荧光的

The result of framework protein and lysosome marked with fluorescence was observed in sym-acorched laser scaning microscope: red fluorescent β-tubulin and green fluorescent eathepsin in nerve cell were seen in distribution in the same sample. The distribution of both was the same, and was located in around nucleus in the early of differentiation, and in cytoplasm and apophysis in the maturity of differentiation.

共焦激光扫描显微镜镜观察荧光双标的骨架蛋白和溶酶体的染色结果：在同一标本上，不同波长的单光激发可分别见到发红色荧光的β-tubulin和发绿色荧光的组织蛋白酶D在神经元内的存在及分布情况，二者的分布区域大致相同，神经元分化初期，较集中分布在核周附近，神经元分化成熟则分布于胞质及突起内。

Sunset clouds has comfortable pragmatic simple furniture within the red floor house , living room white hangs on the wall to oil painting having personage or scenery, bedding has the bilayer milk white quilt on bedroom 180 CM * 200 CM bed , both sides readjusts oneself to a certain extent the desk lamp having two Bixa orellana pole light yellow modelled after antiques charpie lamp shade to may adjust luminosity on the head of a bed on the bedside cupboard , the bedroom *allpox gushes the coating material blue seabed pattern having fluorescence, the queen who puts out the light in the evening may issue fluorescence , fluorescence is a dolphin and a group of fingerling.

晚霞红地面的屋内有简单舒适实用的家具，客厅白色墙上挂有人物或风景的油画，卧室180CM*200CM床上铺垫有双层奶白色被子，床头两旁床头柜上放有两盏红木仿古支杆淡黄绒布灯罩可调节光度的台灯，卧室的天花喷有荧光的蓝色海底图案的涂料，晚上熄灯后可发出荧光，荧光是一个海豚和一群小鱼。

To explore the possibility of early and rapid diagnosis of mycosisby detecting the fungal autofluorescence. Methods: To culture M. furfur and M. japonica standard strains on Dixonmedium at 32℃, and to study the autofluorescence of colonies in 2nd, 4th, 6th, 8th, 10th day under Laser Scanning Confocal Microscopy (LeicaTCS-SP2 LSCM). To study the variation of autofluorescence whenMalassezia colonies were put in 3% glutaraldehyde, pH4.0 HCL, 10% KOH, fluconazol solution, 0.3% methylene blue separately. To detect theautofluorescence of C. albicans, S. schenchii, T. rubrum, T. tonsurans, A.terreus, A. fumigatus cultured on Sabouraud dextrose agar.

选用糠秕马拉色菌和日本马拉色菌2株标准菌，接种在Dixon培养基32℃培养，分别在培养的第2、4、6、8、10天挑取菌落放在TCS-SP2型激光扫描共聚焦显微镜(Leica TCS-SP2 LSCM)下观察、测定其自发荧光；分别用3％戊二醛溶液、pH4.0盐酸溶液、10％氢氧化钾溶液、1280μg／mL和64μg／mL氟康唑溶液、0.3％美蓝溶液处理后观察马拉色菌自发荧光的变化；选择白念珠菌、申克孢子丝菌、红色毛癣菌、断发毛癣菌、土曲霉、烟曲霉等用沙堡葡萄糖琼脂培养后观察其自发荧光的特点。

Sample collection: Two hundred and fifteen sera samples of normal population were collected from the peripheral blood of healthy blood donors of Shenyang, and the sera were separated by centrifugation after blood clotting; The patients sera were collected from the outpatients and inpatients of No. 1 hospital of China Medical University and the Second hospital of Jilin University. Syphilis was confirmed by clinical and serum test of TPHA and RPR; That of pemphigus vulgarism was confirmed not only by clinical, histopathological examination, but also by direct and indirect immunofluorescence test. DIF test on lesion or non-lesion skin showed immunoglobin and/or C3 deposits in the intercellular space of epidermis, and IIF test showed positive anti- intercellular antibody in patient serum using mouse's or rat's tongue or esophagus as substrate.

