荧光的

Through simple Gaussian line fits, we found not only obvious Fe K line variability with no significant difference in the X-ray continuum flux between two ASCA observations which were separated by～440 days, but also rapid variabilities of Fe K line on time scales～10〓s within the second observation.

使用简单的高斯线拟合我们发现，在间隔440天左右的两次ASCA观测之间，NGC 4051的铁Kα荧光线轮廓和强度有着明显变化，而与此同时X射线连续谱流量保持不变；不仅如此，我们发现在第二次ASCA的观测过程中，NGC 4051的铁Kα荧光线有着10〓s尺度上的快速变化。

In this work, the interaction between umbelliferone and bovine serum albumin was studied by performing two different kinds of titration modes. The two spectroscopic data matrices were augmented, and then the information of experiment data was increased. The augmented experiment data matrix was analyzed by the MCR-ALS method. This procedure allowed the recovery of the concentration profiles for each species and the calculation of the ∶ ratio in the complex and the apparent equilibrium constant.

采用两种不同的试剂滴加模式对伞形花内酯与BSA的相互作用进行研究，对经典的荧光光谱数据矩阵加以扩展，增加了实验数据的信息量；进而应用MCR-ALS对该扩展的荧光光谱矩阵进行迭代计算，较好地分辨出作用过程中浓度变化趋势图，并计算出伞形花内酯与BSA的表观结合常数和结合比。

The fluorescence anisotropy using steady-state fluorescence polarization method was measured for the solution of β-CD and cinchonine mixed and sonicated for 1h. The anisotropy values for the solution increased with the increase of the concentrations of β-CD, which implied the existence of some complexes of large size or nanotube structure. Many type of large size complexes formed between β-CD and Cin under high concentrations in gas phase were characterized by ESI-MS in moderate experiment conditions.

将辛可宁与环糊精的混合溶液超声1个小时后，用稳态荧光偏振方法测定溶液的各向异性值，发现随着环糊精浓度的增大，溶液的荧光各向异性不断增强，这意味着溶液中形成了大尺寸即纳米级结构的环糊精与辛可宁复合物；用温和的质谱测定条件表征发现高浓度的β-CD与辛可宁在气相中可形成各种类型的大尺寸复合物。

At last the conductibility of base and doping conducting polymer was mensurated The researchful result shows that the new conducting polymer ,whether doping or not, all have better fluorescence and conductivity at the same time, and the complexes have better fluorescence coordinated with Al3+、Zn2+ in solution.

经上述的研究表明，新型的导电聚合物无论是本征态还是掺杂态，既具有比较好的导电性，同时具有比较好的荧光特性，在溶液中与Al3+、Zn2+配合形成的配合物具有较好的荧光特性。

Three RNAi target sites (named as Abil1, Abil2, Abil3) targeting the E3B1 (NCBI: NM-024397), an identified target sites and positive control GAPDH-A were selected. The pGenesil-1 eukaryotic expression vectors with the neoR mark and GFP green fluorescent mark were selected to construct E3B1 RNAi plasmid. The effect of RNAi targeting various sites on E3B1 genes expression was evaluated by testing the transfection efficiency with fluorescence microscope and western blot. The most effective siRNA in inhibiting E3B1 genes were screened. The most effective siRNA in inhibiting E3B1 genes screened and used to transfect the neuronal cells. After that, the inhibitor of axon growth was added and the axon growth was observed. Result: The siRNA expressing plasmids targeting the E3B1 were constructed successfully.

