荧光的

Three expression vectors containing mutant gfp which were truncated from 3′ termine were constructed in the present paper.The mutation gfp with different length under the control by T 7 promoter were expressed in E. coli BL21 cells.

本文将突变型 gfp3′末端不同程度地截短，构建成表达载体，在大肠杆菌 BL 2 1中表达了不同长度的绿色荧光蛋白，当其生色基因中 65位 Ser点突变成 Thr，则 GFP的激发波由 396nm增至 4 70 nm，不用 UV激发，仅在自然光源下就可看到强烈的绿色荧光，发光强度增加 6倍，若从 3′端截短 12个氨基酸密码，对发光无影响，但截短 75个氨基酸密码，则激发波长降到 360 nm，发光强度减少 12倍。

At the same time, the intensity ratio of the ICT fluorescence to the LE fluorescence, I〓/I〓, shows a "bell"-shaped variation with increasing CTAB concentration The intensity ratio was found to level off after the CMC of CTAB Together with the data from UV-Vis absorption spectroscopy, electric conductance measurements and the control experiments with tetramethylammonium bromide, it was concluded that the"bell"-shaped variation in the intensity ratio before CMC is due to the formation of premicelle aggregates consisting of ICT fluorophore and CTAB.

此时，ICT荧光强度与LE荧光强度之比，I〓/I〓随CTAB浓度增大而呈&钟形&变化。当CTAB浓度高于CMC时，I〓/I〓为一常数。根据紫外-可见吸收光谱，电导以及四甲基溴化铵对照实验的结果可知，当CTAB浓度低于其CMC时，I〓/I〓的&钟形&变化是由于长链对二烷基氨基苯甲酸与CTAB组成的胶束前簇集体形成所致。

The reaction involving the oxidation of thiamine by Hg2- in basic solutions to fluorescent thiochrome was studied.

目前国产的荧光光度计上几乎没有配置流通池，这给荧光速率法的研究带来了很大的不便，尤其是在研究快反应时，容易产生

The gene chip detecting methylation changes of 3 CpG in promoter region of p16 gene was constructed by designing a pair of probes which contain one methylated and one unmethylated probes. This pair of probes was used to detect 3 consecutive sites of CpG island in p16 gene.

设计1组特殊荧光标记探针以构建1种检测p16基因启动子区3个CpG位点甲基化改变的芯片，特殊荧光标记探针包括1对非甲基化探针和甲基化探针，检测相邻的3个CpG位点甲基化的程度。

We choose the solid phase extration to deal with the biolog...

本文采用固相萃取法对叶酸血浆样品进行前处理，首次应用大孔吸附树脂作为萃取固定相，同时，还研究了叶酸的光化学行为，发现叶酸本身有弱荧光，在一定的反应介质中，经光化学诱导，可产生较强的荧光产物，从而明显改善检测灵敏度。

Just for this purpose, the research of QDs-BSA conjugates was made in this work.

这里正是着重从这一角度入手，以牛血清白蛋白(bovine serum albumin， BSA)为代表，研究了生物分子修饰QDs的最佳反应条件，发现QDs经BSA修饰后荧光明显增强，并对荧光增强的原因做了比较系统的探讨。

To explore the existence and dynamic changes of the Golgi body-like structure in the log-phase and encysting trophozoites (6, 12, 18 hours) of Giardia lamblia, these trophozoites were stained with C6-NBD ceramide, a specific marker for Golgi body in eukaryotic cells, and then they were observed with fluorescence microscopy.

目的 探讨类高尔基体结构在蓝氏贾第鞭毛虫滋养体和成囊不同时段的存在情况及其动态变化机理。方法用可特异性标记真核细胞高尔基体的荧光染料C6-NBD神经酰胺，标记有活性的蓝氏贾第鞭毛虫滋养体及成囊6、12、18h虫体，并用荧光显微镜观察。

The interaction of human serum albumin and folic acid was studied using fluorescence spectroscopy, UV absorption and synchronous fluorescence spectroscopy in the pH 7.4 Tris-HCl buffer system at different temperatures.

在不同温度下的pH 7.4的Tris-HCl缓冲溶液体系中，采用荧光光谱、紫外吸收光谱和同步荧光光谱研究了人血清蛋白与叶酸的相互作用。

Fluorescence spectra, absorption spectra and protonation properties of Chinese herbal medicine—Cortex Fraxini and its effective components—aesculin and aesculetin have been studied in this thesis. Based on the spectroscopic properties of aesculin and aesculetin at different pH values of their water solutions, a fluorescence method for the determination of aesculin and aesculetin in Cortex Fraxini was developed.

本文详细研究了中药秦皮及其有效成分秦皮甲素和秦皮乙素的荧光光谱与吸收光谱，深入探讨了秦皮甲素和秦皮乙素的光谱性质和质子化作用，制定了秦皮药材中秦皮甲素和秦皮乙素的荧光分析方法。

Advantages of specific coupling between biotin and avidin or streptavidin allow firefly luciferase to be immobilized on solid support coated with avidin or streptavidin, so that more flexible and convenient methods of luminescence assay will be available in application.

利用生物素与配体亲和素或链霉亲和素的特异性耦合，可以将荧光素酶固定到亲和素或链霉亲和素包被的磁珠上，从而使荧光检测的应用更加灵活和方便。

But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊！不用提了。提到肉，真是糟透了。