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Induced activation of Rac1,Cdc42 signal transduction pathway, monitoring the transfer of living cells activated sheet pseudopodium emerged and filopodia obvious morphological changes,such as measured values or fluorescence imaging of fluorescence intensity over time to continuously reduce until almost see the determination or observation.

诱导激活的Rac1、Cdc42信号转导通路,监测到转染激活的活细胞中出现片状伪足和丝状伪足等明显的形态变化,测定荧光值或者成像的荧光强度随着时间推移不断降低,直至几乎测定或观察不到。

The difference between the test groups and the control group was statistically significant. It could be deduced that all the insecticides tested in this experiment damaged the spiders′ intestinal mucosa, compromising its function as a barrier and letting large molecules such as FTIC-D more easily penetrate. Among the insecticides, pyrethrin and Bt preparation are bio-insecticides, and the FITC-D concentrations of the spiders exposed to these two bio-insecticides were more than those of the spiders exposed to the chemical insecticides imidacloprid and dipterex. The difference between the bio-insecticide groups and the imidacloprid group was significant, but there was no significant difference between the bio-insecticide groups and the dipterex group.

结果表明:4种杀虫剂处理后拟环纹豹蛛血液中的荧光素浓度远高于对照组,各个处理组拟环纹豹蛛血液样本中的荧光素浓度均与对照组存在极显著差异,可见4种杀虫剂均将对拟环纹豹蛛肠黏膜的屏障功能造成损伤,导致大分子物质易于通过;其中1.5%除虫菊素水乳剂三保奇花和Bt制剂强杀处理后拟环纹豹蛛中肠黏膜的通透性高于仿烟碱类化学杀虫剂吡虫啉处理组,差异达到显著水平,但与有机磷杀虫剂敌百虫处理组差异不显著。

Its characteristic band is sensitive to the polarity of environment. It was found that the semilogarithmic plot of λ versus surfactant concentration exhibited two inflection points. The first inflection point corresponded to the critical micelle concentration, and the second inflection point C(subscript cm addressed the critical point reaching dense structure in the micellar core.

由于它的荧光特征波长对环境极性的敏感性,其荧光发射波长-表面活性剂浓度(λ-1gc)曲线出现了2个浓度相距较大的转折点,第一个转折点对应临界胶团浓度,第二个转折点C则表征胶团结构达到较为密实的临界点。

Dichlo-5- carboxylic acid Rhodamine is a kind of bright flurescent using xanthene as scaffold, whose aryl-H was substituted with a variety of groups. Compared with any other rhodamine, they can increase or decrese emissive absorbance in most limitation.

4,7,-二氯-5(和6)-羧酸罗丹明荧光染料是一种带有羧基官能团的荧光染料,作为一种特殊的抗体染色剂,在生物,医药方面具有广泛的应用,能标记DNA和蛋白质等大分子,这些性能被广泛应用于DNA分离和定序的研究中。

Fluorescence in situ hybridization with 16S rRNA-targeted oligonucleotide probes was applied for analyzing the community of iron bacteria in wastewater from the Shengli Oil Field. Four 16S rRNA probes were designed, which were highly specific to iron bacteria Leptothrix, Sphaerotilus, Leptospirillum ferrooxidans, Leptospirillum ferriphilum and Acidithiobacillus spp.

根据油田外排水中常见铁细菌的16SrRNA特异性保守序列,设计和合成了4种荧光探针,利用荧光原位杂交方法,分别利用它们对胜利油田孤岛采油厂、胜利采油厂1号和2号综合处理站外排水中存在的铁细菌的种类和数量进行检测,分析了各外排水中铁细菌的群落结构。

For the purpose of realizing the LITVF technique in the visible region of the spectrum, BaCl was selected as the seeding molecula, and the spectrum feature of the BaCl in the premixed laminar flame were investigated experimentally.

在对激光诱导热助振动荧光光谱理论分析的基础上,本文以较大的篇幅讨论了稳定预混层流火焰中BaCl分子的热助振动荧光光谱的实验研究结果和具体实验装置。

However, the Cry1Ac-GFP fusion protein only accreted with peritrophic membrane of resistant strain and showed fluorescence, which suggested that the binding of peritrophic membrane with Cry1Ac toxin protein may result in resistance of larvae of H. armigera to Cry1Ac insecticidal crystal proteins.

比较Cry1Ac杀虫蛋白敏感和抗性棉铃虫幼虫中肠的发光部位,敏感棉铃虫幼虫的中肠围食膜已经消失,肠壁细胞发出强烈的荧光,而抗性棉铃虫的围食膜较健全并发出荧光。

We also studied the spectra of the dye and determined the fluorescence quantum yields in different solvents. Also discussed its photostability and sensitivity to different pH.

研究了染料在不同溶剂中的吸收光谱和荧光光谱,测定了染料在不同溶剂中的荧光量子产率,并对染料的光稳定性及对pH值的敏感性进行了探讨。

Utilizing the intense catalysis of metal ions to peroxide oxygenating rhodanine, a catalytic fluorescence method of using the new type rhodanine to determine trace iron, cobalt, copper, tin and antimony was established.

利用痕量金属离子对过氧化氢氧化若丹宁具有强烈催化作用的性质,建立了一系列利用新型若丹宁物质催化荧光法测定痕量铁、钴、铜、锡和锑的新方法;较系统研究了系列若丹宁衍生物在催化荧光法测定金属离子的各影响因素,确定了反应的最佳条件,计算了动力学参数。

In chapter 5,several novel fluorescent dyes based on perylene tetracarboxylic diimides were prepared by a catalytic nucleophilic addition of an activated methyl group to carbonyl group.

第四章:以无水氯化锌为催化剂,将含活泼甲基的2-甲基喹啉连接到PDI单元的酰亚胺N的位置,合成了两个含PDI单元和硼氟荧光体单元的新颖荧光染料。

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