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The reflective law of color fluorescent ink half tone is generalized by utilizing the idea that the reflected light by fluorescent print is divided into two parts: primary streams which consist of originally incident light and fluorescent streams which are created by absorption of the primary stream. And an exponential matrix modelizing the layer of fluorescent ink is utilized to compute the energy of fluorescence.

把荧光半色调印刷品反射出来的光分成两个独立的部分,即由最初入射光组成的主光流和由于吸收了主光流而产生的荧光流;并且采用了一个指数矩阵来描述墨层产生的荧光光强,从而得出了荧光半色调图像色彩的光反射规律。

There six chapters in this dissertation: the overview on the study of probing for reactive oxygen species, spin-labelled fluorophore as probe for free radicals, Fe -EDTA chelate-induced aromatic hydroxylation of terephthalate as a new method for the evaluation of hydroxyl radical-scavenging ability, study of fluorimetric method for the determination of peroxynitrite, study of spiro-formed rhodamine B hydrazide as a novel fluorescent probe in analytical chemistry, the conclusion and prospect of the present research work.

本论文分六章,包括生物活性氧表征的研究进展、自旋标记荧光探针表征自由基的研究、利用Fe-EDTA引发对苯二甲酸芳环羟基化反应评价抗氧化剂清除羟基自由基能力的研究、荧光法表征过氧亚硝酸根的研究、螺环型罗丹明B酰肼化合物作为新型荧光探针的性质及应用的研究、结论与展望。

The Molar conductivity indicates that all the complexes are nonelectrolyte. IR spectra show the ligand is bonded with RE ion through its oxygen atom in hydroxyl group of phenol; NO3 is coordinated as a symmetrical chelating bidentate group; the coordinated atoms of DMF and DMSO are the oxygen atoms in carbonyl and sulfoxyl groups. Fluorescence spectrum suggests that the Tb3+complexes have characteristic luminescence and its fluorescence intensity is enhanced after doping with La3+, Gd3+, Y3+, influence of doping ions and neutral ligands have also been discussed.

摩尔电导率表明,所有配合物均为非电解质;红外光谱及核磁共振氢谱表明,对叔丁基杯[8]芳烃通过酚羟基的氧原子与稀土离子配位;硝酸根为双齿螯合配位;DMF和DMSO则分别通过羰基氧和亚砜基氧与稀土离子配位;配合物的荧光光谱表明,所有Tb的配合物具有较强的特征荧光,掺杂La~(3+)、Gd~(3+)、Y~(3+)离子对Tb~(3+)的荧光有较强的增强作用。

The result shows: at different temperatures the outcomings obtained in determining dissociation constants with such two theories can be seen effective, but the doubly reciprocal curves of quenching equation are not conformed to quenching curves based on dynamic and static quenching mechanism as those of fluorescence enhancement.

通过荧光和紫外方法研究盐酸左氧氟沙星、氟罗沙星、加替沙星和沃氟沙星四种喹诺酮类药物与人血清白蛋白的作用,在不同温度下对荧光加强和猝灭理论公式的等价性和精细差异进行了比较,结果表明:在不同温度下两种理论公式尽管在求取解离常数上所得结果都可视为有效,但猝灭方程的双倒数图反而不及荧光加强方程的双倒数图与体现动态、静态猝灭机理的猝灭曲线一致。

Secondly, we provide a two-level model to depict the one-, two-and three-photon fluorescent process and with it to calculate the one-, two-and three-photon fluorescence intensity dependence of the incident excitation intensity and time. We study the fluorescence intensity when excitation light's time distribution are square pulse、Gaussian pulse and sech pulse. We find that the influence of excitation light's time distribution on the two- and three- photon fluorescence peak intensity is larger than the one-photon fluorescence peak intensity. Two-level model can account for the behavior of saturation fluorescence.

