荧光的

With the same method and under the same condition, we get the bibulosity . alcoholism blood of fluorescence spectra of whole blood and erythrocyte. It is discovered that during the course when rabbits change from unconscious state to conscious state, in the whole blood and erythrocyte of the same concentration, the changing rules of their spectra intensity are the same as the ones in the serious examination alcoholism, but only with a small range.

在同样方法和实验条件下得到了一般酒精中毒兔全血、红细胞混悬液的荧光光谱，结果表明：从酒精中毒至慢慢恢复的过程中，同一浓度的全血、红细胞溶液，其荧光强度的变化规律与严重酒精中毒时一致，只是变化幅度较小。

Third, sodium sulfoxylate formaldehyde is a kind of bleaching powder used in industry, which is prohibited from adding to food. In this part, we take it as an object to study, by using ion chromatography instrument which is equipped with autech DS-PLUS?

本章利用荧光光度法和紫外吸收分光光度法研究了核酸对环丙沙星-Tb~(3+)体系的荧光增强效应及其影响因素，探讨了其作用机理，提出了环丙沙星-Tb~(3+)配合物与核酸的作用模型，并建立了DNA的选择性测定方法，并将其运用于合成样与血清中DNA含量的测定。

All of these results confirmed that the quenching effect of sulfur atom. The distance between sulfur atom and chromophore played an important role in the self-quenching effect.

通过实验，进一步证实了硫原子对发光基团的荧光淬灭作用，说明二硫代酯与发光基团之间的距离对硫的荧光淬灭作用有很大的影响。

Methods］ A total RNA from 10 cases withovarian papillary serous cystadenocarcinoma and normal ovarian tissues were extracted by a single step method of gene chip. The cDNA retro?鄄transcribed from equal quantity mRNA derived from 10 cases with ovarian papillary serous cystadenocarcinoma and normal ovarian tissues. The cDNA strand was labeled with fluorescent Cy5 and Cy3(normal ovarian epithelial cells) as probes.

方法］ 采用基因芯片技术，按一步法分别抽提10例卵巢浆液性乳头状囊腺癌患者的癌组织和正常卵巢组织的总RNA并纯化mRNA；逆转录合成以Cy5荧光标记卵巢癌细胞和Cy3荧光标记正常卵巢上皮细胞的cDNA作为探针，在含有8 464点人类体细胞基因模板的表达谱cDNA芯片上进行杂交。

METHODS: The level of intracellular calcium of human microglia grown on coverslip,which was loaded by calcium-probe,Fluo-4,and then treated in various experimental processing,was detected by confocal microscopy with time resolution mode.The binding of gp120 to human microglia was determined with confocal microscopy or flow cytometry after treatment with gp120 and stained with anti-gp120-FITC antibody.Phosphorylation of ERK within human microglia with or without gp120 stimulation was analyzed with confocal microscopy following the direct immuno-staining with anti-phosphorylated ERK antibody.

用钙离子探针Fluo-4标记粘附在盖玻片上的人小胶质细胞，运用共聚焦显微镜以荧光强度为指标实时观察各种条件下的细胞内钙离子水平的变化；用gp120处理并用anti-gp120-FITC进行染色，运用共聚焦显微镜术和流式细胞术分析人小胶质细胞与gp120结合情况；用抗磷酸化ERK 抗体免疫荧光方法进行染色，运用共聚焦显微镜术和流式细胞术进行ERK磷酸化水平分析。

With conventional PCR primer pair XBFF/R,the same 352bp bands appeared by amplifying all the DNA samples from the eight races,and no bands appeared by amplifying all the DNA samples from the 9 plant pathogen species(Cucumber powdery mildew,wheat yellow rust,wheat leaf rust,wheat stem rust,wheat scab,wheat sheath blight,wheat loose smut,maize sheath blight and maize head smut)and control.

3用常规PCR和实时荧光PCR两种方法对温室接种的小麦白粉病进行了检测，并在时间上与自然显症的观察进行了比较，结果表明：在温室条件下，小麦白粉病菌在接种后第5d时开始出现小麦白粉病病斑，而利用常规PCR引物XBFF/R可在接种第3d检测到小麦白粉病菌，比自然显症观察能够提前了2d检测到白粉病菌；实时荧光PCR法可在接种后1d即能检测到小麦白粉病菌，比自然显症观察能够提前了4d检测到白粉病菌，这可为白粉菌的预测或预警检测提供依据，更可为白粉病在流行前制定防治决策赢得宝贵时间。4。

After inserting the mutant fluorescence protein gene into the vector, the novel vaccinia virus vector p16H1FP and p16H2FP to express proteins as fusion products containing an additional amino-terminal stretch of six histidines were constructed.

我们用PCR扩出N-端融合6×His·Tag多克隆位点基因序列，插入p16表达载体，并在其多克隆位点下游装入带有巨细胞病毒启动子的突变型荧光蛋白基因，构建成两个读框的N-端融合6×His·Tag并带有荧光蛋白报告基因的分离型痘苗病毒表达载体。

The theoretical calculation indicates that the fluorescence efficiency of single mode is 5.83% and multimode is 1.75%.

理论计算表明：单模和多模的荧光效率分别为5.83%和1.75%，在此计算中单模的荧光效率比多模的高。

In order to establish a quantitative method in detecting HIV 1 proviral burden and viral load, 8E5 cell line and a recombinant RNA constructs were used as the HIV 1 proviral DNA and viral RNA external references, respectively.

以定量的HIV 1DNA和RNA为标准外参照，利用SYBRGreen荧光染料和GeneAmp5 70 0SequenceDetectionSystem (5 70 0系统)，建立了测定HIV 1的前病毒载量和病毒载量的荧光实时定量PCR技术。

In thisthesis our endeavor has been focused on the design of fluoresent sensors for sensinggroup 11 metal ions and phosphines －a very impotant type of organic molecule inthe industral and academic aspects.

三价磷的有机化合物由于其独特的配位能力已经吸引越来越多的人对它们进行深入研究，我们将三价磷受体单元同蒽荧光团有机地结合起来设计合成出下列新型含蒽荧光团双膦配体。

But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊！不用提了。提到肉，真是糟透了。