荧光的

Additionally,this study developed three species-spcific real-time fluorescence quantitative PCR-based assays for rapidly, specificity, sensitivity,detecting Brucella abortus, Brucella melitensis, Brucella suis.

在建立布鲁氏菌属荧光定量PCR检测方法的基础上，根据布鲁氏菌基因组多拷贝插入元件IS711及其下游多态位点，设计了检测牛、羊、猪种布鲁氏菌的引物和牛、羊、猪种特异性荧光探针，优化了牛、羊、猪种布鲁氏菌荧光定量PCR反应体系和条件，建立了牛、羊、猪种布鲁氏菌荧光定量PCR检测方法，组装了牛、羊、猪种布鲁氏菌检测试剂盒，该方法能特异检测上述3个种的布鲁氏菌。

At 0-60 ℃, the maximum fluorescence intensity of GCS-EPF decreased on heating and the relationship between fluorescence intensity and the change of temperature was linear; At pH=0-13.5, its fluorescence intensity increased with basification and completely disappeared at very acidic pH.

结果表明，修饰后水溶性壳聚糖的水溶液、固体粉末和薄膜在520 nm附近具有较强的荧光发射，其荧光强度不仅在0-60 ℃时对度有较快敏感响应，同时在pH=0-13.5时对pH也有较快敏感响应，具有双重敏感响应，因此可将其作为温度荧光探针和pH荧光探针的高分子材料。

The fluorescence spectra, fluorescence intensities and lifetimes were investigated as a function of erbium ion concentration.

测试了掺铒碲酸盐玻璃的荧光特性，研究了4 I 13/2能级上Er3+离子的荧光谱、荧光强度和荧光寿命。

The decay process of fluorescence intensity was detected and the curve was fitted by an exponential function.

在荧光光谱峰值波长处分别监测其荧光强度随时间的衰变过程，将获得的荧光衰减动力学曲线采用指数拟合并进行解卷积处理获得不同荧光光子的寿命值。

A method for detecting the presence of a target nucleic acid sequence in a sample, said method comprising subjecting said sample to an amplification reaction using a set of nucleotides, at least one of which is fluorescently labelled, contacting amplification product with a probe under conditions in which the probe will hybridise to said target sequence, said probe comprising a reactive molecule which is able to absorb fluorescence from or donate fluorescent energy to said fluorescent labelled nucleotide and monitoring fluorescence of said sample.

一种用于检测在某样品中是否存在目标核酸序列的方法，该方法包括：使用一套核苷酸对所说的样品进行扩增反应，其中至少一个核苷酸是被荧光标记的，在探针与目标序列杂交的条件下使扩增产物与探针混合，所说的探针包括一种活性分子，该活性分子能够从荧光标记核苷酸吸收荧光或向其贡献荧光能量，及检测样品的荧光。

Expression product had a 70kDa band and emitted yellow-green luminescence in the presence of luciferin.

云南窗萤荧光素酶在大肠杆菌中表达的条带大约70kDa，并且在有荧光素存在时发出黄绿色荧光。

At different temperatures,the binding of naphthol green B to bovine serum albumin was studied by the fluorescence spectroscopy,three-dimensional fluorescence spectrum,synchronous fluorescence spectrum and ultra-violet spectrum.

在不同温度下，研究了萘酚绿B作用于牛血清白蛋白的荧光猝灭光谱、同步荧光光谱、三维荧光光谱和紫外-可见吸收光谱特征。

In this thesis, we used this principle coupled with flow injection solid phase reactor on-line oxidation, solid phase extract and dialysis separation technology to establish the on-line oxidizing fluorometric method for determination thioridazine hydrochloride and methotrexate in table and plasma samples, respectively.

第二章：研究报告一、流动注射在线氧化荧光法盐酸硫利达嗪分析体系研究流动注射在线氧化荧光法测定药物中盐酸硫利达嗪含量本方法根据盐酸硫利达嗪的氧化产物具有强荧光这一性质，在磷酸介质中利用二氧化铅固相反应器在线氧化盐酸硫利达嗪并通过乙醇对其的增敏作用，建立了流动注射在线氧化荧光法测定盐酸硫利达嗪含量的新方法。

In this thesis, fluorescent compositions of three medical materials, Bletilla striata, Star Anise Root and Lightyellow Sophora Root were studied by routine fluorescence, synchronous fluorescence and three dimension fluorescence without separation. This provides credible reference for father study of distinguishing and medical mechanism.

本文利用常规荧光，同步荧光，三维荧光，紫外光谱等多种手段对白及、八角茴香、苦参三种药材在不经分离的情况下，对其中的荧光成分进行了研究，为进一步的鉴别和药理作用研究提供了参考。

Meanwhile,the peaks of excitation spectra and fluorescence emission spectra are decided by examining the three-dimensional and two-dimensional fluorescence spectroscopy using ventricles and atriums with dye.

通过对含染料左心室组织的三维荧光光谱、荧光激发光谱和荧光发射光谱的测量，发现染料有两个荧光激发峰，分别是320nm和480 nm。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。