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The relationship between the molecular structure and fluorescence performance of reagents was discussed in brief, and the influence of the electron-supplying and electron-withdrawing groups to the fluorescence performance of carboxylphenylazo rhodanine ramifications was also studied.

简要探讨了分子结构与荧光性能的关系,讨论了供、吸电子基团对羧酸基偶氮若丹宁类衍生物荧光分析性能的影响。

The fourth part: The influence of various active groups on the fluorescence performance of aresenoxylphenylazo rhodanine ramifications was studied systematically to seek the fluorescence reagents of good analysis performance.

第四部分:系统研究了各种活性基团对含胂酸基偶氮若丹宁衍生物荧光性能的影响,以寻求具有优良分析性能的荧光试剂。

The fluorescence characteristic of aresenoxylphenylazo rhodanine and the influence of-F,-Cl,-NO〓 and -OCH〓 on the fluorescence analysis performance were studied.

首次研究了胂酸基偶氮若丹宁的荧光性质,系统研究了-F、-Cl、-NO〓、-OCH〓基团对其荧光分析性能的影响。

A method of high performance liquid chromatography with post-column derivatization and terbium sensitized fluorescence for simultaneous determination of residues of ciprofloxacin, norfloxacin and enrofloxacin in chicken was established, based on the effect that Tb3+ can sensibilize fluorescence of fluoroquinolones.

基于氟喹诺酮类药物与铽离子形成配合物后的荧光增强作用,建立了同时检测鸡肉中氟喹诺酮类药物环丙沙星、诺氟沙星和恩诺沙星残留的Tb3+增敏高效液相色谱柱后衍生荧光检测方法。

During the past 3 years, 2 independent experiments of sperm-mediated gene transfer in domestic silkworms were carried out respectively in 2000 and 2002 with the method direct injection of copulation thecae.

在同源重组绿色荧光蛋白表达载体pG350的基础上,以pMD18-T为质粒载体,用egfp基因替换gfp基因,构建了家蚕核型多角体病毒早期即刻蛋白基因启动子BmNPV-IE控制下的增强型绿色荧光蛋白表达载体pMD-Fib-IE-EGFP。

To detection of Tilletia controversa Kühn sensitively and accurately, real-time PCR systems were developed. The species-specific primer pair CQUTCK4/CQUTCK5 and probe CQUP1 were designed based on a selected specific fragment (1322 bp) specific for TCK, and the SYBR Green I and TaqMan quantitative PCR detection systems were established with optimized reaction conditions.

建立荧光定量PCR体系以准确灵敏的鉴定小麦黑穗菌(Tilletia controversa Kühn, TCK)根据筛选的TCK独有差异基因片段(1322bp)设计特异性引物对CQUTCK4/CQUTCK5和TaqMan探针CQUP1,建立SYBR GreenⅠ荧光染料法和Taq Man水解探针法定量PCR检测体系,并对体系进行优化。

Besides, the effect of some foreign ions on the fluorescence signals was investigated and the interference of Fe3+ was found, which was eliminated by adding triethanolamine.

考察了其他常见离子对粒子荧光性能的影响,其中Fe3+使荧光发生猝灭,用三乙醇胺可消除Fe3+的干扰。

Results: The OECs could be purified to about 80 percent purity by the method, identified by immunofluorescence of GFAP and P75 with hoechst; there were many fusiform, bipolar, tripolar cells and a few multipolar cells under a phase contrast microscope.

结果:通过差速贴壁法纯化,可获得纯度约为80%的嗅鞘细胞,并通过GFAP免疫荧光及P75和hoechst双重免疫荧光鉴定;相差显微镜下细胞形态以梭形、双极及三极细胞为主,有少量的多极细胞。

To improve the stability of small unilamellar vesicles in vitro, the permeability of SUV from soybean phosphatidylcholine coated by collagen and chitosan was studied respectively by using 5(6)-Carboxyflourescein5(6-CF as a fluorescence probe.

为了提高小单层脂质体(SmalUnilamelarVesicles,SUV)的体外稳定性,以5(6)-羧基荧光素(5(6)-CF)为荧光探针,研究了脂质体被胶原蛋白和壳聚糖这两种天然生物大分子包覆后的通透性。

In this thesis,new fluorescence carriers with good properties were synthesized and copolymerized under UV irradiation on the silanized glass or quartz disk and -the sensors prepared were studied.

本论文主要工作是合成新型具有可共聚端基双键、性能优良的荧光载体,将荧光载体与其它单体在紫外光照下共聚在经过处理的石英或普通玻璃上制成光极膜,而后研究其分析特性及应用。

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