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The cardiomyocyte viability were detected by MTT assay for calculating the survival rate and the inhibitory rate of the virus. Myocardial F-actin and VEGF were analyzed by immunofluorescent staining with confocal microscopy. Mean fluorescent intensity of F-actin and VEGF were determined by flow cytometry.Results There is no toxicity on cardiomyocytes when PD is at concentration of 0.02 mmol/L and 0.2 mmol/L while there is toxicity at concentration of 2 mmol/L. CVB3 could reduce the viability of cardiomyocytes, depolymerize F-actin cytoskeleton and reorganize VEGF protein.

体外培养心肌细胞,用100 TCID50柯萨奇B3病毒(CVB3)感染心肌细胞,建立病毒性心肌炎实验模型,然后分别用0.02mmol/L、0.2mmol/L、2mmol/L三种不同浓度的PD处理感染CVB3病毒的心肌细胞,观察心肌细胞的形态和搏动,用细胞免疫荧光技术标记纤维状肌动蛋白和VEGF蛋白,四唑蓝比色法测定心肌细胞活性和病毒抑制率,流式细胞仪检测各组心肌细胞VEGF蛋白和F-actin平均荧光强度。

Results The in vitro coupled bacterial luciferase:FMN-NADH oxidoreductase bioluminescent system was: 1mL crude extract, 27 mmol/L Dodecane 100 μL, 10 mmol/L FMN-Na 0.5 μL and 0.14 mmol/L NADH 300 μL.

利用从鳆发光杆菌提取并经部分纯化的荧光素酶和FMN-NADH氧化还原酶,通过优化体系中各底物的添加量,实现荧光素酶的体外发光。

We have synthesized fourdioxotetraamine ligand appended with fluorenyl group and their transition metal complexes.The crystal structures of the complexes were determined by x-ray diffraction. Stability,electrochemistry and switch properties in aqueous solution was studied by pH-potentiometricand fluorimetric titration as well as cyclic voltammetry.

合成了带侧链荧光基团—芴的二氧四胺配体及其过渡金属配合物,测定了过渡金属配合物的晶体结构,研究了他们在水溶液中的稳定性、电化学性质和荧光开关性质。

With fluorescin as reporter molecules, the distribution and elimination of the liposome were tested in vivo in mice.

导向脂质体可以明显减慢荧光素钠在小鼠体内的吸收与消除的过程,并明显提高荧光素钠在肝组织中的分布含量。

The dimerization of homovanillic acid by hydrogen peroxide in the presence of manganese tetrasulfonatophthalocyanine has been adapted to the determination of hydrogen peroxide in rainwater.

在四磺基锰酞菁存在下,高香草酸与过氧化氢作用可形成强荧光二聚体,由此建立的测定水溶液中过氧化氢的荧光方法可用于本地区雨水样品的分析。

In 1888, when he was working at Heidelberg under Quincke, Lenard had done his first work with cathode rays.

莱纳德很快对磷光和荧光现象发生了兴趣,这种在黑暗中发出的神秘的微弱亮光,从小就吸引了莱纳德,他曾和他的同学加热氟晶体让它发出荧光。

At 7 and 14 days following transection of masseteric nerve through which Fluorogold was applied to identify the Me5 neurons innervating masseter, brain sections were immunohistochemically processed to detect the three Trk isoforms in FG-labeled Me5 neurons.

通过大鼠咬肌神经给予荧光金,标记支配咬肌的Me5神经元;分别于切断咬肌神经后7和14 d对脑切片进行免疫组织化学染色并观察荧光金标记的Me5神经元表达的三种Trk受体。

Nuclear translocation of protein PDX-1 in different groups: Indirect immunofluorescence showed PDX-1 was mainly located in cytosolic slightly in the basical station, while translocated to nucleoplasmic greatly followed by stimulated with 16.7mM glucose.

为量化这种核转位程度,每组计数100个细胞并分别记数有荧光信号在核内表达的细胞数N及荧光信号在核周围表达的细胞核数C,比较各组的N/C值。

The spectrum of phonon energy can be adjusted by varying the content of SiO2 in the oxyfluoride glass and glass ceramics, which affects the upconverted fluorescence emission of doped ions.

研究了基质结构和样品环境温度与电声子相互作用的联系以及声子变化对于荧光辐射产生的影响。发现改变基质材料的SiO2含量能影响蓝色上转换荧光效率。

Fluorophore-assisted carbohydrate electrophoresis was used to analyse pectic oligosaccharides. The influence of different factors on the separation of oligosaccharides by FACE was investigated.

荧光辅助糖电泳是首先用荧光衍生化试剂对糖类分子的还原端进行衍生化标记,然后在一定浓度的聚丙烯酰胺凝胶上进行分离的分析方法,可同时分析中性糖与酸性糖。

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