标本的收集：正常人的血清：采集沈阳市正常献血员外周静脉血共215份，待血凝后离心分离血清；患者的血清：来自中国医科大学一院及吉林大学二院门诊及住院部的病人，梅毒患者除了病史及临床表现外，全部经血清学TPHA及RPR确认；寻常性天疱疮患者除了临床及组织病理诊断外，还进行了直间接免疫荧光的检测，直接免诊断疫荧光：在皮损区及非皮损区的表皮细胞间显示IgG和/或C3补体沉积；间接免疫荧光用鼠或兔的舌或食管作为底物，在病人的血清中显示有肯定的细胞间抗体。

To localize the functional positions of biomolecules in ciliates cell, we constructed a macronuclear artificial chromosome containing a gene encoding red fluorescence protein based on the structural characteristics of ciliates chromosome.

为了能够有效地分析生物大分子在该细胞中的功能作用位点，本研究根据该生物染色体结构的特征，构建了含有红色荧光蛋白的大核人工染色体EoMAC_R，并与之前构建的含绿色荧光的大核染色体EoMAC_G一起，对蛋白质合成终止有关的3个重要因子核糖体大亚基蛋白L11、多肽链释放因子eRF1和eRF3在八肋游仆虫细胞中进行了荧光共定位分析。

On the Mechanism of Populating d~3∏ State of NaK by LIF Method;2. We apply the technology of LIFto diagnose hepatic fibrosis which is induced by different matter.

本文应用激光诱导荧光的方法，对不同物质诱发白鼠的肝纤维化的肝组织进行了荧光光谱分析，发现经过注射不同物质的白鼠的肝组织与正常白鼠的肝组织的荧光光谱有明显不同。

With infection progress, the high intensity of staining was observed in the perinuclear regions and membrane area at 48hr and 72hr p.i., and the staining intensity showed high co-localization with DV2 E protein.

感染后24hr荧光的强度无明显变化，随着感染时间的延长，整合素β3抗原的荧光强度逐渐增强，在感染后48hr和72hr，细胞均呈现较强的荧光。

The direct interaction of copper ion with CuZnSOD was studied by fluorescence spectroscopy, and a type of 1:1 stable complex has been generated, which was certified by the quenching experiment of intrinsic fluorescence. The process of the generated complex shown by dynamic analysis of fluorescence quenching, consisted of two steps, in which the first step was a quick association process of double molecules to generate a loose complex, and the second step was a slow process of single molecule, where the loose complex was isomerized into the compact complex.

通过荧光光谱方法研究了这种相互作用，内源荧光的猝灭实验表明Cu(上标 2+)与CuZnSOD形成1：1型稳定配合物；荧光猝灭的动力学分析表明配合物形成过程由两个独立步骤完成：第一步是双分子快速缔合过程，形成了结合疏松的配合物，第二步是单分子慢速过程，即松散的配合物&异构化&成为结合紧密的配合物。

The direct interaction of copper ion with CuZnSOD was studied by fluorescence spectroscopy, and a type of 1∶1 stable complex has been generated, which was certified by the quenching experiment of intrinsic fluorescence. The process of the generated complex shown by dynamic analysis of fluorescence quenching, consisted of two steps, in which the first step was a quick association process of double molecules to generate a loose complex, and the second step was a slow process of single molecule, where the loose complex was isomerized into the compact complex.

通过荧光光谱方法研究了这种相互作用，内源荧光的猝灭实验表明Cu2＋与CuZnSOD形成1∶1型稳定配合物；荧光猝灭的动力学分析表明配合物形成过程由两个独立步骤完成：第一步是双分子快速缔合过程，形成了结合疏松的配合物，第二步是单分子慢速过程，即松散的配合物&异构化&成为结合紧密的配合物。

The fluorescence is also a good measurement in identifying oil inclusions and an effective indicator of its maturity,except for some oil inclusions of lower homogenization temperature with white-blue fluorescence color which have been possibly caused by heating decomposition after trapping.

本文在论述有机包裹体荧光机理的基础上，依据不同盆地样品的实验观测结果，从油包裹体荧光的强度、波长、颜色以及与有机包裹体同期的盐水包裹体的均一温度等关系进行分析，证明可以运用油包裹体荧光颜色和平均均一温度等综合指示油气的成熟度

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。