荧光的

The Cardo structure could increase the solubility and thermal stability of the resultant polyimide.

通过在聚酰亚胺的主链中引入庞大侧基（9，9-二苯基芴）形成轴状聚酰亚胺，同时提高聚酰亚胺的溶解性和热稳定性；庞大的侧基又能阻止链的堆砌，避免生色团荧光链间淬灭；通过加入第二种二酐单体合成三元缩聚型聚酰亚胺，调控荧光性二酐在聚酰亚胺中的含量，降低生色团在聚酰亚胺中的浓度，来避免生色团荧光浓度淬灭，增加聚酰亚胺的固态荧光量子效率；通过使用热稳定性好的稠环生色团苝和吖啶来避免共轭类生色团（如含双键或三键的生色团）在高温下热交联及氧和水的破坏。

A549 cells are more sensitive to CDDP-induced apoptosis(P.01) and less sensitive to THP andβ-Ele-induced apoptosis than A375p(P.01, P.05 respectively). Condensation and crack of nucleus and apoptotic bodies appeared in apoptotic cells of A549 and A375p cell lines in all treated groups and necrocytosis were to be seen in some groups. Fluorescence imaging experiments demonstrated that accumulation of iASPP in cytoplasm but little distribution in nucleus in all groups. untreated groups in two cell lines presented a bright-green colour,followed by CDDP-treated groups,and THP-treated groups is the darkest one in all groups.

免疫荧光显示，未加药物处理的A549和A375p细胞胞质染色均匀呈亮绿色，核区较暗；CDDP及β-Ele处理组细胞为暗绿色，其中可见皱缩的凋亡细胞；THP处理的细胞胞质荧光弱呈灰绿色，由于THP嵌入DNA自发红色荧光与二抗的绿色荧光中和，胞核被染成桔红色，结果提示药物处理后A549和A375p细胞中iASPP的表达有不同程度的下降。

The optical property of CdS nanocrystals in ZrO 2 thin film has been studied. The film was fabricated by sol gel technique. Photoluminescence and photoluminescence excitation spectra of the samples with different annealing temperature were measured. Photoluminescence decay curve of CdS nanocrystals at different annealing temperatures was studied.

研究了用溶胶凝胶方法制备的CdS超微粒/ZrO2薄膜的发光特性，测量了不同退火温度处理的样品的荧光光谱、荧光激发光谱和荧光衰减曲线的变化，通过荧光衰减曲线研究了140 ps左右的CdS的带间跃迁发射和较慢的表面态发射。

Ecdysis in each group of shrimp is normal. Although residue rate of the fluorescent tag has dropped along with the growth of shrimp and distribution was non uniform in body of shrimp, the maintain rate of fluorescent tag was about 95%. The effect of red fluorescence tag is the best among the three kind of VIEs.

尽管荧光标记的残留率随着虾体的生长有所下降以及分布的不均匀，但荧光标记的保持率为95%。3种颜色的荧光染料中，红色荧光标记的效果最好。

Methods (1) The device for study was determined by comparing the fluorescent images obtained by laser scanning confocal microscope and CCD fluorescent microscope respectively. The mercury-arc lamp of fluorescent microscope was studied to value its possibility to be used as the irradiation light source. Various image analyzing and processing methods were compared to determine which one was the best to be applied in this study.

(1)实验系统的建立：分别利用激光扫描共聚焦显微镜和CCD荧光显微镜采集ECV 304细胞的DCF荧光图像，并比较两者的成像质量，实验可操作性等技术指标；主要测量输出功率、功率稳定性以及光斑直径这三个指标，考察荧光显微镜的高压汞灯作为本研究中照射光源的可行性；比较并选择合适的荧光图像的分析与处理方法。

A method for the determination of BSA based on the quenching of BSA fluorescence on adding PS and a method for the determination of PS based on the enhancement of PS on adding BSA were developed. The experimental data were processed by the Stem-Volmer equation and Lineweaver-Burk double reciprocal equation.

在不同的pH条件下，酚藏花红与蛋白质的反应导致了蛋白质荧光猝灭及酚藏花红的荧光增强，其荧光猝灭值与酚藏花红的浓度成正比，可用于酚藏花红的分析测定；而酚藏花红荧光增强值与蛋白质浓度成正比，又可用于蛋白质的分析测定。

Hyphae, loaded with CTC under pH 6. 8 condition and then grown in pH 8. 0 medium for a little while to destroy the apical acidification, could gave very diffuse fluorescence images of CTC membrame bound Ca〓 and vermiform fluorescence spots due to mitochondrias under pH 6. 8 no longer were clearly distinquished, which implied that besides mitochondrias, other cellular organelles with Ca〓 also were stained with CTC. Thus mitochondrias are not the only one intracellular Ca〓 storage, endoplasmic reticulums and Golgi bodies in the same zone may be also the Ca〓 storages. But the extreme apical zone under 2μm of the growing hyphal tip was still almost devoid of stain under pH 8. 0.The CTC fluorescence was concentrated in the subapic zone beyond about 2μm from the tip and then gradually became lower behind about 40μm from the tip. These results did not support the hypothesis which suggested that the cell wall vesicles in the extreme apical zone were intracellular Ca〓 storages.

