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The fluorescence properties of complexes of europium with β-diketone and terbium with benzoic acid and its derivatives were analyzed and the synergic effect of the second ligand was illustrated from the energy matching of the minimum triplet state of ligands and the resonance energy level of rare earth ions.

提出了掺杂离子荧光增强效应的机理。 4。首次采用ab initio CIS方法,在劈裂价层基组3—21G水平上对各配体进行了量化计算,系统分析了前线分子轨道特征和激发态的能级,从配体最低激发三重态能级与稀土离子共振能级的能级匹配,分析了铕与β-二酮的配合物的荧光性能和铽与苯甲酸系列衍生物的配合物的荧光性能,解释了第二配体的协同效应。

an approach divides the sonoluminescence from the sound field by using test tube with black plastics and the isotropic light trap is applied to collect sonoluminescence in the trough which irradiates hematoporphyrin out of the sound field.the results of our work suggest that ultrasonically induced cavitation produces nonequilibrium high temperature, emits shock waves, together with the cavitation energy in hematoporphyrin solution activating hematoporphyrin.

从超声空化产生的声致荧光出发,采用试管外包裹黑色塑料的方法将大量生理盐水的声致发光与其余能量形式分开,在相同条件下,使用集光球收集水槽中盐水的声致荧光,将荧光引出声场单独作用血卟啉。结果表明,水槽中盐水的空化热能、冲击波与试管中血卟啉溶液空化产生的高温、高压、冲击波等多种能量的合作用激活血卟啉。

Firstly, the pGL3-Control vector was reconstructed , the pGL3-Enhancer vector deletious of SV40 promoter gene was obtained . Then the IFN-β promoter gene was cloned into the pGL3-Enhancer vector and pGL-IP21, the Luciferase reporter plasmid with IFN-β promoter was OK. The availability of pGL-IP21 was verified by NDV ,the inductor of IFN-β, the Luciferase activity was assayed in cells transfected with pGL-IP21 by Luminometer.

首先将pGL3-Control载体进行了改构,除去了SV40启动子基因,获得了pGL3-Enhancer载体,将获得的IFN-β启动子基因连入载体中,构建了带有人IFN-β启动子基因的荧光素酶报告质粒IP-21,并且通过实验证明所构建的质粒在干扰素的诱导剂NDV的作用下能够表达荧光素酶活性,照度计检测荧光素酶活性增强,从而验证了所构建的重组质粒的有效性。

Then we transfected transitorily the recombinant of green fluorescent protein gene and middle molecular weight neurofilament cDNA into wide type N2a (N2a/wt) and N2a/tau40 to observe the effect of tau accumulation on GFP-NFM fusion protein transport in cellular processes in living cells. At last we used an apoptotic inducer, camptothecin (an inhibitor of topoisomerase-1) to treat N2a/wt and N2a/tau40 cell lines, and compared their apoptotic response.

主要结果如下:一、tau蛋白过度表达和聚积对细胞形态的影响:倒置显微镜下观察两种细胞的形态,发现N2a/wt细胞的突起多而长,而N2a/tau40细胞胞体变圆,突起明显缩短;免疫印迹结果显示转染了tau40的细胞内tau的免疫反应约增加14倍,免疫荧光结果显示N2a/tau40细胞胞体内呈现出较强的红色荧光,tau主要分布在核周和突起起始部分的胞质内,而N2a/wt细胞内的荧光很弱。

From the photophysical behaviors of 1, 3, 4-triaryl-2pyrazoline compound (TPP16) at low temperature, it can be considered that the twisted configuration between pyrazoline ring and the non-substituted phenyl group on the position 1 is the fluorescence configuration of conjugated system Ⅰ, and the twisted configuration between pyrazoline ring and P-nitrobenzene group is fluorescence configuration of conjugated system Ⅱ.

研究了1,3,4-三芳基-2-吡唑啉化合物TPP16在低温时的发光情况,发现:在低温77K下,共轭体系Ⅰ(苯基-N1-N2=C3-苯基)在非极性溶剂中不发荧光,在极性溶剂中有较强荧光发射;而共轭体系Ⅱ(N1-N2=C3-C4=对硝基苄叉)在非极性溶剂或极性溶剂中均有荧光发射,表明N1所联取代基与吡唑啉环相扭曲的构象是共轭体系Ⅰ的发光构象,而对硝基苄叉与吡唑啉环相扭曲的构象是共轭体系Ⅱ的发光构象。

A group of pyrylium salts with different substituents has been synthesized in this work. Their fluorescence life time, quantum yield and quenching constant by BVE have also been measured. It was found that a strong interaction was observed between BVE and pyrylium salt which contains an electron-withdrawing group, and contrary, the introduction of electron-repulsive groups to the pyrylium salt will decrease this effect. It indicated that these reactions possess the characteristic of electron transfer.

本工作合成了一组带不同取代基的氧鎓盐化合物,并对它们的荧光寿命、荧光量子产率以及用丁基乙烯基醚进行猝灭的荧光猝灭常数进行了测定,发现带拉电子基的氧鎓盐化合物和BVE间存在着较强的相互作用,相反,推电子基的引入则减弱了这种影响,表明反应具有电子转移性质。

Therefore, zinc oxide and Eu~(2+)-activated strontium aluminate green phosphors were synthesized for the fluorescence conversion of near ultraviolet light-emitting diode, and their properties were examined by means of photoluminescent spectra, thermoluminescent spectrum, X-ray photoelectron spectroscopy and X-ray diffraction.

本研究针对近紫外LED荧光转换材料的要求,采用荧光光谱,热释光谱,X射线光电子能谱,X射线衍射等分析手段,重点研究ZnO绿色荧光粉和Eu~(2+)激活铝酸锶绿色长余辉荧光材料的合成,或提高发光效率和稳定性的新方法及其相关机制。并在以下几方面取得了一些进展: [1]将ZnS与一定量的卤化物MX(X=F,Cl,Br,I;M=Na,K,NH_4,Zn,ZrO)一起煅烧合成出发光性能优良的、具有六方纤维锌矿结构的ZnO绿色荧光粉。

Three kinds of fluorescence polymer with the structure of acridine yellow were synthesized and the sensor for measuring nucleic acid was also fabricated.

论文第三部分开展了以荧光高分子为敏感试剂的新型光化学传感器载体研究,合成了N,N-二甲氨基对苯甲醛缩醛类聚合物,考察了苯环上不同取代基对荧光性能的影响,研究了该类敏感膜对乳酸、尿嘧啶、胸腺嘧啶和腺嘌呤的响应性能;合成了三种具有吖啶黄结构的荧光高分子,制成了测定核酸的传感器。

The residual stress can also change the transition energy of excitons.

薄膜应力是影响ZnO薄膜紫外荧光发射性能的重要因素,较小的残余应力对ZnO薄膜的紫外荧光发射是有利的,残余应力的存在可以改变ZnO薄膜紫外荧光发射能量。

Assays of AKT and MPF activities AKT or MPF kinase activity was measured by histone H2B kinase or histone H1 kinase assay.The frozen eggs were treated with the same method as we previously reported.The radioactivity on the filter paper was counted with a BECKMAN scintillation counter.

免疫荧光分析的结果显示,结合Ser473位磷酸化AKT的绿色荧光FITC在GV期卵母细胞中均匀分布在胞浆中,而在S期的受精卵中绿色荧光分布于细胞膜,AKT的膜定位表明在S期的受精卵中它可能处于活化状态。

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