M.

我们曾经介绍过一种荧光R显带的方法［10］，在此基础上建立了一种在显带染色体上直接观察杂交信号的新的定位方法：在细胞培养时，同时加入BUdR和Hoechst33258，PI染色后即呈现清晰的荧光R带带型，用彩色和全色黑白胶卷照相均可得到满意的结果，尤其适用于国内具有一般条件的实验室。

Methods Calcein-AM stained H9/HIV-1 ⅢB cells were treated with difierent concentrations of PF in the first,second and third trimesters respectively,which was then mixed with MT2 cells and put under fluorescent microscopy for detection of HIV-1-mediated syncytium formation.MT2 cells were treated with cell-free HIV-1 Ⅲ B,and then washed and cultured with different concentrations of PF in the first,second and third trimesters respectively.Furthcrly,the protecting effect of PFs against HIV-1-infected cells Was determined with MTT,viral replication Was evaluated by measurement of the levels of p24 antigen in culture supernatants with ELISA and the inhibition rate Was calculated.

采用荧光染料Calcien-AM标记的H9/HIV-1 ⅢB分别与早、中、晚孕期不同稀释浓度的PF作用后，与MT2细胞混合培养，荧光显微镜下观察合胞体的形成；用游离的HIV-1 ⅢB感染MT2细胞，并分别与早、中、晚孕期不同稀释浓度的PF作用后，用MTT法检测HW-1感染细胞的存活率，用HIV-1 p24抗原试剂盒检测细胞培养上清中p24抗原含量的变化。

HRP was coupled with the fluorochrome FITC and encapsulated with liposome . pEGFP-N1 plasmid (expressing green fluorescent protein) with encapsulation in commercially available liposome was prepared. The eyes were enucleated 1, 4 and 8 weeks after zonule rupture and anterior segments comprising lenses were incubated in medium containing one of these components. Cryo-sections were made and translocation of fluorescent macromolecule from the medium into the lens and green fluorescent protein expression in the epithelium were observed by fluorescent microscopy.

制备FITC标记的HRP脂质体；制备pEGFP-N1质粒（表达绿色荧光蛋白的质粒），并用脂质体包裹；豚鼠悬韧带部分离断后1周、4周、8周取含晶状体的眼前段标本分别在含有FITC-HRP复合物、pEGFP-N1质粒的培养基中孵育，冰冻切片，荧光显微镜观察FITC-HRP复合物进入晶状体和pEGFP-N1质粒在晶状体内表达的情况，比较悬韧带离断侧与非离断侧的差异。3。

The results have indicated that the introduction of electron-donating methoxy or electron-withdrawing cyano-group at the 7-position of 3-phenylcoumarins caused a bathochromic shift for both absorption and fluorescence spectra. The phenyl group at the 4-position derivative has a hypsochromic shift for both absorption and fluorescence spectra.

结果发现，在3-苯基香豆素的7-位引入给电子基团甲氧基或吸电子基团氰基均使它们的吸收光谱和荧光光谱产生红移，4-苯基香豆素的衍生物的吸收光谱和荧光光谱均产生蓝移。3-苯基香豆素衍生物与4-苯基香豆素的衍生物的基态和激发态的电子转移方向相反。

Methods The primer design is referring to thearticleof Bulter. Over 120 samples provided by the laboratoryare detected to verify the concordant results between miniSTR andcommon STR. We construct the miniSTR multiplex system usingdomestic Taq DNA polymerase, and detect the results by ABI—310Genetic Analyzer. We have alsostudied the sensitivity, accuracy, species-specificity and different PCR outcome under each annealtemperature.

方法参照Bulter等对THO1、TPOX、CSF1PO三个基因座miniSTR的引物设计，选用本教研室提供的123例无血缘关系个体的血样，进行THO1、TPOX、CSF1PO三个基因座PCR-miniSTR银染检测，检测结果与商品化试剂盒的检测结果进行一致性检验；采用国产DNA聚合酶构建miniSTR荧光标记复合扩增体系，用美国ABI-310基因分析仪进行检测；对该荧光标记复合扩增体系的灵敏度、准确性、种属特异性、不同退火温度下复合扩增的效果、陈旧血痕DNA的检测等进行研究。

The fluorescence intensity of these chitosan derivatives increases and decreases with the different chromophoric group in the polymers\' long-chain. The fluorescence properties of these chitosan derivatives are also changed with the different pH, solvent and concentration

首次考察了分子量为104数量级的壳聚糖衍生物的荧光性能与其侧链上的生色团、助色团的关系，以及在不同浓度、不同酸度、不同溶剂中它们的荧光性能的变化情况来源：33ddABC论文网www.abclunwen.com

Excitation-Emission Matrix Fluorescence Spectroscopy was employed to study fluorescence characteristics of chromophoric dissolved organic matter readily released from sediment particles during episodes of resuspension, and bottom waters and surface sediment pore waters were compared in all corresponding sites.

对采自厦门湾九龙江入海河口的4个沉积物样品进行了室内再悬浮模拟实验，利用荧光激发－发射矩阵光谱研究了再悬浮过程中从沉积物中释放出的有色溶解有机物的荧光特征，同时通过与相应站位沉积物间隙水和底层水的对比分析，探讨了河口近岸海域的沉积物再悬浮作用作为水体中溶解有机物来源之一的可能性。

CuRF coupounds are based on the combination of deprotonation and ICT mechanisms. The secondary amines conjugated to 1,8-naphthalimide could be deprotonated by Cu2+, and as a result, large red shifts in both absorption and fluorescence spectra were obtained, from which one could sense Cu2+ colorimetrically and ratiometrically.

CuRF 系列则是基于脱氢和ICT原理的结合，铜离子与受体的作用能够诱使共轭氮原子上的氢脱去，共轭氮原子显负电增强了对荧光团的供电能力，从而引起荧光波长的红移，同时也造成了吸收光谱的红移。

But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊！不用提了。提到肉，真是糟透了。