荧光的

The basic structure of chromogen in PFBs remains the same, so PFBs have fluorescent properties of traditional FBs. Covalent bonds between chromogen and macromolecule chains enhance their photochemical stability, increase their whitening performance and fluorescence quantum yield.

聚合型荧光增白剂中基本发色团的结构不变，具有传统荧光增白剂的光学性能；发色团与高分子链间的共价键使其光化学稳定性大大增强，增白性能和荧光量子产率显著提高。

In present study, we discussed the effects of cell microfilament on the activity of collagen type I alpha 1 chain gene (COL1A1) promoter under microgravity simulated by clinostat and/or cytochalasin B as microfilament depolymerizer in the established EGFP-ROS cell line using the method of fluorescence semi-quantitative analysis and the fluorescent stain of microfilament.

但微重力对相关基因调控的影响知之甚少，本研究以构建的基因工程细胞株为对象，以回转器模拟微重力效应，利用增强型绿色荧光蛋白（enhanced green fluorescence protein，EGFP）荧光半定量和细胞微丝荧光染色分析技术，探讨回转模拟微重力条件下，细胞微丝系统对I型胶原α1链基因（collagen type I alpha chain 1，COL1A1）启动子活性的影响。

And rhodamine and BODIPY dyes attract more and more attention because of their good photostability, high fluorescence quantum yields and insensitivity to pH.

而罗丹明类荧光染料和BODIPY类荧光染料由于良好的光稳定性和化学稳定性，高荧光量子产率及对pH值不敏感等优点也越来越受到人们的重视。

The interaction between diammonium glycyrrhizinate and bovine serum albumin was studied in physicological solution by spectroscopic methods including fluorescence spectra and UV visible absorption spectra. Synchronous fluorescence and three-dimensional fluorescence spectra were used to investigate the conformation change of bovine serum albumin due to the addition of diammonium glycyrrhizinate.

在模拟人体生理条件下，用常规荧光光谱和紫外－可见吸收光谱研究甘草酸二铵和牛血清白蛋白的相互作用机制，并利用同步荧光光谱和三维荧光光谱研究了药物对牛血清白蛋白构象的影响。

The reaction mechanism was studied and the proposed methods were applied to the determination of SOD activity.Second, baded on sodium terephthalate trapping HO, an automated spectrofluorimetry for determining HO with flow injection analysis was developed. It was applied to the anti-oxidation determination of maize pollen polysaccharose and natural foods.

一设计合成了两种用于超氧阴离子自由基识别检测的荧光探针——香草醛缩-8-氨基喹啉和2-吡啶甲醛苯并噻唑啉，用普通荧光法和流动注射荧光法对超氧阴离子自由基进行分析测定，研究了反应机理，并将其用于超氧阴离子自由基和超氧化物歧化酶活性的测定。

Furthermore, inhibition of TG2 by rottlerin or by the siRNA led to accumulation of green fluorescent protein-LC3-II in autophagosomes in pancreatic cancer cells transfected with GFP-LC3 (GFP-ATG8) expression vector.

此外，抑制TG2的rottlerin或小分子干扰核糖核酸导致积累的绿色荧光蛋白- LC3 - II在autophagosomes在胰腺癌细胞转染绿色荧光蛋白LC3 （绿色荧光蛋白ATG8 ）表达载体。

The immunofluorescence was developed with the mouse anti-VP4 serum. The results indicated that there was green-yellow fluorescence on the cell surface of pPG-1-VP4 and pPG-1-VP4-LTB when induced. The cells uninduced did not display an immunofluorescence reaction and were dyed red by Evans blue.

通过间接免疫荧光方法鉴定，pPG-1-VP4和pPG-1-VP4-LTB的菌体表面均能看到黄绿色荧光，而未诱导的菌体则没有荧光反应，且细胞被伊文思蓝染成红色。

The apparent permeability coefficient of FITC-albumin was calculated using the equation Pa=(1/△If)〓(r/2), where,△If is the initial step increase in fluorescent intensity,〓 is the initial rate of gradual increase in intensity as solutes diffuse out of the vessel into the extravascular space, and r is the venular radius.

荧光标记白蛋白在微血管的通透系数则通过公式Pa=(1／△If〓(r／2)，其中△If代表了初始荧光标记白蛋白浓度，〓则是荧光蛋白渗出管壁的速度，而r是血管半径。

The result showed that the maximum chlorophyll index was more suitable for the estimation of chlorophylla concentration in the water body of Taihu Lake than the fluorescence line height in the inversion analysis for the estimation of chlorophylla concentration using MERIS fluorescence parameters, and the normalized fluorescence height (NLHR681/R665) had the best fit with the measured chlorophylla concentration.

结果表明：MERIS荧光参数中最大叶绿素指数较基线荧光高度更适合太湖水体叶绿素a浓度的反演；归一化荧光高度与实测叶绿素a浓度间的拟合效果最好。

The results of its intrinsic fluorescence spectroscopy and fluorescence phase diagram showed that when the guanidine hydrochloride concentration in denaturation solution was about 1.0 mol/L, there existed a partially folded intermediate of Bacillus amyloliquefaciens a-amylase during its unfolding procedure, which followed a three-state model; the result of its fluorescence probe showed that when the guanidine hydrochloride concentration in denaturation solution was about 1.0 mol/L, there existed some stable hydrophobic regions, which could interact with a hydrophobic reagent 8-anilino-1-naphthalene sulfonic acid, in the partially folded intermediate of Bacillus amyloliquefaciens a-amylase; and the results of fluorescence quenching using acrylamide and potassium iodide as quenchers showed the distribution of Trp residues in Bacillus amyloliquefaciens a-amylase in different denaturation solution, with the maximum number (8) of tryptophan residues in a partially folded intermediate Bacillus amyloliquefaciens a-amylase molecule could be quenched by potassium iodide; and the results of their protein electrophoresis and SEC showed that no aggregate or aggregate precipitation of Bacillus amyloliquefaciens a-amylase formed during the whole unfolding procedure of Bacillus amyloliquefaciens a-amylase induced by guanidine hydrochloride.

内源荧光光谱和荧光相图结果表明，当变性液中盐酸胍浓度约为1.0 mol/L时，芽孢杆菌a-淀粉酶的去折叠过程中出现一个部分折叠中间体，其去折叠过程符合&三态模型&；荧光探针结果表明，在溶液中盐酸胍浓度约为1.0 mol/L时，中间态芽孢杆菌a-淀粉酶分子中存在着能够与探针分子1-苯胺基-8-萘磺酸结合的稳定的疏水区域；荧光猝灭研究给出了不同程度变性的淀粉液化芽孢杆菌a-淀粉酶中的Trp的分布情况，结果表明中间态芽孢杆菌a-淀粉酶分子中能够被碘化钾猝灭的位于分子表面的色氨酸残基数目达到最大的8个；蛋白电泳和体积排阻色谱结果表明，在盐酸胍诱导的芽孢杆菌a-淀粉酶分子的整个去折叠过程中，不会以共价键或非共价键形式形成芽孢杆菌a-淀粉酶分子之间的集聚体或集聚体沉淀。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。