荧光的

A new fluorescence quenching method was developed based on safranine T,a cation fluorescence dye,and used to study hybridization and dissociation of PNA-DNA for the first time.

本文建立了一种以阳离子型荧光染料藏红 T作为荧光试剂的荧光猝灭新方法，ST首次被用于PNA- DNA杂交和解链的检测。

In succession, the middle layer of anther wall had the strongest Ca^2＋ fluorescence intensity, and the tapetum took second place during the meiosis of microspore mother cell.

小孢子内Ca^2＋分布不均匀，细胞质中的Ca^2＋荧光较弱，细胞壁处Ca^2＋荧光较强，二细胞花粉时期，细胞呈现较强的Ca^2＋荧光。

A highly sensitive fluorescence quenching method for the determination of iron based on the system of iron-oxfloxacin-SDS has been developed. In NaAc-HAc medium, iron reacts with oxfloxacin and SDS to form a non-fluorescent ternary complex which can quench the fluorescence intensity of oxfloxacin.

在表面活性剂十二烷基硫酸钠存在下，铁能与氧氟沙星形成络合物而猝灭氧氟沙星分子的内源性荧光，使其荧光强度显著降低，据此，提出了铁氧氟沙星SDS体系测定铁的荧光分析新方法。

In the field of visible photosensitive resin, the absorption spectrum, excited state, solvent polarity effect, fluorescence spectrum, fluorescence quenching, concentration effect, photo fading, reaction mechanism as well as photopolymerization kinetics of xanthene iodonium salts and cyanine dye-borate were discussed. It is found that under the radiation of visible light, intra-ion-pair electron transfer reactions occur in bis salt of fluorescein and unsymmetrical trimethine cyanines containing quinoline end group triphenyl-butylboron, and the fading rate is very high.

在可见光敏树脂部分，通过对呫吨染料碘鎓盐和菁染料-硼盐体系的吸收光谱性质、激发态性质、溶剂极性效应、荧光光谱、荧光猝灭、浓度效应、光褪色反应、光聚合动力学等的研究，结果发现：荧光黄双二苯基碘鎓盐体系和含喹啉环端基不对称三甲川菁染料-三苯基丁基硼盐体系在可见光作用下均发生离子对内电子转移反应，且光褪色反应速率很高。

By using fluorescence spectrophotometry method,a systematic study was conducted to determine the effect of cadmium and lead ion stress on reactive oxygen species in creeping bent Agrotis stolonifera leaf tissue.

通过2种重金属离子和抗霉素A处理匍匐翦股颖Agrotis stolonifera幼苗叶片，利用2，'7'-二氯荧光黄双乙酸酯溶液对重金属胁迫和抗霉素A处理后叶片中产生的过氧化氢(H2O2)反应形成具荧光物质DCF的特性，用荧光光度分析法分析了叶片中H2O2产生与积累规律。

Emission spectra,spatial distribution of fluorescence,relations between chromophoric dissolved organic matter fluorescence and absorption coefficient, dissolved organic carbon concentration were studied based on the measurements from December 2004 to March 2005 in Lake Taihu.

利用紫外分光光度法和荧光法测定了2004年12月到2005年3月太湖不同湖区有色可溶性有机物的吸收和荧光，分析了CDOM荧光发光光谱、空间分布及其与吸收系数、溶解性有机碳浓度之间的关系。

When pumped with 800 nm laser irradiation, 2 {4 [2 (4 dimethylamino phenyl vinyl] phenyl} ethylamino ethanol and 2 {4 [2 (4 diethylamino phenyl vinyl] phenyl} ethylamino ethanol show strong two photon induced blue fluorescence at 436 and 440 nm respectively, while DMANHAS and 4 nitro benzoic acid 2 {4 [2 (4 diethylamino phenyl vinyl] phenyl} ethylamino ethyl ester exhibit no fluorescence.

在800 nm的飞秒脉冲激光激发下，反式-4-(N-羟乙基-N'-乙基氨基)-4'-二苯乙烯和反式-4-(N-羟乙基-N'-乙基氨基)-4'-二苯乙烯均发出较强的蓝紫色上转换荧光，荧光峰分别位于436和440 nm，而DMANHAS和反式-4-[N-(乙氧基羰基-4-硝基苯基)-N'-乙基氨基]-4'-二苯乙烯没有荧光。

Objective:Through observing the positive detect rate of hemoculture, Widal reaction,lipopolysaccha-ride passive hemagglutination assay and fluorescent quantitation polymerase chain reaction in those fever patients who have highly suspected infectious with salmonella paratyphi A.We explored the clinical value of early diagnosis of fluorescent quantitation PCR regarding paratyphoid A and evaluated its specificity,sensitivity and repeatability.

目的：通过观察血培养、Widal reaction、脂多糖—被动血凝试验及荧光定量聚合酶链反应在临床高度怀疑为甲型副伤寒沙门菌感染发热患者中的检出阳性率，初步探讨荧光定量PCR早期诊断甲型副伤寒的临床价值，并评价荧光定量PCR特异性、敏感性和可重复性。

Vascular endotheliocyte fluorescence staining and leukocyte fluorescence tracing method were applied, in combination with microscopic video system and computer imaging analyzing system, to observe in dynamic and quantitatively the influences of acupuncture on pia matral encephali microvascular morphology, density and blood flowing velocity at 3 hours, 6 hours and 24 hours after cerebral occlusion of meddle cerebral artery.morphology, density and blood flowing state at various time divisions observed directly microscopically and vascular endotheliocyte morphology and the state of brain tissue which near the microvascular after infusion with velocity in infarcted area at various time divisions measured in quantity in each group.microvascular endotheliocytes were colored worse and more fluorescent exudation appeared.

采用血管内皮细胞荧光染色及白细胞荧光示踪法，结合显微录像系统和计算机图像分析系统，动态定量地观察了针刺对大脑中动脉阻塞后3，6，24h软脑膜微血管形态、密度、血流速度的影响。主要观察指标：①从显微镜下直接观察各组大鼠不同时段软脑膜微血管的形态、密度、血流状况，以及注入荧光剂后血管内皮细胞形态和周围脑组织状况。②定量测量各组大鼠不同时段梗死区微血管密度、血流速度。结果：120只大鼠均进入结果分析。

DCHF is a nonfluorescent compound that can beoxidized to 2', 7'-dichlorofluorescin, a fluorescent product, by ROS in cells,which can be monitored by fluorescent microscope or confocal laser scanningmicroscope.

DCHF进入细胞后被ROS夺取二氢后氧化成二氯荧光素，用荧光显微镜和激光共聚焦显微镜观察DCF的荧光强度，表示ROS生成量的多少。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。