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Fluorescence in situ hybridization was carried out to confirm the integration of HBV DNA into male pronucleus and its replication with cell division in embryonic development.(Reverse transcriptase polymerase chain reaction,RT-PCR) and immunofluoresence assay were performed to observe the expression of the HBV gene in two-cell stage.

材料与方法:成熟雄鼠麻醉后双侧睾丸注射经脂质体DOSPER包裹的HBV质粒,手术后雄鼠与超排雌鼠合笼交配,用荧光原位杂变(Fluorescence in situ hybridization,FISH)分别检测单细胞胚和二细胞胚间期核中HBV DNA的存在与复制,用逆转录聚含酶链反应(Reverse transcriptase-polymerase chain reaction,RT-PCR)和免疫荧光方法检测HBV基因在二细胞胚胎中的表达。

A simple and highly sensitive new kinetic catalytic fluorimetric method was proposed for the determination of trace chromium,based on the catalytic effect of trace amounts of chromium on the oxidation of Pyronine Y by hydrogen peroxide in acetic acid-sodium acetate buffer medium leading to a decrease in the fluorescence intensity.

在HAc-NaAc缓冲体系中,痕量铬对过氧化氢氧化吡咯红 Y的氧化还原反应有催化作用,使吡咯红 Y荧光减弱,据此建立了催化荧光法测定痕量铬的新方法,同时考察了该催化反应的最优条件及动力学性质。

The method was successfully used to quantitate the virus load in tiss...

最后用建立的方法对108份临床样品进行检测并同时进行病毒分离,荧光定量PCR方法检测出73份阳性样品且与病毒分离的符合率为100%,而常规RT-PCR只检测出54份阳性样品,表明本荧光定量RT-PCR法在检测猪瘟病料上具有潜在的应用价值。

Based on the β-cyclodextrins' fluorescence enhancement to resorcinol, a new way of fluorescence analysis for resorcinol is presented in this paper.

利用β-环糊精对间苯二酚的包络作用,增敏间苯二酚的荧光强度,提出一种测定环境中间苯二酚的荧光检测新技术。

The time-resolved technique currently used, is generally utilized to improve selectivity by adjusting delay sampling time. It is effective for eliminating the interference from signals with shorter lifetime to that with longer lifetime, but the revers is invalid.

目前所使用的时间分辨荧光光谱分析技术,一般都是通过延迟一定时间后再对样品信号采样,这对于消除短寿命信号对长寿命荧光信号的干扰非常有效,但不能消除长寿命信号对短寿命信号的干扰。

In pH value 6.40 Clark-Lubs buffer solution, the fluorescence intensity of safranine T and sodium dodecyl benzene sulfonatewas determined. After adding organophosphorus pesticides, the fluorescence intensity was determined. Based on this, a new fast and simple method for determination of the organophosphorus pesticides total-residues was developed.

方法]在pH值6.40的Clark-Lubs缓冲溶液中,加入碱性藏花红与一定浓度的十二烷基苯磺酸钠,测定荧光强度,再加入有机磷农药,检测荧光强度变化,据此建立测定有机磷农药残留总量的新方法。

In this thesis, we extended study of controllable synthesis of cadmium selenide in a novel "green" way, fabrication of various CdSe core-shell structural composite QDs and study of the basic applications of the those composite QDs with excellent fluorescent effect in biomedical labeling, imaging and DNA biosensors as a new type of fluorescent probes .

本论文主要研究了制备CdSe纳米量子点的新型绿色制备工艺,构筑了多种核-壳结构复合量子点材料,并初步研究了利用复合量子点材料独特的荧光效应作为一种新型荧光探针,在生物标记成像和DNA生物传感器中的应用。

Objective To establish a human cervical carcinoma cell line with stable expression of green fluorescent protein and the fluorescent-labeled implanted tumor model, and compare the detection methods of tumor growth by optical imaging system and slide caliper.

目的 建立稳定表达绿色荧光蛋白的人宫颈癌细胞系,建立移植瘤模型并比较移植模型肿瘤生长的荧光分析和卡尺测量的优缺点。

The fluorescence properties of DAKGM solutions were studied in detail.The fluorescence properties of DAKGM solutions were studied via steady fluorescence spectra. Two emission peaks appeared at 425 and 465 nm, respectively.

过渡金属离子Fe3+和Cu2+能显著降低KGM的荧光强度,根据Stern-Volmer方程得到了Fe3+和Cu2+对KGM的淬灭常数分别为203.8 L/mol和171.0 L/mol.3、系统地研究了醛基葡甘聚糖溶液的荧光光谱特性。

The spectral performance of a self-Raman-active material (Nd:SrMoO4 crystal) was investigated. Its stimulated Raman scattering, absorption spectra, luminescence lifetime, and infrared emission under 808 nm LD excitation were measured at room temperature.

通过拉曼散射光谱,吸收光谱,荧光发射寿命和808 nm LD激发下的红外荧光光谱的实验测量,系统研究了Nd3+:SrMoO4晶体的自受激拉曼光谱性质。

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