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Fluorescent decay temperature measurement principle was used to measure the temperature,for improving the performance of this kind of method as it has not yet reach the theoretical predict,an optimization method of enhancing the performance was brought forward,which including:(1) The trend of non-exponential variation of the actual fluorescent decay curve is the main factor leading to the result that the temperature measurement performance is not as good as describe by theoretical predict.

这一发现可用来开发高性能的荧光余辉测温装置;(2)提出截断归一化方法,通过图解和计算,得到荧光余辉非指数倾向程度的量化指标,用来优选荧光材料;(3)提出优化荧光余辉测温装置的设计概念,即物理现实、数学模型及数据处理方法要尽可能匹配,才能有效的提高装置测温性能;(4)仿真与实验验证了上述理论与方法的有效性。

FRAP is a technique widely usedin cell biology to observe the dynamics of biological systems, including the diffusionof membrane components.

FRAP是在细胞生物学中被广泛用于测定生物体系动态性包括生物膜组分动态扩散的一种技术,其原理是利用高强度激光将细胞内一小部分区域的荧光分子漂白,然后通过记录该区域随后的荧光恢复,检测周围未漂白区域内的荧光分子与漂白区域荧光分子的交互扩散。

First, we designed to determine the optimum ultrasound-setting, such as power level and ultrasound frequency, to achieve the optimum number of ruptures in the spinotrapezius muscles by intravital microscopy.

我们设计了应用活体荧光显微镜,测量不同条件超声介导微泡破坏作用后鼠脊斜方肌血管破裂口的数量和荧光脂质体微泡的弥散面积,确定最佳超声发射参数;然后,在慢性鼠脊斜方肌模型上,测量荧光标记蛋白的荧光强度和血管密度,对比两种血管内VEGF基因载体系统经超声介导微泡破坏作用后定向转基因表达的效果。

The results shows that quantitive variety of microorganism corresponding with forest age is not remarkable, but very remarkable with soil depth; the variety of bacterism quantity corresponding with soil depth abides by Quadratics Ratio model, the quantitive variety of actinomyces and fungi corresponding with soil depth fit to index function.

癌细胞与正常细胞的荧光光谱、荧光寿命特性及荧光峰值强度随时间变化曲线相差十分明显,反映了癌细胞与正常细胞对血卟啉亲和特性有显著的差异,测量结果确认了荧光光谱技术诊断与治疗癌症的可行性,并对发展超短脉冲激光光谱技术早期诊断与治疗癌症具有重要的指导意义和临床应用价值。

The weight ratio of riboflavin and anthrone, the weight of dispersing system LDPE and the heating time is studied during making the organic luminescent films. The possibility of achieving organic WLED using ax — 17 and zq — 13 is investigated. According to the theory of linear system, a new way of calculating the efficiency of fluorescence resonance energy transfer is investigated and it is proved by experiments.

在有机白光LED方面,本文考察了有机荧光物质核黄素和蒽酮之间质量比、分散系聚乙烯质量和成膜时间对有机发光薄膜荧光光谱的影响;探讨了使用有机荧光颜料ax—17和zq—13实现有机白光LED的可能;根据线性系统理论,提出利用供体发射谱和受体激发谱的类卷积积分估算荧光共振能量传递效率的新方法,并进行了实验验证。

After treated with the samples, the MGC-803 cells are dyed with the fluorescence dye Hoechst 33258. Observed by the fluorescence microscope, the dense dyeing granulometric and nubby blue fluorescence can be seen in the nucleus of MGC-803 treated with AK-9 and AK-11, the nucleus of some cells smash to small fragments.

采用荧光染料Hoechst 33258对经初筛活性样品处理的MGC-803细胞染色,荧光显微镜观察,发现经AK-9及AK-11发酵液处理的细胞,其细胞核内可见浓染致密的颗粒或块状荧光,细胞核发生固缩、碎裂,而对照细胞的细胞核呈均匀弥散荧光,表明这2株菌株可能产生具有诱导细胞凋亡的活性物质。

Further study of the interaction between NodD and naringenin by FRET technology is underway.

通过对naringenin分子上六个不同位点,不同性质,长度连接链以及母体和荧光团之间两种连接方式的11个荧光标记物的合成以及生物活性评价,我们找到了一个荧光标记物,它保留了野生型naringenin约20%的活性,并将其用于根瘤菌固氮过程的研究,发现该荧光标记物能够顺利进入根瘤菌活细胞中,为下一步通过FRET技术研究naringenin和NodD蛋白之间的相互作用打下了基础。

Fluorescence of NC16 and NC10NC at high pressure The monomer fluorescene intensity of the naphthalene chromophore in NC16and NC10NC in solid state and in LB film was decreased and red-shifted with theincrease in pressure, and a structureless excimer band at 415 nm was observed.

四。高压条件下NC16和NC10NC的荧光性质高压条件下研究了NC16和NC10NC在固体和在LB膜中的荧光性质,发现随压力增加,样品的单体荧光强度逐渐下降、红移;并在415 nm处出现萘的激基缔合物荧光。

Methods pna conjugated fish (fluorescence in situ hybridization)-fitc was performed on paraffin sections from melanoma,melanoma metastases and common naevi, respectively. telomere mean maximum diameter, area and number per nuclear area were calculated by image analysis on fluorescence images elaborated through confocal laser scanning microscope and fluorescence microscope.

应用肽核苷酸探针标记荧光原位杂交—荧光素异硫氰酸法检测黑色素瘤、转移黑色素瘤及黑色素痣石蜡切片中的端粒,以荧光显微镜、激光共聚焦显微镜生成图像,并详细记录细胞核平均面积、端粒数目、端粒面积。

Fluorescence resonance energy transfer is an analytical method by transfering energy between fluorescent substances.

荧光共振能量转移是一种通过荧光物质间发生荧光能量转移进行分析的实验方法。

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