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To establish a real-time PCR method for the rapid detection of Listeria monocytogenes in simulated clinical specimens.

本研究利用荧光定量PCR(real-time PCR)技术,建立针对临床标本中单增李斯特菌的快速检测方法。

Fluorospectorphotometer was employed to investigate the intracellular concentration of rhodamine123 to reflect the effect of lobeline on the activity of MDR-related protein P-glycoprotein.

多功能酶标仪测定洛贝林干预对细胞内罗丹明123荧光强度以反映其对细胞多药耐药蛋白P-gp活性的影响。

The fluorescence data were treated by using the double logarithmic equation, and the binding constants K of the interaction of CPFX with trypsin and the number of binding sites n were obtained.

根据双对数方程处理荧光猝灭数据得到了CPFX与Trypsin在不同温度下的结合常数K和结合位点数n。

We addressed this question with the method of luciferase reporter gene.

我们用荧光素酶报告基因的方法,探讨了这个问题。

Luciferase assay was used to study the impact of CMTM1-v17 on the transactivation of AR and its mechanism.

用荧光素酶报告系统研究CMTM1-v17对AR转录活性的影响及作用机制。

These expression vectors were transfected into cells and the relative luciferase activity was measured 48h later.

这些表达载体转染细胞的相对荧光素酶活性测定48小时后。

Results: Expression of ERα induced the luciferase activity in a dose-dependent manner.

雌激素处理后,荧光素酶的活性无显著变化。

The details of cloning, expression and function of biotinylated luciferase will be discussed in this paper.

本文将就生物素化荧光素酶的克隆、表达以及功能检测进行具体讨论。

Experimentally, with luciferase assays, we demonstrate average repressions of 30% or more for 168 of 226 tested targets.

利用荧光素酶分析方法,他们确定出这种方法对226个被检测靶标中的168个靶标的平均抑制率达30%或更高。

Result The fold activation value of luciferase of rifampin group was statistically different from control group and DMSO group.

结果经利福平处理的细胞裂解物荧光素酶比活性值与阴性对照组和溶剂组差异显著。

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The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应,而且减少跟风的可能性。

The new PS20 solar power tower collected sunlight through mirrors known as "heliostats" to produce steam that is converted into electricity by a turbine in Sanlucar la Mayor, Spain, Wednesday.

聚光:照片上是建在西班牙桑路卡拉马尤城的一座新型PS20塔式太阳能电站。被称为&日光反射装置&的镜子将太阳光反射到主塔,然后用聚集的热量产生蒸汽进而通过涡轮机转化为电力