将菌丝在pH6.8条件下负载CTC，再置于pH8.0培养介质中短暂生长一会儿以消散菌丝顶端的酸化区域，则CTC膜结合Ca〓呈现弥散的荧光影像，在pH 6.8培养条件下显示的蠕虫状的线粒体荧光斑点不再能够清晰辨认，说明除了线粒体之外，还有其它含Ca〓细胞器，也被CTC染色，线粒体并不是细胞内唯一Ca〓库，还可能包括内质网、高尔基体等细胞器，但菌丝最顶端2μm以前的细胞壁泡囊区域不能被染色，最大荧光强度仍位于菌丝顶端2μm以后区域，约45μm以后荧光变弱，实验结果不支持细胞壁泡囊为菌丝胞内Ca〓库的假设。

The bay area has no substituent and has electron-withdrawing groups,such as bromine atom,cyano-group and 4-formyl phenoxy group compounds have strong absorption in 525 nm,when excitated them,they have strong yellow and salmon pink luminescence bettwen 538 and 566 nm.When introduce electron-donating substituents,such as phenoxy group,morpholinyl,piperidinyl and n-butylamino group,the absorption bathochromic shift while the electron-donating ability is improved,bettwen 536 and 692 nm have strong absorption,reach to the near-infrared region. When excitated them,only the phenoxy group compound has strong salmon pink luminescence in 572 nm,the others have no fluorescence.

其中，港湾位无取代的以及含吸电基团(—Br、—CN、对甲酰基苯氧基)化合物在525 nm左右处均有很强的吸收ε＞10~4M~(-1cm~(-1)，当光激发时，港湾位无取代、溴代和氰基取代物发出538～547 nm的强烈黄色荧光，对甲酰基苯氧基取代物则发出566 nm的强烈橙红色荧光；含供电基团(苯氧基、吗啉基、哌啶基、正丁氨基)化合物随着供电子能力的增强，吸收发生红移，在536～692 nm处均有很强的吸收ε＞10~4M~(-1cm~(-1)，达到了近红外区，当光激发时，只有苯氧基取代物发出572 nm的强烈橙红色荧光，而含氮供电基取代物均发生了荧光淬灭。

We studied the fluorescent capability of Binolate lanthanide complexes. Thefluorescent analysis shows that both ligand and complexes exhibit luminescence. Insolid state and solution, they have the different luminescence mechanism, intensity andcolor. So we can synthesize the complexes of dissimilar fluorescence performance bychanging the state and solvent polarity. According to the literatures, the complexes playan important role in luminescence material and probe with the development of theinformation technique.

对合成的八个邻萘酚稀土配合物进行发光性能研究，通过荧光光谱分析显示，配体和配合物都能够发出荧光，并且在固体状态和溶液中的发光机制、光强度及发光颜色都不同，所以通过改变邻萘酚稀土配合物的状态以及所选溶剂极性的不同，可以得到荧光性能不同的邻萘酚稀土配合物；根据查阅的文献可知，这类配合物在发光方面的应用很少，所以在当前信息显示技术高速发展的今天，邻萘酚类发光稀土配合物在发光器件、荧光探针等材料的研究领域当中不仅具有理论意义还有实际的应用价值。

It was found that the fluorescence spectra of the products of MMPB with GSH and with Cys both redshifted from λ〓/λ〓=299/355nm to λ〓/λ〓=305/425nm with the different rate. Under the following conditions: pH=7. 0 〓 buffer, T=35℃ and t=25min, the maximal fluorescence wavelengths were at λ〓/λ〓=299/355nm for MMPB-GSH and λ〓/λ〓=305/425nm for MMPB-Cys, respectively.

在pH=7.0的〓缓冲溶液中和35℃下，MMPB与GSH或Cys反应25min后，生成的MMPB-GSH的荧光峰为λ〓／λ〓=299／355nm，且此波长下的最大荧光强度可以稳定3h；而同样条件下，MMPB-Cys的荧光峰为λ〓／λ〓=305／425nm，且荧光较弱。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。