荧光的

Pyronine B adheres to on the surface of CdS and prevents CdS aggregating, which leads to the decrease of fluorescence quantum yield of CdS. Fluorescence resonance energy transfer happens from CdS to Rhodamine B, Butyl rhodamine B and Rhodamine 6G, respectively. There is a higher FRET efficiency between CdS and Neutral red with the value being 0.282. Moreover, coupling reaction between -NH_2 of Neutral red and -COOH of CdS -TGA leads to red shift of CdS fluorescence spectrum. The reaction also occurs between Safranine T and CdS. At the same time, the Fluorescence of ST is quenched by forming ST -CdS ground state complex. Chapter 6, CdS QDs modified by chitosan and polyethyleneimine can be quenched by Cu~(2+). According to the experimental result, we develop the novel sensor for Cu~(2+).

研究结果，吡啰红与CdS之间作用形式是染料聚集在CdS的表面使得CdS不易聚集，但同时也降低了CdS的发光的量子效率；罗丹明B、罗丹明6G、丁基罗丹明B均与CdS之间存在能量转移，位阻效应使得能量转移效率最高的BRhB的相对荧光强度增大最小；中性红与CdS的能量转移效率更高，同时推测可能使CdS-TGA上的-COOH与部分NR的-NH_2发生偶联导致粒子变大而发生荧光光谱的红移；番红花红与CdS是相互荧光猝灭，可能是ST的-NH_2与CdS-TGA上的-COOH发生偶联使得纳米粒子变大以及二者形成基态复合物的共同作用的影响。

Through this issue, we found that with 2% NaOH-NALC digested within 10 minutes in the rapid decomposition of the viscous sputum, in ensuring the effectiveness of digestion at the same time not too much because of the role of NaOH to kill bacteria (time to 20 minutes), and would not affect the role of magnetic particles; quantitative simulation of self-made through the application of clinical samples, we compared the acid-fast stain and auramine O staining of the detection limit, auramine O fluorescence staining against the backdrop of a clear dark green light acid-fast stain of Mycobacterium tuberculosis than the white background of the red eye-catching TB is more capacity, auramine O staining in the detection limit of 0.05 to 0.001 between Maxwell, higher than the acid-fast stain of Michael's 0.05 units, The former is more sensitive; In addition, the accumulation of magnetic nanoparticles of the samples after the smear, in the microscope show the number of TB was significantly higher than that of the specimens has not been enriched.

通过本次课题，我们发现用2%NaOH-NALC消化液可在10分钟内迅速裂解痰液的粘性介质，在保证消化效果的同时不会由于NaOH过强的作用杀死细菌（时间控制在20分钟内），亦不会影响磁性颗粒的作用；通过应用自制定量模拟临床标本，我们比较了抗酸染色和金胺O荧光染色的检出限，金胺O荧光染色黑暗背景下发出明显绿色亮光的结核杆菌较抗酸染色白色背景中的红色结核杆菌更容醒目，金胺O荧光染色的检出限在0.05~0.001个麦氏单位之间，高于抗酸染色的0.05个麦氏单位，前者敏感度较高；另外，经过磁性纳米颗粒富集作用后的标本涂片，在镜下显示其结核杆菌数量明显高于未经过富集的标本。

The methods used for collecting samples and establishing specific HAB cultures are described in detail. Methods of SEM and ESEM (environmental scanning electron microscope) for Gymnodinium were developed an...

建立了赤潮监测中样品采集处理、分离和纯化培养的技术体系；建立和优化了裸甲藻扫描电镜观察技术和环境样品的扫描电镜观察方法；提出了库尔特计数是相对简便、快捷、准确、可行的细胞计数方法；应用基于反相高效液相色谱的光合色素分析技术及原位荧光激发光谱测定方法，得出不同类群浮游植物对不同的营养盐限制存在不同响应机制的结论；用荧光强度来监测浮游植物生理状态及营养状态变化具有一定的可行性，HPLC和原位荧光两种方法指示浮游植物的生理状态存在一定差异。2。

Oil film thickness along the piston skirt in motored engine was measured, and the comparison with theory result was given.

