荧光的

Tetraploid embryos could be produced by electrofusion at the stage of two-cell embryos, which could develop to blastocysts followed by fusion of cytoplasm and nucleus and cleavage in vitro. During the fusion of cytoplasm, the DNA methylation levels of the fused embryos are as high as these of two-cell diploid embryos in vivo Then the embryos are rapidly demethylated when the nucleus begin to fuse, resulting in the lowest DNA methylation levels when the nucleus are fused completely. After that, the DNA methylation levels of the fused embryos are gradually increased until the morula stage. However, whereas an asymmetric distribution of DNA methylation is established in vivo-derived blastocysts with a higher methylation level in the inner cell mass than that in the trophectoderm, we can not detect the asymmetric distribution in most in vitro-derived tetraploid blastocysts.

结果表明：利用电融合方法制备的小鼠四倍体胚胎在体外培养体系中经历细胞质融合、细胞核融合及细胞继续分裂发育直到囊胚期的过程，在细胞质融合的时候胚胎卵裂球同体内体外培养二倍体胚胎一样，呈现高度甲基化状态；在细胞核开始融合的时候，甲基化水平急速下降，在细胞核完全融合的时候甲基化水平达到最低点；随着胚胎继续分裂，胚胎甲基化水平逐渐增加，在桑葚胚期甲基化水平最高；但是囊胚期四倍体胚胎内细胞团同滋养层细胞甲基化荧光信号没有差别，这与体内体外培养二倍体囊胚内细胞团细胞甲基化荧光强度高于滋养层细胞甲基化荧光强度不同。

Tetraploid embryos could be produced by electrofusion at the stage of two-cell embryos, which could develop to blastocysts followed by fusion of cyto-plasm and nucleus and cleavage in vitro. During the fusion of cytoplasm, the DNA methylation levels of the fused embryos are as high as these of two-cell diploid embryos in vivo Then the embryos are rapidly demethylated when the nucleus begin to fuse, resulting in the lowest DNA methylation levels when the nucleus are fused completely. After that, the DNA methy-lation levels of the fused embryos are gradually increased until the morula stage. However, whereas an asymmetric distribu-tion of DNA methylation is established in vivo-derived blastocysts with a higher methylation level in the inner cell mass than that in the trophectoderm, we can not detect the asymmetric distribution in most in vitro-derived tetraploid blastocysts.

结果表明：利用电融合方法制备的小鼠四倍体胚胎在体外培养体系中经历细胞质融合、细胞核融合及细胞继续分裂发育直到囊胚期的过程，在细胞质融合的时候胚胎卵裂球同体内体外培养二倍体胚胎一样，呈现高度甲基化状态；在细胞核开始融合的时候，甲基化水平急速下降，在细胞核完全融合的时候甲基化水平达到最低点；随着胚胎继续分裂，胚胎甲基化水平逐渐增加，在桑葚胚期甲基化水平最高；但是囊胚期四倍体胚胎内细胞团同滋养层细胞甲基化荧光信号没有差别，这与体内体外培养二倍体囊胚内细胞团细胞甲基化荧光强度高于滋养层细胞甲基化荧光强度不同。

Tetraploid embryos could be produced by electrofusion at the stage of two-cell embryos, which could develop to blastcysts fellowed by fusion of cytoplasm and nucleus and cleavage in vitro.After fusion of cytoplasm, the DNA methylation levels of the fused embryos was very high as well as two-cell diploid embryos in vivo.Then the embryos was rapiddly demethylated when the nucleus begin to fuse, resulting the lowest DNA methylation levels when the nucleus fused completely.After that, the DNA methylation levels of fused embryos were gradually increased until the blastocysts stage.However, whereas an asymmetric distribution of DNA methylation was established in an vivo-derived blastocysts with a higher methylation level in the inner cell mass than in the trophectoderm, in most vitro-derived tetraploid blastocysts, we can not detect the asymmetric distribution.

