荧光的

It would be helpful to understand the bioluminescent essence and origin of the firefly and other bioluminescence organism.

这将有助于理解萤火虫及其它生物自发荧光的本质和起源进化过程。

In addition, the character of the anti-Stokes fluorescence decay time was observed.

同时，我们观察了反斯托克斯荧光的时间特性。

It demonstrates that the illuminant substance of skin won't affect the measurement of porphyrin.

由此可见皮肤中发光物质不会影响卟啉荧光的探测。

The results also indicated that the mode of binding of the complex to DNA was intercalation action.

分析结果表明，THP以嵌入方式与DNA发生相互作用，并且生成了不发荧光的络合物。

This paper reports the sensitizing effect on the fluorescence of Mo morin complex by CTMAB.

研究了十六烷基三甲基溴化铵对钼－桑木色素络合物荧光的增敏作用。

The antibody is bound to an inert polymer support ,then exposed to reacts with the antigen at a different epitope is added. The second antibody used is one that has an enzyme attached to it that coverts a colorless or nonfluorescent substrate into a colored or fluorescent product. The amount of second antibody bound, and hence the amount of protein antigen present in the original sample, is determined by quantification of the intensity of color or fluorescence produced.

将抗体结合在一惰性聚合物载体上，然后与样品接触，洗脱未经结合的蛋白质加入与抗原其他表位也可以结合的另一种抗体，在所用的第二抗体上结合有酶，此酶可把无色的或无荧光的底物转化有色或显示荧光的产物。

DHCC Constance in the chemistry belongs to the onlighted Cyclopentanoperhydrophenanthrene when it reacted together with the acid Constance it could transform into the fluorescence phenol.we did the flouric determination for the DHCC, in which treated by the ethanol-sulfuric acid or acetic anhydride-methylene chloride-sulfuric acid, acquired standard curve in the 0-40μg/ml presented the liner relet...

DHCC类物质在化学上属于不发光的环戎烷多氢菲类，但与酸性物质一起反应时，可转化为产生荧光的酚类。用乙醇硫—酸或乙酸酐—二氯甲烷—硫酸处理后的DHCC进行荧光检测，获得标准曲线0—40μg/ml范围内呈线性关系，检出的最高灵敏度可达300ng/ml。该方法可用于分离后的DHCC及单一种DHCC代谢物的定性及定量分析。

Cells were immediately fixed and processed for immuno?uorescence and tested for translocation ofβ-catenin.β-catenin and target genes of Wnt/β-catenin signaling COX-2, cyclinD1, c-fos, c-Jun mRNA expression were examined by RT-PCR. Saos-2 osteoblastic cells were co-transfected with a wild type or mutant Tcf luciferase reporter gene and Renilla luciferase plasmid. Posttransfection cells were subjected to 1 hour of 12% elongation.

作用1h、2h和4h后，用免疫荧光的方法检测β-catenin的表达和在细胞上定位的变化；用半定量RT-PCR检测β-catenin mRNA表达的变化；用质粒转染的方法检测荧光素酶报告基因Tcf转录的情况；用半定量RT-PCR检测Wnt/β-catenin通路下游基因COX-2，cyclinD1， c-fos， c-Jun mRNA表达的情况；用免疫共沉淀的方法检测β-catenin和E-Cadherin的结合情况。

As a result, dual fluorescence was observed in non-polar solvents. Long-wavelength emission was found to consist of two components:+D||A" exciplexes resulted by "harpooning effect" and +D^^A" exciplexes stablized by solvation.

研究结果表明：合成的化合物在非极性溶剂中有双重荧光的稳态发射行为；双重荧光中的长波长发射经时间分辨光谱研究为双指数发射衰减；其中的长寿命衰减是由于该类化合物在非极性溶剂中激发后经过分子内电荷转移形成了激基复合物所致，而产生该激基复合物的直接原因是由于在非极性溶剂中的&harpooning effect&所致，短寿命则来自于该类化合物激发后在溶液中发生了分子内电荷转移的直接衰减。

Green fluorescent protein gene was conjugated to the 3 end of the PAP gene in order to screen easily of the transgenic cotton plants. The combined gene was cloned into plant expression vector pBI121 and then transformed. About 5000 seeds of the transgenic cotton were obtained and the some seedlings of the transgenic cotton could give a bright green fluorescence in the dark condition when the cotton seedlings were irradiated with ultraviolet rays. This result reflected that the GFP gene was expressed in the transgenic cotton; dot-blot result also demonstrated that PAP gene was exactly transformed into the cotton. The capacity of the Verticillium dahliae-resistance is being analyzed.

为了便于转基因棉花后代的筛选，在 PAP基因的 3'端融入了绿色荧光蛋白GFP）基因，然后将融合基因克隆在植物表达载体PBI 121上，再进行遗传转化，得转基因棉花种子5000余粒，将种子播种长到于叶展开时，先在黑暗中用紫外灯照射，查找表现绿色荧光的幼苗，然后再用地高辛标记的PAP基因特异性探针对这些棉花进行点杂交，最后发现有8株棉花表现阳性反应，说明PAP基因的确己经转到了棉花的基因组中，其棉花黄萎病的抗性鉴定正在进行之中。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。