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The method was based on the aromatic hydroxylation of terephthalate by hydroxyl radical, produced from the homolysis of peroxynitrite in weak acid media, to yield a highly fluorescent product, 2-hydroxy-terephthalate, which was then detected fluorimetrically.

具体做法是将ONOO〓在pH 5.0的缓冲液中转化为其共轭酸ONOOH,而ONOOH本身不稳定,均裂生成·OH,·OH进攻对苯二甲酸生成强荧光产物2-羟基对苯二甲酸,利用荧光分析法测定2-羟基对苯二甲酸,从而间接确定ONOO〓的含量。

Result:The hybridization signals of DNA Fiber-FISH were more clear than those of interphase FISH.

结果DNA Fiber-FISH所显示的荧光信号明显比间期核FISH显示的荧光信号易于辨认。

In this paper, The photophysical properties of intramolecular electron and charge transfer under different pressure are explored.

本文主要利用高压作为调控参量,利用吸收光谱、荧光光谱和荧光衰减作为主要的研究工具,研究分子内电子转移和电荷转移体系的光物理性质。

Methods The model of intravasation migration of cancer cell in vitro was used. The fibrosarcoma cell HT1080 was labelled with fluorescence, and the fluorescence guantities in 24-pore plate through collagen membrane and HUVEC single cells layer were detected in different time to eveluate the intravasation migration of cancer cell.

利用体外癌细胞向血管内游走模型,将纤维肉瘤细胞HT1080经过荧光标记,通过测定不同时间穿过胶原层及HUVEC单细胞层落到24孔板内的荧光量来定量评价瘤细胞血管内的游走情况。

An enzyme present in the cells of bioluminescent organisms that catalyzes the oxidation of luciferin.

荧光素酶存在于生物性发光组织的细胞中,催化荧光素氧化的酶

To 7 days after transduction, cells were observed and recorded with fluorescence microscope, the expression levels of report gene EGFP in tumor cells were examined by using flow cytometry and Western blotting, the expression of report genes luciferase was analyzed with luminometer to obtain the relative light units.

用荧光显微镜观察EGFP阳性细胞,流式细胞仪检测EGFP阳性细胞的百分率和单个细胞平均荧光强度,Western印迹检测EGFP蛋白表达的变化以比较各组病毒转导效率。

A highly sensitive fluorescence method for the determination of germanium based on the formation of Ge- morin complex has been developed.

在H3PO4介质中锗与桑色素反应生成配合物,使溶液的荧光明显增强,据此建立了测定微量锗的荧光分析新方法。

With Mab 2A3, the first positive cells were detected at the limb bud stage of the embryos. Distinguishable positive haemocytes with a diameter of about 7μm appeared in embryonic-nauplius. At the following nauplius I stage, configuration of haemocytes became more integrated and clear. Their diameters were almost similar to those of haemocytes from adult shrimp. With Mab 1H11, the first positive granular haemocytes were identified at the stage of embryonic-nauplius which existed in clusters with positive punctate fluorescence of granules distributed around the periphery of granular haemocytes.

通过对包括囊胚期、原肠胚期、肢芽期、膜内无节幼体期和无节幼体Ⅰ期在内的中国对虾胚胎的间接免疫荧光检测,最早在肢芽期胚胎中检测到2A3阳性信号;在膜内无节幼体期,2A3阳性血细胞平均直径约7μm,聚集成簇;在无节幼体Ⅰ期,血细胞形态更加完整清晰,平均直径已与成体对虾血细胞相似。1H11阳性大颗粒细胞最早在膜内无节幼体期胚胎中出现,这些细胞同样成簇存在,在其细胞质外围可见点状荧光。

In the present study, soil samples were scanned by NITON XLt920 field portable X-ray fluorescenceanalyzer, and the relationship between the X-ray fluorescence spectra and the concentration of Pb in soil was studied.

在实验室条件下,利用NITON XLt920型便携式X射线荧光光谱(field portable X-ray fluorescence, FPXRF)仪获取土壤样品的X射线荧光光谱数据,并采用偏最小二乘法建立土壤Pb含量的预测模型。

After the recombinant plasmid pAd-VP linearized with PacI transferred into HEK-293A cell,the recombinant virus was isolated and purified in HEK-293A cells by three times plaque purification.

RT-PCR 检测证明目的基因在 mRNA 水平上可有效表达;应用 O 型口蹄疫病毒标准阳性血清进行间接荧光抗体试验,在 rAd-VP 感染的 HEK-293A 细胞的胞质可见清晰荧光。

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