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The reducing product is monoamine and diamine with 72 % yield. Based on the characteristic of mo- noamine with strong fluorescence, this paper has analyzed the content of mo- noamine and diamine by diazotization method combined...

根据单胺具有强烈荧光的特点,采用重氮化法和荧光分光光度法相结合,测出混合胺中的单胺与双胺合量分别为22.54%和67.22%,其余是无机盐。

By using tricks from optics and physiology, they were able to watch the steps of viral assembly, budding, and even scission off the cell surface.

研究人员在蛋白分子上涂上荧光,放置在细胞内紧贴细胞内壁的位置,当分子聚集在一起时,荧光的色彩会发生变化。

And mices'embryos of various ages were gotten and 81 of 793 embryos had fluorescence. Secondly, we directly injected the gene/ liposome complex into 5 male mice's spermaduct and 75 of 810 embryos had fluorescence.

其中睾丸注射法、输精管注射法和外源基因/精子共孵育法分别各处理公鼠5只,7d后各配母鼠25只,结果分别冲出胚胎793枚、810枚和357枚,荧光显微镜下检测到荧光的分别有81枚胚胎(10.2%)、75枚胚胎(9.26%)和53枚胚胎(14.9%)。

The onset of EGFP fluorescence and its distribution in the Wistar rat corneas were determined through fluorescence stereoscope.

建立大鼠异体角膜移植模型,用含rAAV1-EGFP转染液的培养基在37℃孵育SD大鼠角膜植片同时浸泡缝线18h,再用此缝线间断缝合,将植片移植到大鼠角膜植床,术后通过荧光体视镜观察Wistar大鼠角膜出现EGFP荧光的起始时间及分布情况。

Methods A lamellar flap was produced in the rabbit cornea with a microkeratome. The flap interface in whole corneas and corneal beds were soaked in the rAAV –EGFP solution for 10 min, BSS solution was applied in the control group. The corneas were harvested at day 1, 3, 14, and 21 after application for the structure analysis of cornea by HE staining. Expression of the transferred gene was monitored by fluorescence stereoscope and laser confocal microscope. Results After 3d of infection with rAAV-EGFP ,slight fluorescence was detected in rabbit cornea by fluorescence stereoscope .

微型角膜刀制作兔角膜基质瓣;转染组用含rAAV-EGFP转染液浸泡角膜瓣和角膜基质床10 min,对照组用等量BSS液浸泡角膜瓣和角膜基质床10 min,不同时间点(1、3、7、14、21d)通过荧光体视镜观察角膜出现EGFP荧光的起始时间及分布情况,收集角膜,其中一半用激光共聚焦显微镜观察、照相,另一半固定后行HE染色。

It would be helpful to understand the bioluminescent essence and origin of the firefly and other bioluminescence organism.

这将有助于理解萤火虫及其它荧光生物自发荧光的本质和起源进化过程。

A compound, which had isoindole unit in its molecule with strong fluorescence intensity, would form when the polymer particles were reacted with primary amine.

该聚合物颗粒本身的荧光很弱,但可与伯胺作用,形成具有强荧光的异吲哚类化合物。

In the latter case the graft and host tissue mixed togethor. In the grafts there were yellow-green fluorescence-bearing catecholaminergic neurons and TH immunoreactive neurons appearing as fusiform or polyangular shape.

荧光显微镜下可见移植物内有发黄绿色荧光的儿茶酚胺能神经细胞,免疫细胞化学显示移植区内有TH阳性神经细胞,后者为梭形或多角形。

Therefore, fluorescein isothiocyanate conjugated lectins, namely concanavalin agglutinin, wheat germ agglutinin and soybean agglutinin, were used to localize lectin receptors on plasma membrane of male and female gametes and other relevant sexual cells, as well as embryos of various developmental stages of Nicotiana tabacum and Torenia fournieri by cooled charge coupled device and Confocal Laser Scanning Microscope.

从而证实:在本实验中用的几种酶组合对凝集素受体的影响并不大,即由酶解及解剖得到的雌性细胞的荧光在分布形式及强度上没有明显的差异;对比原位酶解与离体酶解得到的雌性细胞的荧光,证实机械操作对荧光的分布形式及强度也没有明显的影响。

Based on the changes in the spectrum characters of guest molecules producedthrough inclusion complex formation with cyclodextrins, we have carried out two aspects of investigation:First, the mechanisms for the host-guest recognition reactions have been studied, such as the interactions between 3-CD and curcumin, the main constituent of the rhizomes of the plant Curcuma longa, the recognition of p-CD towards rubidate, the effective component of the Chinese traditional medicine madder, and the reactions of p-CD towards the antileprotic drug dapsone and the antiinflammatory drug nabumetone: The formation of supramolecular complex between P-CD and guest molecule has been discussed and verified in virtue of multitudinous means such asultraviolet-visible absorption, fluorescence emission, infrared spectrogram and so on. The influence of addition of linear alcohols on the supramolecular system has been studied and the reason why the noncovalent interactions in the three-component system could result in a decrease in the apparent association constant of the inclusion complex has been revealed.

本论文基于环糊精对被包结客体光谱性质的改变,开展了两大方面的研究:β-环糊精对姜黄属植物根茎主要成分—姜黄素、中药茜草有效成分—茜草双酯、抗麻风药物氨苯砜及抗炎药物萘丁美酮的主-客体识别反应机制研究:借助超分子包合物的紫外吸收光谱、荧光光谱或红外吸收光谱等手段,通过多种方法探讨并验证了超分子包合物的形成;研究了直链醇的加入对超分子体系的影响,揭示了三元体系中非共价相互作用导致超分子包合物表观结合常数降低的原因:对超分子包结反应进行了热动力学研究,探讨了各热动力学参数的变化趋势并从熵焓互补的角度考察了超分子包结过程;通过荧光猝灭实验进一步揭示了水相中β-环糊精增敏客体荧光的本质原因,从而为超分子化学提供新的理论和研究方法。

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The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应,而且减少跟风的可能性。

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