荧光的

The interactions of the model compound (FL4) or diad composed of fluorescein and 2-methyl-anthraquinone and TiO_2 colloid have been examined by absorption, fluorescence spectroscopy and fluorescence lifetime.

测定了以荧光素为光敏剂的电子给体-受体二元化合物荧光素蒽醌甲酯及模型化合物荧光素丁酯（FL4）在溶液中和在二氧化钛（TiO_2）胶体中的吸收光谱、荧光光谱、荧光寿命等。

It is found that the fluorescence intensity is increased with the elevation of temperature when the quencher diphenylamine is added to PU-DFY, which is an inverse phenomenon against the general fluorescent materials.

研究还发现，加入二苯胺猝灭剂的PU-DFY，荧光强度随温度的升高而增强，与一般荧光物质荧光强度的温度特性相反。关键词：聚氨酯-分散荧光黄；高分子荧光染料；水分散体；荧光

Based on the previous literatures, the following major innovation works were carried out in this dissertation:(1) The derivatization reaction conditions of ephedrine and pseudoephedrine with sensitive derivation reagent in aqueous system were studied and two new methods were developed for the assay of them by microemulsion electrokinetic chromatography and micellar electrokinetic chromatography with LIF detection. The sensitivity and analysis times were greatly improved compared with the previous reports;(2) A new method of CZE with indirect LIF detection was developed for the simultaneous determination of six coumarin compounds (esculin, esculetin, isofraxidin, genistein, naringin and sophoricoside) with fluorescein as the probe. The proposed method enlarges the application range of LIF detector, and provides new approach for the analysis of certain compounds difficult to derivatize;(3) Based on the above research, an MEKC with indirect LIF detection method for the simultaneous determination of adenine and guanine in DNA extracts from fungus, maize and soybean was established;(4) A new method for the investigation of the complexes formed between human serum albumin and ampicillin sodium under the simulated physiology conditions using laser light scattering technique was developed.

该论文在综述前人工作的基础上，开展了如下未见文献报道的创新性的研究工作：(1)使用灵敏的衍生试剂对麻黄碱和伪麻黄碱在水体系中的衍生反应条件进行了系统研究，建立了微乳电动色谱-激光诱导荧光检测法和胶束电动色谱—激光诱导荧光检测法测定麻黄碱和伪麻黄碱的灵敏分析新方法，与以前的报道相比，灵敏度和分析时间均有很大改善；(2)使用荧光素钠作为背景荧光试剂，建立了同时分析测定六种黄酮类化合物(秦皮甲素、秦皮乙素、异秦皮定、染料木素、柚皮苷和槐角苷)的毛细管区带电泳—间接激光诱导荧光检测新方法，扩大了激光诱导荧光检测的应用范围，对难衍生化合物的分离分析提供了一种新思路和新途径；(3)以荧光素钠作为背景荧光试剂，建立了一种用于同时测定食品提取DNA中的腺嘌呤和鸟嘌呤含量的胶束电动色谱—间接激光诱导荧光检测新方法；(4)用动态和静态激光散射法研究了模拟生理条件下人血清白蛋白与氨比西林钠盐相互作用所形成的复合物，为药物分子与蛋白质的相互作用、药代动力学等的研究提供了一种新思路。

In the field condition, PAM-2100 chlorophyll fluorometer was used to observe chlorophyll fluorescence kinetics parameters of 18 winter wheat varieties in different growth stages, the correlations of 14 fluorescence parameters such as Fo were analyzed. Fluorescence characteristics of winter wheat varieties were comprehensive evaluated by the methods of factor analysis and cluster analysis.

在大田条件下应用PAM-2100型叶绿素荧光仪对18个冬小麦品种叶绿素荧光动力学参数进行了不同生育期的观测，分析冬小麦叶绿素荧光参数Fo等14个荧光参数间的相关关系，利用因子分析和聚类分析对其冬小麦品种荧光特性进行综合评价。

Firefly can give rise to luminescence during the night, the basic reaction involved the firefly luciferase and luciferin and ATP in the body of firefly.

萤火虫在夜晚可以发出荧光，其基本的生物化学反应是虫荧光素酶可以催化荧光素生成氧化荧光素并且放出荧光，可以用以下这个反应方程表示：荧光素+ATP+O_2？

In this paper, we studied the relationshipbetween the concentration, acidity, temperature, heavy metalic ions and the fluorescencequenching of fluorescein. The result showed that when the concentration was over 1. 55 ×10-5 mol/L, the fluorescence quenching strengthened with the increase of f...