然后用二能级模型解释了单、双、三光子激发原理,在理论上推导出了单、双、三光子吸收诱导荧光强度与脉冲强度以及脉冲作用时间的关系,并进一步研究了入射光时间分布分别为矩形、高斯型、双曲正割型时激发溶解在氯仿溶液中的C22H22N4O样品所产生的荧光峰值光强,结果表明激发光的时间分布对双、三光子荧光峰值强度的影响比对单光子的影响要大。

Since the molecular clip of glycoluril is also an important supramolecular host but few studies have been done in the field of fluorescent chemosensor,we proceeded with some experiments and research in this field as a trial and designed a series of novel fluorescent chemsensors including different three-dimensional cavities by introducing the fluorophores into the receptor.Their recognition and sensing abilities toward various metal ions and neutral guests were studied.

因此,我们(来源:A1dBC论文网www.abclunwen.com)做了一些尝试性的研究,在传统的甘脲主体化合物上引入荧光发色团,设计了一系列新型的、拥有不同立体空腔的荧光分子夹,并研究了它们对各种金属阳离子以及酚类中性客体分子的识别和荧光传感性能。

PartⅡThe mechanism elucidation about effects ofα-(2,3)/(2,6)sialic acid on the Cx43gap-junction functions.(1) Westernblotting experiment showed that the decrease of sialic acid didn\'t changethe Cx43 expression and its phospholation level.(2) Westernblotting experiment showed sialidase didn,t change the ZO-1 expression,IP and confocal experiment showed sialidase improved the interaction of Cx43 andZO-1.(3) Westernblotting experiment showed sialidase didn\'t change N-cadherin expression,IP and confocal experiment showed sialidase promoted the complex formation ofCx43 and N-Cadherin.(4)Sialidase could increase the ERK1/2 phospholation level,and enchanedintercellular homotypic adhesion,Immunofluorometric assay showed sialidase couldpromote the N-cadherin cluster on the membrance.

第二部分:α—(2,3)/(2,6)唾液酸对肿瘤细胞CX43间隙连接功能影响的机制研究1、Westernblotting结果表明降低细胞膜表面唾液酸并不改变Cx43的表达及其磷酸化水平:但Cx43连接斑形成增多;2、Westernblotting结果表明唾液酸酶作用后细胞ZO—1表达没有改变,IP及免疫荧光共定位结果表明唾液酸酶作用后促进了Cx43与ZO-1的结合;3、Westernblotting结果表明唾液酸酶作用后细胞N-cadherin表达没有改变,IP及免疫荧光共定位结果表明唾液酸酶作用后促进了Cx43与N-Cadherin复合物的形成;4、唾液酸酶作用后细胞内ERK1/2磷酸化水平明显增加,细胞间同质粘附增加,以及免疫荧光表明唾液酸降低后可促进N-cadherin的膜成簇。

We propose a new mechanism of ASFC, which based on energy transfer among the centers within the inhomogeneously broadened spectral profile. We discuss it in two situations: small energy mismatch and large energy mismatch. The dependence of relative cooling efficiency on excitation photon energy and temperature is also discussed in evaluating the new mechanism.

提出了荧光光谱的非均匀线形内不同发光中心的能量传递产生荧光制冷效应的理论,分别对小能量失配的传递过程和大能量失配的传递过程进行了研究,认为在一定的条件下这两种过程都可以产生荧光制冷效应,并给出了相对制冷效率。

Predicted Model of Aggregation of Molecules in Chinese Herbal Drugs by Support Vector Machines;2. The aggregation behavior of phenylhydrazone and hemicyanine,in Z-type LB multilayers has been studied by absorption, steady state and time-resolved fluorescence spectroscopies.

利用吸收、稳态和时间分辨荧光方法研究了苯腙和半花菁分子在Z型LB多层膜中的聚集体性质,苯腙和半花菁吸收光谱的红移和兰移,说明它们分别以J和H型聚集体形式存在于LB腹中苯腙和半花菁LB膜的荧光发射带分别在560nm和584nm处,亦分别对于它们溶液的荧光谱显示出红移和兰移的特征。

In order to get purified purified commercially luciferase, we selected whole gene fragment of Photinus pyralis as well as gene fragment deleting 18-nucleotide at 5'termination, which was synthesized according to P〓 template.

为了能得到商品纯的虫荧光素酶,将取自P〓质粒中的全长的北美萤火虫荧光素酶基因片断,及以P〓为模板合成的5'端缺失18个核苷酸的虫荧光素酶基因片断。

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