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In order to further confirm its subcellular localization , pYES2.0-MxIRT1::GFP with a inducible promoter was constructed and then transformed into the yeast strain by LiAc method. We observed the cells under the fluoroscope and laser confocal microscope through a method of Fm4-64, red and green fluorescent co-localized in plasma membrane.

根据结构预测MxIRT1是一种膜蛋白,为进一步确认它的亚细胞定位,利用构建好的诱导型酵母表达载体pYES2.0-MxIRT1∷GFP,通过GFP与活细胞荧光膜染料FM4-64结合的方法在荧光显微镜和共聚焦激光显微镜下观察。

By fluorescence in situ hybridization. Strong fluorescence signals with the probes were observed in 20%~60% formamide concentrations.

的16S rRNA基因序列,设计荧光原位杂交检测博德特氏菌的寡核苷酸探针FW_iso_62和FW_iso_761,在20%~60%甲酰胺均有很强的荧光信号。

It is possible that the viscosity of glycerol increases or the orientation of PAHs promotes in magnetic field.

说明磁场有可能提高了荧光分子的定向作用或者提高了介质的粘度,从而使荧光分子的偏振度提高。

The experimental verification was accomplished.The whole system wassuccessfully applied to detect the fluorescent dye: Rhodamine B and Fluorescein,with certain sensitivity and linearity. The "light trap" structure has proved effective inreducing the background noise to some extent.

进行了实验验证工作:将构建的检测系统用于对荧光染料罗丹明B以及荧光素的检测,达到了一定的检测限以及线性度,并且验证了"光阱"结构能够在一定程度上降低背景噪声。

RLuciferase/Luciferin system, a new method to detect the MAPK activity in fungus, was founded and proved to be effective and perspective in the study of MAPK pathway in microorganism.

建立了一套切实可行的检测MAPK活性的荧光素酶/荧光素技术体系,并发现MAPK途径抑制剂U0126和CaM抑制剂TFP对MAPK活性都有显著的抑制作用。

Luciferin,as the light-carrier substance or the energy-transfer substance,plays an indispensability role on the luminescent system of luciferase in the biology illuminant.

在生物发光体内,荧光素作为直接释放光子的物质和能量的传递物质在荧光素酶发光体系中发挥着不可或缺的作用。

There are significantly differences on H_2O_2 levels between embryos in vivo and cultured in vitro on the early 2-cell stage and late 2-cell stage, but there are no significantly differences among embryos those on the 4-cell stage, morula and blastula using SPSS statistics software.

我们测量的结果是体内2-细胞早期、2-细胞晚期、4-细胞期、桑椹胚、囊胚胚胎的EB荧光密度分别是52.92,50.77,48.84,39.96和30.36;体外2-细胞早期、2-细胞晚期、4-细胞期、桑椹胚、囊胚胚胎的EB荧光密度分别是74.85,82.62,82.98,81.28和54.09。

This thesis is focus on the detection and processing of fluorescence signal based on TRFIA, the signal includes single signal and multidrop signal which is biological chip.

首先分析了荧光信号产生的机理,在此基础上考虑到荧光信号十分微弱的特点和在强噪声背景环境下检测,设计了基于数字BOXCAR的信号检测系统。

In Chapter 2, using 1,10-phenanthroline as the raw material, four ligands such as dipyrido[3,2-a:2',3'-c]phenazine,tetrapyrido[3,2-a:2',3'-c:3'',2''-h:2''',3'''-j]phenazine, 10,11-[1,4-naphtalendione] dipyrido [3,2,-a::2',3'-c] phenazine and 2-[4-hydroxylphenyl]imidazo [4,5-f] [1,10] phenanthroline were synthesized.

在此基础上研究配合物结构和性质上的变化,探索荧光指示剂结构与性质内在联系的规律,为光纤氧传感器提供荧光效率高、化学稳定性好的指示剂。

In the work, the laser-behavior under exposure of Ar〓 laser was discussed. The kinetic process of photochemical reaction, intermediate, sensitive process of diazophthoquinon system were studied by laser fluorescence spectrum and laser induced fluorescence attenuation. The nonlinear relation between photodecomposition rate of the system and laser power and concentration of solution was investigated in detail.

论文也进行了感光组成物感激光性能的研究:利用激光荧光光谱及激光诱导荧光衰减法实现了实时探测邻叠氮萘醌型光敏剂感激光光化学过程、中间体的产生与结构以及增感作用的研究,该方法在国内外文献中未见有报道。

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但我们并不在乎沙场中的显露。

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啊!不用提了。提到肉,真是糟透了。

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Tristan ,我不知道把这信寄到哪里,也不知道你是否想收到它。