最后在实验研究中，本文国内首次在活塞裙部油膜厚度中采用了激光荧光诱导的测试方法，主要工作是：分析了激光荧光诱导法的基本原理，建立了测膜厚的基本定量关系模型，通过实验分析了膜厚测试的各种影响因素，通过实验对不同香豆素荧光光谱和激发光谱特性进行了分析，通过实验确定了选取香豆素的种类和溶液浓度的基本原则，自主开发了以光纤为光学光路的测试系统并对系统进行了标定，对内燃机冷拖动状态的活塞裙部进行了实测，并与理论测试结果进行了对比。

XRD showed hexagonal wurtzite structure. Room-temperature PL spectra of perfectly overcoated CdSe/ZnS only showed a narrow band edge emission. Emission peak was great red-shifted relative to absorption and the shift increases as the size decreases. The size-dependence of shift could be explained very well in terms of excition fine-structure model and stress at interface owing to lattice mismatch contributes to the shift between CdSe and CdSe/ZnS with the same size.

结果显示表面修饰完善的样品的荧光光谱只有一个窄而强的带边发射；所有样品的荧光峰相对于1S-1S吸收峰有较大的红移，并且移动量随尺寸的减小而增加，利用激子的精细结构模型很好地解释了红移量和尺寸的关系；核／壳结构的样品的吸收峰和荧光峰相对于同尺寸的单量子点也有红移现象，这是由于CdSe和ZnS的晶格失配在界面上产生压力造成的。

The two-photon absorption cross-sections of these styryl derivatives were determined by Z-scan technique, and the benzothiazolium derivatives exhibited the largest TPA cross-sections (10〓s·cm〓/photon) and the best power limiting ability at high operable incident intensity. The results show that the benzothiazolium derivatives are promising organic compounds as two-photon absorption optical limiting materials. 2. Several organic dyes active to 800nm femtosecond laser with D-π-A structure were designed and synthesized.

研究了它们的上转换荧光、上转换激射和光限幅行为，通过改变染料分子共轭体系的大小，首次实现了在576nm-635nm范围内的双光子上转换激光波长的调节，其中噻唑啉碘盐类二苯乙烯化合物的双光子上转换激射峰（576nm）是迄今在1064nm皮秒激光作用下能够得到的最短波长的激光；阴离子显著改变上转换激射效率，但对上转换荧光峰和激射峰的位置影响很小；增加溶液的浓度，上转换荧光光谱和激射光谱红移，并在一定的浓度范围内，能够有效的提高上转换激射效率；利用Z-扫描技术测定了染料分子的双光子吸收截面，发现苯并噻唑类染料分子的双光子吸收截面高达10〓s·cm〓／photon，而且在较大的入射光强范围内，这类染料分子具有较大的光限幅能力，是一类作为双光子吸收光限幅材料很有潜力的染料分子。

The design was optimized for maximum signal intensity by simulation and experiments. The size of focal spot in the micro-channel capillary was optimized. Fluorescence signal was enhanced 2～3 times when increasing the diameter of focal spot from 10μm to 50μm (full of the inner wall of capillary), through which the sensitivity and signal-noise ratio were also improved. A technique of using refractive matching liquid was presented. The fluorescence signals were boosted up and the signal collection efficiency was also meliorated when using this method.

通过理论分析和实验优化本系统的设计：优化聚焦于微通道中的激发光斑大小，增大聚焦到毛细管内径中的激发光斑尺寸，使光斑尺寸由最小的10μm增大到50μm，光电倍增管接收到的荧光信号提高了2-3倍，从而提高系统的信噪比和灵敏度；提出在聚焦物镜处加折射率匹配液的方法，加匹配液后的荧光信号得到了增强，因而提高了荧光收集效率和灵敏度。

The fluorescent emission mechanism, and the some factors that affect the energy and the intensities of the fluorescent peaks were studied. The strong powder second harmonic generation efficiencies for complexes (1),(2),(3) and (4) were found. The determination of variable-temperature magnetic susceptibility for complex (3) and complex (5) was performed. The magnetic data were analyzed with their structure properties, and the further information about the magnetic coupling capacity of Carbamyldicyanomethanide as bridging ligand was obtained.

