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The results showed that the DF excitation spectrum was obviously similar to the photosynthesis action spectrum; the DF signal changes with the intensity of excitation light source variation behaved as 'photoinhibition' phenomena of photosynthesis; and the DF intensity has an excellent correlation with photosynthesis rate at room temperature.

实验结果表明:延迟荧光激发谱与光合作用谱存在着明显的相似,延迟荧光强度随着激发光源光强的变化表现出类似的光抑制现象,以及在室温条件下延迟荧光强度与光合速率有着很好的相关性。

The results show that very bright and photostable Cy3 doped core-shell silica fluorescent nanoparticles...

结果表明,分别采用人免疫球蛋白或多聚赖氨酸修饰的Cy3为内核材料,都能制备荧光强度高、荧光稳定性强和染料泄漏极少的Cy3嵌入的核壳荧光纳米颗粒。

The study on the photoinhibiton of Pistachio shows: the exposure of Pistacia vera seedlings to high irradiance induced lowering of apparent quantum yield , maximal fluorescence , minimal fluorescence , variable fluorescence , photochemical efficiency of PS Ⅱ, and photochemical quenching , but raising the Non-photochemical quenching , this indicates that high irradiance reduces the quantities of oxidized Q〓, which depresses the ability of electron transport from Q〓 to Q〓, and lowers the electron transport rate and the photosynthesis rate.

对&光抑制&研究表明,强光照射后,叶片表观量子效率、最大荧光、初始荧光、可变荧光、光化学效率、和光化学猝灭系数下降,而qN升高,说明强光处理降低了Q〓的氧化态数量,使Q〓→Q〓传递电子的能力下降,进而导致了电子传递速率和光合速率的下降。

A pair of FRET(frequency resonance energy transfer) donor-acceptor chromophores were simultaneously labeled on immunoglobulin G at varied ratios to prepare the spectroscopically encoded core materials,which were then housed inside a silica shell by the hydrolysis and polycondensation of tetraethoxysilane in water-in-oil microemulsion to synthesize the optically encoded silica nanomaterials.

采用两种FRET供受体荧光染料按不同的比例与载体材料人免疫球蛋白作用制备荧光编码的载体材料,以荧光编码的载体材料为内核材料,利用氨水催化硅烷化试剂在反相微乳液中水解包裹内核材料制备成一系列多色光学编码硅壳纳米材料。

That of partial dissociated phycobilisomes indicated three bands: F652, F660 and F685, characteristic of C-phycocyanin, allophycocyainn and torminal emitters respectively.

部分解离藻胆体的荧光光谱的主峰位移至652nm:次峰位于685nm;660nm为一弱荧光发射肩。它们依次为C-藻蓝蛋白,末端发射体和别藻蓝蛋白的荧光。

Sound enamel surfaces were dematerialized in bacteria model in vitro. QLF was used to analyze the area of lesions, the loss of fluorescence and ΔQ on enamel surfaces after demineralization. Confocal laser scanning microscopy was used to quantify the mineral content after demineralization.

将30个牙釉质块开窗后分为3组,分别使用氟含量1 450 mg/L的氟化钠牙膏、单氟磷酸钠牙膏和无氟牙膏的处理液处理,并在体外恒化器龋病模型中进行脱矿循环,定量光导荧光技术分析病损脱矿后的面积、荧光损失和总荧光损失量,激光扫描共聚焦显微镜分析脱矿后矿物质含量变化。

Based on studies on the characteristics of ultraviolet-visible absorption spectra, ESR and ~(13)C NMR spectra, the three dimensional fluorescence spectra of natural and treated ambers with fluorescence are studied by using the three dimensional fluorescence spectrophotometer.

基于紫外可见吸收光谱、ESR和13C NMR谱的研究基础,以三维荧光分光光度计为主要测试分析方法,对具有荧光性质的天然与处理琥珀的三维荧光光谱进行了剖析。

When stained with TRICT-conjugated phalloidin, both the nuclei and nuclear matrix specimens were found to give off specific, red fluorescent signals which represent the location of F-actin, and the pattern of TRITC signals distribution in the nuclei and nuclear matrix showed similarity with that of FITC signals.

在荧光显微镜下观察到:代表肌动蛋白的特意性荧光分布在细胞核和细胞骨架中,说明肌动蛋白是细胞核和核骨架的固有成分;代表F-肌动蛋白的特异性荧光存在于细胞核和核骨架中,说明细胞核和核骨架含有F-肌动蛋白。

Optimal conditions for labeling MFs were tested. Best images were obtained with low fluorescent phalloidin concentration and shorter fixation time. 10nmol/L fluorescent phalloidin was found to be the optimal concentration for MFs localization in BY-2 cells.The distribution of MTs and MFs array in tobacco BY-2 suspension cells during the cell cycle were investigated with confocal microscopy.

采用间接免疫荧光定位及荧光定位技术结合激光共聚焦扫描显微镜等技术,对烟草BY-2悬浮细胞周期中微丝标记的最佳条件、微管列阵与微丝列阵的分布状况进行了研究,结果表明:10nmol/L荧光鬼笔环肽是活体细胞标记微丝的最佳浓度。

CoCl2 (a chemical hypoxia-mimetic agent) was used to establish the chemical hypoxia-induced PC12 cell injuries model. Sodium hydrosulfide was used as a H2S donor. The viability of PC12 cells was measured by CCK-8 assay. The percentage of apoptotic cells was assessed by propidium iodide stain flow cytometry. The morphological change of apoptotic cells was tested by using the chromatin dye Hoechst 33258. The mitochondrial membrane potential was analyzed by rhodamine 123 staining and photofluorography. The level of reactive oxygen species in PC12 cells was measured by DCFH-DA staining and photofluorography.

应用化学性缺氧模拟剂CoCl2在PC12细胞建立化学性缺氧损伤模型;以硫氢化钠作为H2S的供体;应用CCK-8比色法检测细胞存活率;碘化丙啶染色流式细胞技术检测细胞凋亡率;Hoechst33258染色检测细胞凋亡的形态学变化;罗丹明123(Rh123)染色荧光显微镜照相检测细胞线粒体膜电位;2',7'-二氯荧光黄双乙酸盐染色荧光显微镜照相检测细胞内的活性氧水平。

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The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

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