荧光的

Methods KC and FE of tympanic membrane were cultured on the surface of CCM prepared by torrefaction, then the cells biological characteristics were detected by immunofluorescence stain of keratin and vimentin, and the cells proliferative activity were identified by immunofluorescence stain of PCNA.

方法热干法制备CCM，传代的大鼠鼓膜上皮KC和FB分别接种于培养板及CCM表面，通过角蛋白、细胞膜波形蛋白免疫荧光染色鉴定细胞特异性，通过细胞核增殖抗原(proliferating Cell nuclear antigen， PCNA)免疫荧光染色测定膜上细胞的增殖能力，通过细胞培养液上清的经脯氨酸测定评价对FB胶原分泌功能的影响。

The change of agglutinating activity in different temperature and pH indicated that PML had no hemagglutinating activity at 80℃. The effect of chemical modification of amino acid residues upon hemagglutination was investigated. There was a definite decrease of fluorescence intensity which corresponds to the oxidation of tryptophan.

研究了不同温度、pH和变性剂对PML凝血活性的影响和荧光光谱的变化，当温度达80℃时，活性完全丧失；pH为4—9对活性影响不大，pH低于2和pH高于12时，大黑花芸豆凝集素的凝血活性大部分丧失；高温和强碱对荧光光谱有较大影响，表明PML对高温，强碱的耐受性不强。

The UV-visible spectra of oxidized and reduced F28Y and F106Y were in agreement with the wild NGB, but the oxidized F28Y＇s Sorer band had a 2 nm blue shift, which suggested that the two mutants still remain hexa-coordinated form.

氧化型和还原型F28Y及F106Y的紫外-可见吸收光谱与野生型相似，仅氧化型F28Y的Soret带有2nm的蓝移，说明这两种突变体蛋白仍保持六配位形式。F28Y的荧光最大发射峰明显红移（340nm→347nm），表明其荧光基团更加暴露于极性环境中。

One of them was designed as a potential fluorescent probe to detect aldehyde group reductase in vivo, with a disctinct wavelength movement between the on-off states.

我们对其中一个分子进行结构修饰后，发展了一种基于ICT效应的荧光比率型荧光探针，它的on-off状态之间的波长变化达到了26 nm，今后有望用于体内醛基还原酶的检测。

08 Ns using deconvolution from the combination of the phase plane method and iterative convolution method.

通过相平面法和迭代卷积法相结合的解卷积处理，测得乙醛40％水溶液的真实荧光寿命为（7.25±0.08）ns，表明这是一种采用纳秒量级的仪器测量纳秒甚至亚纳秒荧光寿命的有效方法

It was shown that the fluorescence spectra of fluorescein in the gels had a red shift in contrast to those in the solution of diphenyl ether and that the gelation thime decreased with increasing the concentration of BMDM.

研究结果发现，与荧光素在二苯醚溶液中的荧光光谱相比，在凝胶中出现了 10～25nm的红移；而且发现分子凝胶的凝胶化时间随着BMDM浓度的增加而减小。

It grows louder and more distinct, until you round a corner and see a fairyland of dancing flashes , as the burnished copper catches the light of innumerable lamps and braziers.

而且越来越响越明显，直到你绕过一个角落看到荧光闪闪的仙境，这荧光是抛光过的铜器撞击到灯和火盆的无数束光形成的。

The dopping of Ce would not lead to new PL peaks, but an appropriate Ce dopping could increase the PL intensity.

铈的掺杂没有引发新的荧光现象，适量铈的掺杂能够增强催化剂样品的荧光强度。

In mouse peritoneal macrophages. Methods Mouse peritoneal macrophages were cultured in the presence of heat-killed yeast-phase PM for 24 h, and the average fluorescence intensity of TLR-2, TLR-4, and Dectin-1 in the macrophages was detected using flow cytometry. Fluorescent staining of the macrophages was performed to observe the fluorescence of TLR-2, TLR-4, and Dectin-1 with confocal microscopy. TNF-α mRNA in the cell culture supernatant was measured with real-time PCR, and TNF-α protein detected using enzyme-linked immunosorbent assay.

马尔尼菲青霉酵母相菌液与小鼠腹腔巨噬细胞共培养24h，采用流式细胞技术检测巨噬细胞TLR-2、TLR-4及Dectin-1的平均荧光强度；共聚焦显微镜观察荧光染色的受体；ELISA法测定培养液上清中TNF-α的浓度；Real time PCR检测不同时间段TNF-α的mRNA表达。

Objective To study the effects of heat-killed Penicillium marneffei on the expressions of toll-like receptor-4 (TLR-4),toll-like receptor-2 (TLR-2) and dendritic cell associated C-type lectin-1 (Dectin-1)and the production of the proinflammatory cytokine tumor necrosis factor-α.

办法马尔尼菲青霉酵母相菌液与小鼠腹腔巨噬细胞共培养24h，采用流式细胞技术检测巨噬细胞TLR-2、TLR-4及Dectin-1的平均荧光强度；共聚焦显微镜观察荧光染色的受体；ELISA法测定培养液上清中TNF-α的浓度；Real time PCR检测不同时间段TNF-α的mRNA表达。

But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊！不用提了。提到肉，真是糟透了。