荧光的

Based on the study of spectrum, the components and technology requirement of time-resolved fluorometer were presented And a concrete method to actualize the system of pulsed Xenon lamp and the assemblage of filter setting was developed Sound clinical evaluation project was designed At last, clinical evaluation of the time-resolved fluorometer was conducted.

在光谱研究的基础上，讨论了时间分辨荧光免疫分析测试仪的各个组成部分及其技术要求，提出了脉冲氙灯和聚光滤光系统的具体实现方法，制定了合理的临床评价方案，并对制成的测试仪样机进行了临床应用性能实验。将我们研制的时间分辨荧光免疫分析仪与国外先进同类型仪器进行一系列对比测试实验后，经相关回归分析所得出的相关系数都在99.5％以上，显著性检验小于0.01。

The silicon germanium oxide composite nano line produced in the method has the properties of good fluorescence activity, large specific surface area, adjustable length and thickness; thus the silicon germanium oxide composite nano line is expected to be applied to a plurality of fields such as advanced catalyst design, biological fluorescence label, nano component assembly, microscopic conduction and synthesis of advanced optical, electrical and magnetic material and has potential application value.

通过该方法制备的硅锗氧化物复合纳米线具有很好的荧光活性、大的比表面积以及粗细、长短可调控的性质，因此这种硅锗氧化物复合纳米线有望在许多领域例如先进催化剂的设计、生物荧光标记、纳米器件组装、微观传导以及先进光学、电学和磁学材料的合成中有潜在应用价值。

Forty-eight hours after transfection, cells were harvested to determine luciferase activity by illuminometer, In the presence of RU486 a 40-fold maximum activation of the luciferase reporter gene was observed in cultured cells, whereas in the absence of RU486, no significant activation was observed.

加入诱导剂RU486后，可以诱导表达荧光素酶，并在一定范围内两者呈正比，最高可以实现荧光素酶的40余倍的表达，而没有RU486时，几乎没有报告基因的表达，表明RU486诱导调控载体构建成功，可实现对目的基因的表达时间和表达水平的精确调控，为进一步的基因调控研究和基因治疗提供了良好的工具。

Forty-eight hours after transfection, cells were harvested to determine luciferase activity by illuminometer. In the presence of RU486 a 40-fold maximum activation of the luciferase reporter gene was observed in cultured cells, whereas in the absence of RU486, no significant activation was observed. There was a positive correlation between the luciferase activation and RU486 concentration in a definite range. The results showed that the RU486-inducible regulatory vector was successfully constructed, which can be used to regulate the expression level of genes of interest in appropriate time by the inducer RU486. This inducible expression vector provides a platform for the research of gene regulation and gene therapy.

加入诱导剂RU486后，可以诱导表达荧光素酶，并在一定范围内两者呈正比，最高可以实现荧光素酶的40余倍的表达，而没有RU486时，几乎没有报告基因的表达，表明RU486诱导调控载体构建成功，可实现对目的基因的表达时间和表达水平的精确调控，为进一步的基因调控研究和和基因治疗提供了良好的工具。

NGF had obvious cytotoxicity on these cell lines ,which shows to be dose-dependent, especially for MGC-803 and BEL-7404 ,their IC_(50) value at the 24~ hours are 49.9mg/L and 55.46mg/L ; Main features of apoptosis under fluorescent microscope include reduction in volume,nuclear chromatin condensation, intensification under jacinth fluorescence; The early apoptosis rate of pristine cells which detected by FCM increases with NGF dose increasing.

2NGF对4种人肿瘤细胞均有细胞毒性作用，且呈剂量依赖关系，尤其是对MGC-803和BEL-7404的抑制作用更强，作用24h的IC50值分别为49.9mg/L和55.46mg/L，荧光显微镜下可观察到MGC-803凋亡细胞的主要特点为：细胞体积缩小，染色质固缩，橘红色荧光增强；流式细胞仪检测到MGC-803细胞的早期凋亡率随NGF浓度的增高而增加，各浓度组与对照组的早期细胞凋亡率比较有明显差异(P＜0.01)。

The recent progresses in the study on the fluorescence of rare earth complexes and application for the determination of the rare earth elements and organic compounds, as fluorescent probes for bioactive materials analysis and as detection means in separation technology were reviewed in detail. In chapter two, the absorption spectra of the 4f electron transitions of the complexes of the neodymium, praseodymium, holmium and erbium with fleroxacin in the presence of CPC have been studies by normal and derivative spectrophotometry.

本文以稀土元素配位化学为基础，以导数吸收光谱技术和荧光光谱技术为研究手段，利用新一代喹诺酮类抗菌药物，抗凝血类杀鼠药物的化学结构特点和大环化合物冠醚与金属离子突出的配位能力，以分析应用为目标，系统研究了这些化合物同稀土元素形成的配合物的光吸收和荧光特性，通过对体系实验条件的优化，使体系的稳定性、选择性和灵敏度得到显著改善。

The object of this study is to cancerous and normal cells intimacy of hematoporphyrin derivative for diagnosis and therapy cancer, fluorescence spectra of cancerous and normal cells are measured using a ultrashort pulses laser spectral technique and picosecond time-correlated single-photon counting system.

研究用于癌症诊断与治疗的光敏剂血卟啉(hematoporphyrin derivative， HPD)对人体癌细胞与正常细胞的亲和性，采用超短脉冲激光光谱技术和皮秒时间相关单光子计数系统，测量经血卟啉培养的活体癌细胞与正常细胞的荧光光谱，观测到癌细胞样品在创645nm处具有特有的光谱谱峰；测量不同浓度血卟啉荧光光谱，观测到随着血卟啉浓度的逐渐增大，在645nm处出现的光谱峰也随之显著增强。

Thus, in the electrophysiological aspect of CNS, the excitatory model was set up and the effect of JuA on the bioelectrical activity of the CNS was tested. The results were comparable with those obtained in the experiments of western medicine, eg. Phenobarbital sodium.

实验中比较了两种不同的Ca〓荧光探针Fluo-3和Calcium Orange的染色条件及荧光发射特点，分析了F1uo-3在检测Ca〓快速反应变化中的优点，而Calcium Orang比较能够体现Ca〓的空间分布差异，国内尚无有关类似的结果的研究报道。

It was based on the quenching of the fluorescence of RVN in the presence of DNA.

DNA的存在使RVN的荧光猝灭，而且荧光猝灭的程度与加入DNA的量在一定的浓度范围内呈现良好的线性关系。

A GFP-labeled Hela cell line and a mouse tumor model based on the cell line were established, and the animal model provides useful experimental materials for cervical cancer study. The in vivo fluorescence imaging system could be used to quantitatively evaluate the living tumor cell growth in vivo but not the size or volume of the tumor detected by slide caliper.

绿色荧光蛋白能够在人宫颈癌细胞Hela中长期稳定表达，用绿色荧光蛋白标记的人宫颈癌细胞Hela建立的裸鼠肿瘤模型可以为人宫颈癌研究提供理想的实验材料，应用小动物活体成像系统能够客观定量评价活的肿瘤细胞在动物体内的生长情况，而不是肿瘤体积的变化。

But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊！不用提了。提到肉，真是糟透了。