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Fluorescein isothiocyanate labeled polyclonal goat antihuman immunoglobulin antibody was added, and flow cytometer was used to detect bead-platelet-associated autoantibodies-antihuman immunoglobulin antibody complex. Results The fluorescene ratio of four monoclonal antibodies was significantly different (P.01) between the idiopathic thrombocytopenic purpura patients and either the non-ITP patients or the normal controls. If the upper limit of normal control was set as cutoff value, ratios of greater than 1.37, 1.24, 1.48 and 1.19 were considered positive for the four monoclonal antibodies respectively. The flow cytometric bead assay had an overall sensitivity of 73.17% and a specificity of 94.29%. The overall sensitivity was significantly higher (P.05) than that of modified indirect MAIPA and that of using single antibody.

结果 特发性血小板减少性紫痕组4种羊抗荧光强度比值与非ITP血小板减少组和正常对照组有显著性差异(P.01);若将ITP组患者4种羊抗荧光强度比值分别大于正常对照组上限1.37、1.24、1.48和1.19判断为阳性,则流式微球技术检测血小板特异性自身抗体的敏感性为73.2%,特异性为94.3%;4种羊抗联合检测总体敏感性明显高于改良间接单抗特异的血小板抗原固定试验(P.05),且大于各单个抗体检测敏感性。

Fluorescein isothiocyanate labeled polyclonal goat antihuman immunoglobulin antibody was added, and flow cytometer was used to detect bead-platelet-associated autoantibodies-antihuman immunoglobulin antibody complex. Results The fluorescene ratio of four monoclonal antibodies was significantly different (P .01) between the idiopathic thrombocytopenic purpura patients and either the non-ITP patients or the normal controls. If the upper limit of normal control was set as cutoff value, ratios of greater than 1.37, 1.24, 1.48, and 1.19 were considered positive for the four monoclonal antibodies respectively. The flow cytometric bead assay had an overall sensitivity of 73.17% and a specificity of 94.29%.

结果 特发性血小板减少性紫癜组4种单抗荧光强度比值与非ITP血小板减少组和正常对照组有显著性差异(P<0.01);若将ITP组患者4种单抗荧光强度比值分别大于正常对照组上限1.37、1.24、1.48和1.19判断为阳性,则流式微球技术检测血小板特异性自身抗体的敏感性为73.17%,特异性为94.3%;4种单抗联合检测总体敏感性明显高于改良间接单抗特异的血小板抗原固定试验(P<0.05,且大于各单个抗体检测敏感性。

Pn knot add antihsiangvoltage,minority carrierdifficult to infusion,therefore no, aglow..this kind ofavailoneself of infusion styleelectricity to aglow principium make the crystal diode of callled,appellative led led aglow color concerned in luminosity andfabricator led material ho craft,currentlywidelyused have hung , green , lan three plants..based on led operating tensions low (littlesless than 1.5-3v),neng initiative aglowand has at all events brightness,brightnessandemployable voltageaccommodate,in itself and endure buffets ,shocksresistances moves , ages long ( 10 wan hour),forasmuch in man- size display device,currently shang free frommore bring out mode and led bring out mode is equal to.

由于led工作电压低(仅沈阳led显示屏-3v),能主动发光且有一定亮度,亮度又能用电压调节,本身又耐冲击、抗振动、led荧光电子广告板长(10万小时),所以在大型的显示重庆led灯中,led荧光电子广告板尚无其他的显示方式与led显示方式匹敌。

In addition, the ability of the copolymer to detect different metal cations in aqueous solution was investigated.

研究了常见金属离子对聚合物荧光强度的影响,其中以Fe~(3+)对聚合物的荧光淬灭程度最大。

And the additive synergism of built up multi-component fluorescent brightener for polyester fiber was confirmed through several examples.

详细介绍了涤纶用荧光增白剂的主要品种及其使用方式,并通过几组实例证实了多组分荧光增白剂复配后对于涤纶的加和增效作用。

This exhibited that hER cDNA and GFP gene had been recombined into the yeast cell.

荧光显微镜下发现大量的绿色荧光颗粒,表明GFP基因在酵母细胞中成功表达。

Determination of napropamide by spectrofluorimetry LIU Wen-li (Department of Environmental Sciences, Taizhou University, Taizhou 317000, Zhejiang, China) Abstract: The effects of organic solvents and acidity on fluorescence intensity of napropamide were studied.

荧光光谱法检测除草剂萘氧丙草胺刘文莉(浙江省台州学院环境科学系,浙江台州 317000)摘要:研究了酸度、有机溶剂对萘氧丙草胺荧光强度的影响。

Methods and characteristics are described in this paper for fluorescence resonance energy transfer microscopy and fluorescence lifetime imaging microscopy.

本文叙述荧光共振能量转移显微术及荧光寿命成像显微术的原理、方法及特点。

Thus, the caspase-3-like activation can be real-time monitored by fluorescence resonance energy transfer analysis.

该质粒在植物细胞中可以表达出两端为青色荧光蛋白和黄色荧光蛋白的融合蛋白。

The assay, which was based on primers and TaqMan-MGB probes selected from highly conserved regions of the hemagglutinin gene of avain influenza virus subtype H5, was optimized in a reaction system and a condition to improve the sensitivity, specificity and accuracy. In addition, colone technology was used to develop a quantitative PCR format with virus copies amount.

针对H5亚型禽流感病毒血凝素基因保守区域设计特异性引物与TaqMan-MGB荧光探针,筛选并优化荧光定量RT-PCR反应体系与反应条件,用以提高方法的特异性、敏感性与准确性;并通过体外克隆技术建立病毒基因拷贝数进行定量分析。

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