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A new method to measure fluorescence lifetime, which compose Lssajous figure to a straight line with fluorescence decay curve and RC curve line is described in this paper.

本文介绍了一种测量荧光寿命的新方法:利用荧光衰减曲线和RC电路衰减曲线合成李萨如图形来测量固体荧光材料的荧光寿命。

Subsequently, the tapetum cell began to disorganize, and it kept the strongest Ca^2+ fluorescence intensity from microspore to pollen maturity until the tapetum was disappeared completely.

在花药壁组织内Ca^2+分布也呈现规律性的变化:造孢组织时期,花药壁组织Ca^2+荧光强度在不同壁层组织中分布均匀;小孢子母细胞时期,药壁中层细胞Ca^2+荧光最强,绒毡层细胞次之;单核小孢子时期,绒毡层细胞呈解体状态,Ca^2+荧光最强,并保持到二核花粉时期直至绒毡层完全消失,但此时花药纤维层发育形成,表现出较强的Ca^2+荧光。

The result shows that ponceau 4R has four obvious fluorescence spectral peaks respectively located at 420,530,635 and 687 nm,each of these fluorescence spectral peaks has different best induced light,and the corresponding fluorescence spectra were listed.

文章分别从理论与实验上对220-400 nm不同激发波长下胭脂红标准溶液的荧光光谱进行了分析,结果表明,胭脂红可以产生较强的荧光,分别在420,530,635,687 nm波长处产生了4个荧光峰,各荧光峰的最佳激发波长也不尽相同,文章给出了相应的荧光光谱图。

The synthesized monomer and fluorescent polymers were characterized by the methods of nuclear magnetic resonance (~1H NMR), mass spectrography, fourier transform infrared spectroscopy, ultra-violet-visible spectra and differential scanning calorimetry, respectively.

采用核磁(~1H NMR),质谱,红外光谱,差示扫描量热法,可见紫外分光光度仪和荧光光谱等方法对合成的荧光分子以及荧光聚合物的结构和性能做了表征,系统地研究了所合成的荧光聚合物的荧光性能。

Thin leaf section were prepared from fresh tissues of YS-lnfected and healthy citrus plants, when observed under a fluorescence microscope (Type, Olympus AH-FL-2), a yellow fluorescence was distinctly observed in the YS-infected phloem tissues and not in the phloem tissuses of healthy plants and of those infected with tristeza, exocortis, canker, scab, greasy...

将健叶和黄龙病叶叶柄用保险刀片徒手切片,在透射式荧光显微镜下检查,可看到健叶叶柄的木质部导管细胞壁发黄色荧光,韧皮纤维细胞发绿色荧光,而病叶叶柄韧皮部中有1~多个鲜明的黄色或黄绿色荧光团块。这种特异性的荧光,在健叶柄韧皮部中是没有的,其它由病毒、类病毒、细菌、真菌等病原引起的病叶柄韧皮部中亦没有此种特异性荧光。

To establish new methods for highly sensitive time-resolved fluorescence bioassay, several new lanthanide fluorescence complexes were synthesized and used for the preparations of nanometer-sized lanthanide fluorescence biolabels. Novel lanthanide fluorescence nano-biolabels are uniform (25-55 nm in diameters),strongly fluorescent with long fluorescence lifetime, highly photostable and biocompatible.

本项目以时间分辨荧光生物分析新方法的建立与应用为研究目的,在合成出几种稀土荧光配合物的基础上,建立了几种新型强荧光性纳米稀土荧光生物标记材料的可控制备方法,生物标记方法及其在时间分辨荧光免疫分析中的应用方法。

To establish new methods for highly sensitive time-resolved fluorescence bioassay, several new lanthanide fluorescence complexes were synthesized and used for the preparations of nanometer-sized lanthanide fluorescence biolabels. Novel lanthanide fluorescence nano-biolabels are uniform (25-55 nm in diameters),strongly fluorescent with long fluorescence lifetime, highly photostable and biocompatible.

中文摘要:本项目以时间分辨荧光生物分析新方法的建立与应用为研究目的,在合成出几种稀土荧光配合物的基础上,建立了几种新型强荧光性纳米稀土荧光生物标记材料的可控制备方法,生物标记方法及其在时间分辨荧光免疫分析中的应用方法。

Moreover, their optimum excitation wavelength and emission wavelength are studied and chosen. The fluorescent property of carbaryl and metolcarb in different pH was studied, and the working curve was established according to the optimized experiment condition.

研究了农药的分子结构与其自然荧光特性关系,实验研究了这几种农药的激发光谱、荧光光谱特性以及本底溶剂的荧光光谱特性,研究并选择了它们的最佳激发和荧光发射参数,实验研究了不同pH值下西维因、速灭威等农药的荧光特性,测定了农药工作曲线。

Flow injection fluorometry for the determination of carbamazepine in pharmaceuticals and plasmaA simple, sensitive and specific spectrofluorimetric method has been developed for the determination of CBZ. The method was based on lead dioxide solid-phase reactor flow injection on-line oxidizing CBZ into fluorescent product in medium of phosphoric acid. The product has green-yellow fluorescence at excitation wavelength of 355 nm and emission wavelength of 478 nm, and the fluorescence intensity could be enhanced in the presence of acetone. The reaction conditions and experimental conditions were studied and optimized.

二、流动注射在线氧化荧光法测定卡马西平的研究利用卡马西平自身无荧光,经过氧化后可转变为在最大激发波长为355nm,最大发射波长为478nm的强荧光物质,而且丙酮对该荧光体系有增敏作用的现象,应用流动注射体系建立了以醋酸纤维素固定化的二氧化铅作为固相反应器、在磷酸介质中在线氧化荧光法测定卡马西平的新方法。

The results show that the as-synthesized chalcone-containing copolymers are a kind of thermosensitive polymer with a lower critic solution temperature and the LCST of the copolymer decreases with the increase of the chalcone content in the copolymer. With the increase of the solvent polarity, both the maximum absorption wavelength and the fluorescence emitting wavelength of the chalcone-containing copolymers redshifts, and the intensity of fluorescence first increases, then decreases, which exhibits solvent polarity sensitive fluorescence properties.

结果表明,侧链查尔酮共聚物是一类具有低临界溶解温度的温敏性聚合物,其LCST温度随着共聚物中查尔酮含量的增加而降低;随着溶剂极性的增加,侧链查尔酮共聚物的紫外-可见最大吸收波长红移,其荧光发光波长红移并且发光强度先增强后降低,具有溶剂极性敏感的荧光特性;同时对比侧链查尔酮共聚物水溶液低温和高温下的荧光,发现低温下几乎无荧光,高温下其荧光得到明显增强,其荧光具有可逆的温度&开/关&特性。

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The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应,而且减少跟风的可能性。

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