结果表明：利用电融合方法制备的小鼠四倍体胚胎在体外培养体系中经历细胞质融合、细胞核融合及细胞继续分裂发育直到囊胚期的过程，在细胞质融合的时候胚胎卵裂球同体内体外培养二倍体胚胎一样，呈现高度甲基化状态；在细胞核开始融合的时候，甲基化水平急速下降，在细胞核完全融合的时候甲基化水平达到最低点；随着胚胎继续分裂，胚胎甲基化水平逐渐增加，在囊胚期甲基化水平最高；但是囊胚期四倍体胚胎内细胞团同滋养层细胞甲基化荧光信号没有差别，这与体内体外培养二倍体囊胚内细胞团细胞甲基化荧光强度高于滋养层细胞甲基化荧光强度不同。

We observed that a majority of dsRNA was localized in the nuclear periphery discontinuously after 24 hours of transfection. Time course showed that the labeled dsRNAs were maintained in cultured silkworm cells for up to 5 days. After six days, the signal was too weak to be detected.

用荧光显微镜观察FAM标记的dsRNA在细胞内的定位，发现在转染24小时后，dsRNA开始越来越多的聚集在细胞核的周围，并呈不连续分布，细胞内的荧光强度也越来越强。dsRNA在家蚕细胞中可以维持5天的时间，但到第6天时细胞内已检测不出荧光信号。

Based on the different permeability of DNA-intercalant dyes YO-PRO-1 and propidium iodide to the membrane of viable, apoptotic and necrotic cells, cell samples were stained with 4μmol/L YP and 4μg/ml PI for 10 min, and the fluorescence intensity of both YP and PI were measured by fluorometer at Ex/Em wavelength of 485/538nm and 530/590nm, respectively.

根据正常细胞、凋亡细胞和坏死细胞的细胞膜对核酸荧光染料的不同选择通透性，用4μmol/LYO-PRO-1和4μg/ml碘化丙啶(Propidiumiodide，PI)染色96孔板中的细胞样品。分别在485/538和530/590的检测波长下借助荧光分光光度计检测细胞样品孔的YP和PI荧光强度。

The effects of excitation light source , grating of spectrometer and emis sion detector et al on the fluorescent spectra are studied.

介绍了荧光光谱测量系统的测量原理，讨论了激发光源、单色仪光栅、样品的制备、激发光的激发位置和入射角、探测器、荧光光谱波段等测量条件的选取对激光玻璃荧光测量结果的影响。

Comparably, we have synthesized three conjugated dendrimers bearing triphenylamine moiety as dendrons and benzene as core, which possess ACQ properties.

考察了以蒽为核的dendrimers在低温和室温下的荧光光谱，发现在低温时，它们的荧光强度显著增强，这是由于在低温时，分子内的振转运动受到限制，导致了荧光增强。

The immunofluorescence was developed with the mouse anti-VP4 serum. The results indicated that there was green-yellow fluorescence on the cell surface of pNZ8112-VP4 and pNZ8112-VP4-LTB when induced. The cells uninduced did not display an immunofluorescence reaction and were dyed red by Evans blue.

采用抗VP4的鼠血清进行免疫荧光试验的结果显示，pNZ8112-VP4和pNZ8112-VP4-LTB诱导后的菌体表面能看到黄绿色荧光，而诱导前的菌体则没有荧光反应，且细胞被伊文思蓝染成红色。

In order to develop new DNA probes,we have synthesized two kinds of probes bipyrene ratiometric fluorescence probes and long wavelength Ruthenium polydiimine complex,the following work have been carried out:Fluorescent DNA probes with 1,6-hexanediyl as the linker between two pyrenes, phenylpyrenes or phenylethynyl pyrene fluorophores were synthesized(Py-1,Py-2 and Py-3) and their interactions with DNA were studied by UV-Vis absorption spectra,fluorescence spectra and viscosity measurements.

在前人的基础上，为了更好的识别DNA，本论文设计合成了两类DNA探针——双芘类比率型DNA荧光探针和长波长钌的配合物，具体如下：以己二胺为柔性链，以芘及其衍生物为发色团合成了探针Pyl-3，并通过荧光光谱，荧光紫外吸收光谱和黏度测试研究了探针与DNA的结合方式。

The development of low-tension fluorescence materials of Field Emission Display and nanometer zinc oxide have been briefly summarized in the paper . Zinc oxide was thought to be a good low-tension fluorescence materials .

本文简单总结了和场发射低压荧光材料研究现状和纳米氧化锌的研究进展，认为氧化锌是很有前途的一种低压荧光材料，但其作为低压荧光材料还需要很多的改进，最主要是粉体的表面形貌和发光效率及发光色纯度等。

But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊！不用提了。提到肉，真是糟透了。