结果表明：荧光素浓度大于1.55×10－5mol·L－1时，随着浓度增大，荧光猝灭作用增强；酸度对荧光猝灭作用有明显作用，PH值小于8.5时，随着PH值升高而减弱，PH值大于8.5时，随着PH值升高而增强；温度对荧光猝灭作用呈线性关系，温度升高，荧光猝灭作用减弱；重金属离子对荧光猝灭有显著的作用，是影响眼底血管造影质量的重要因素。

The results show that ponceau 4R excited by light at the wavelength of 330-430 nm can generate a strong fluorescence at the 621 nm peak wavelength with its best excitation wavelength being 376 nm, amaranth excited by light at the wavelength of 300-440 nm can generate a strong fluorescence at the 643 nm peak wavelength with its best excitation wavelength being 370 nm, tartrazine excited by light at the wavelength of 280-380 nm can generate a strong fluorescence at the 565 nm peak wavelength with its best excitation wavelength being 315 nm, sunset yellow excited by light with wavelength of 310-410 nm can generate a strong fluorescence at the 592 nm peak wavelength with its best excitation wavelength being 348 nm, and brilliant blue excited by light at the wavelength of 320-390 nm can generate a strong fluorescence at the 456 nm peak wavelength with its best excitation wavelength being 350 nm.

结果表明，胭脂红在波长330～430 nm的光激发下，产生较强荧光，荧光峰值波长为621 nm，最佳激发波长为376 nm；苋菜红在波长300～440 nm的光激发下，产生较强荧光，荧光峰值波长为643 nm，最佳激发波长为370 nm；柠檬黄在波长280～380 nm的光激发下，产生很强荧光，荧光峰值波长为565 nm，最佳激发波长为315 nm；日落黄在波长310～410 nm的光激发下，产生较强荧光，荧光峰值波长为592 nm，最佳激发波长为348 nm；亮蓝在波长320～390 nm的光激发下，产生较强荧光，荧光峰值波长为456 nm，最佳激发波长为350 nm。

These observations suggest that PVA-MEC is an excellent fluorescent macromolecular material with a convenient method of preparation and had a good water-soluble ability.(2) An acrylic monomer bearing xanthene group, acryloyl-fluorescein was synthesized from fluorescein and acryloyl chloride. The photophysical behaviors of Ac-Flu and its copolymer were explored by recording the fluorescence spectra in solution, solid state and film in detail.

2我们采用基于荧光功能单体制备荧光聚合物的方法，选择荧光素为发色团，使它与丙烯酰氯反应，合成出含有双键的荧光素单体Ac-Flu，然后令其与丙烯酰胺共聚，制备得到含有荧光素生色团的水溶性荧光聚合物poly(Ac-Flu-co-AM)。

The results showed that: three major peaks,humuslike fluorescence peak A,proteinlike fluorescence peaks B and C appeared on the threedimensional fluorescence spectroscopy;the DOM fluorescence intensity was well related with DOC and CODMn,indicating that fluorescence dissolved organic matter content can reflect both the organic carbon content and the organic matter content by oxidation in the water in August,during the nonalgae bloom outbreak period,fluorescence intensity had a poor correlation with chlorophyll a,indicating that phytoplankton was not the main source of the fluorescent substances.

结果显示：小江回水区DOM的三维荧光光谱主要表现出3个峰，类腐殖质荧光峰A、类蛋白荧光峰B和C；DOM荧光强度与DOC、CODMn相关性较好，表明荧光溶解有机物质的含量可以较好反映水体中有机碳和可被氧化有机物的含量；在8月份非水华暴发时期荧光强度和叶绿素a相关性较差，表明DOM荧光物质主要不是由浮游植物产生。

All metaphases showed three distinct green signals, which have the same size and intensity, corresponding to a normal chromosome 8 and two isochromosomes 8q, and five red signals, of which one was located on a normal chromosome 8 and the another two quite symmetrically on the both arms of each isochromosome 8q, respectively.

结果发现：所有中期分裂相均显示3个绿色荧光杂交信号和5个红色荧光杂交信号；2个绿色荧光杂交信号分别位于2条等臂8q染色体着丝粒处，信号大小和强度均与位于正常8号染色体着丝粒处的1个绿色荧光杂交信号相当；1个红色荧光杂交信号位于正常8号染色体长臂上，另外2个红色荧光杂交信号非常对称地位于每一条等臂8q染色体的两条长臂上。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。