测试了配合物(1)，(2)，(3)，(4)的荧光光谱，从配体的结构特点等方面研究了该荧光光谱的产生机理，研究了影响荧光谱峰能量及强度的因素；发现了配合物(1)，(2)，(3)，(4)显示有强的二阶非线性倍频效应；测试了配合物(3)和(5)的变温磁化率，对所得磁数据进行了结构性质分析，进一步获得了甲氨酰二氰基甲基阴离子作为桥联配体的磁耦合信息。

The fluorescence spectra of Eu~(3+) may be used to study the structure ofcompounds.The band shift of Nd~(3+) in its absorption spectra,the red-to-orangeintensity ratio of the Eu~(3+) emission(~5D_0→~7F_2)/(~5D_0→~7F_1)and the yellow-to-blue intensity ratio of the Dy~(3+) emission~4F_(9/2→~6H_(13/2)/~4F(9/2→~6H(15/2)intheir fluorescence spectra may be used to study the degree of covalency of theLn-O bond and its relation with the electronegativity of M in Ln-O-M.

本文介绍了利用 Eu~(3+)的荧光光谱研究化合物的结构；利用 Nd~(3+)的吸收光谱的谱带位移和 Eu~(3+)的荧光光谱的红橙发射强度比(~5D_0→~7F_2)/(~5D_0→~7F_1)和 Dy~(3+)的黄兰比~4F_(9/2→~6H_(13/2)/~4F_(9/2→~6H_(15/2)，了解Ln—O—M中 Ln—O 化学键的共价程度以及 M 的电负性对共价程度的关系；利用吸收光谱、荧光光谱和磁化率研究稀土的价态以及利用磁化率研究化合物的组成。

Through this topic, we discover with the 2%NaOH-NALC digesting fluid may in 10 minute in rapid decomposition sputamentum resisting medium, during guarantee digestion effect not because the NaOH strong function will have killed the bacterium (timing control in 20 minutes), also will not affect the magnetic pellet the function; Through the application self-restraint quota simulation clinical specimen, we have compared the acid-fast stain and the pyoktanin yellow O fluorescence dye pick out limit, the pyoktanin yellow O fluorescence dyes the dark background to after-crop the obvious green bright the bacillus tuberculosis to compare in the acid-fast stain white background the red bacillus tuberculosis to accommodate strikingly, the pyoktanin yellow O fluorescence dyes picks out limits between 0.05~0.001 Mai unit, is higher than the acid-fast stain 0.05 Mai unit, the former sensitivity is high; Moreover, concentrates the function after magnetic nano particles the specimen smear, under the mirror demonstrated that its bacillus tuberculosis quantity is higher than the specimen which obviously has not passed through concentrates.

通过本次课题，我们发现用2%NaOH-NALC消化液可在10分钟内迅速裂解痰液的粘性介质，在保证消化效果的同时不会由于NaOH过强的作用杀死细菌（时间控制在20分钟内），亦不会影响磁性颗粒的作用；通过应用自制定量模拟临床标本，我们比较了抗酸染色和金胺O荧光染色的检出限，金胺O荧光染色黑暗背景下发出明显绿色亮光的结核杆菌较抗酸染色白色背景中的红色结核杆菌更容醒目，金胺O荧光染色的检出限在0.05~0.001个麦氏单位之间，高于抗酸染色的0.05个麦氏单位，前者敏感度较高；另外，经过磁性纳米颗粒富集作用后的标本涂片，在镜下显示其结核杆菌数量明显高于未经过富集的标本。

But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊！不用提了。提到肉，真是糟透了。