选择针对E3B1(NCBI： NM-024397)RNAi的3个靶位点Abil1、Abil2、Abil3和1个不针对任何mRNA的RNAi靶位点以及甘油醛－3-磷酸脱氢酶，用带有neoR选择标志和GFP绿色荧光标志的真核表达载体pGenesil-1构建E3B1 RNAi质粒，分别转染培养的神经元，在荧光显微镜下观测转染率，经G418筛选得到单一的转染细胞，并用Western blot法检测各转染组神经元E3B1蛋白的表达情况，选出具有最佳抑制效应的siRNA转染神经元，加入轴突生长抑制物，观测轴突生长情况。

Second,the development trend and research status of fluoroquinolones was presented. Fluoroquinolones,which were newly-developed oral comprehensive antibiotics in recent years,has fairly strong flurorescent quality and therefore is used to explore the functional mechanism of drugs in human bodies,by the change of fluorescent extensity between fluoroquinolones and protein,as well as metallic irons.

其次是氟喹诺酮类药物的发展趋势及研究进展，氟喹诺酮类的药物是近年来迅速发展的口服广普抗菌药，具有较强的荧光特性，因此可以利用其与蛋白质及金属离子相互作用时荧光强度的变化来研究药物在人体内的作用机制等。

The binding of bis-ANS bound to blue membrane at different temperature are only one binding sites. 4. The research results of fluorescence, absorption spectra and stopped flow method used to track the process of transition from acidifying purple membrane to blue membrane indicate that the transition of the characteristic absorption band of retinal(from 570 to 605 nm) and the maximum excitation wavelengths of bacteriorhodopsin(from 326 to 331 nm) occurres at a narrow concentration range of HCl(11.5-13.3 mmol/L). The transition process performs within 0 to 2s.

通过荧光光谱、吸收光谱和停流技术对酸化紫膜转变成蓝膜变化过程的跟踪研究表明，视黄醛特征峰的递变(570→605nm)以及蛋白质荧光最大发射波长的递变(326→331nm)都是在狭窄的HCl浓度范围(11.5-13.3mmol／L)内同步进行的，该转变过程在0-2s之内极快完成。

Label with LC-Red640 and LC-Red750 two are aimed at above all 112 code with 158 child detect 5 ′ carry bougie, corresponding anchor decides bougie to be labelled by fluorescence in 3 ′ end, circulate quickly from inside DNA of person gene group again PCR is distinctive the enlarge that make thing adds part of ApoE gene 265bP and with distinctive bougie cross hind, cause fluorescent resonance energy to transfer, the occurrence that passes different source cross and fault match alkaline base the fusible parameter of the type will finish feral model divide with choppy gene model.

首先以LC-Red640和LC-Red750标记两条针对112和158位编码子的检测探针5′端，相应的锚定探针在3′端被荧光标记，再从人基因组DNA中快速循环PCR特异引物扩增apoE基因265bP片段并和特异探针杂交后，引发荧光共振能量转移，通过异源杂交的出现和错配碱基的类型的熔解参数来完成野生型和突变基因分型。

Er concentration.2. With the analysis of the optical parameters calculated by Judd-Ofelt theory and the lifetime of ~4I_(13/2) of Er gotten in experiment, we investigated Y dopings dispertion effect and influence to the environment of Er .3. In the research of Er/Yb/Al co-doped laser glass, we fixed the Er:Yb at 1:6 and varied the Al concentration.

我们利用Judd-Ofelt理论，计算得到了Er/Y共掺玻璃的Judd-Ofelt强度系数、光谱质量因子、自发辐射弛豫率和能级寿命等光谱参数，并且给出了不同Y掺杂浓度下Er~(3 )离子的荧光量子效率；结合Er~(3 )离子~4I_(13/2)能级的荧光寿命，深入探讨了掺Y对高浓度Er~(3 )离子在硼硅酸盐玻璃中分布的弥散作用和对配位场环境的影响。

In our research, marked autologous fluorescent blood red cell is immitted into SD-rat body and the whole process is shown and recorded by microscope image system.

利用做过荧光标记的自身红细胞注入SD大鼠体内，通过显微图象系统将整个过程以视频信号的形式存贮，然后利用基于视频图象的采集卡，将流速变化过程回放采样，得到暗视场下的荧光图象，利用图象分析和处理的方法，测定血流速度。

But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊！不用提了。提到肉，真是糟透了。